Supplementary information files for 'Sheath-flow probe electrospray ionization (sfPESI) mass spectrometry for the rapid forensic analysis of human body fluids'

Supplementary information files for 'Sheath-flow probe electrospray ionization (sfPESI) mass spectrometry for the rapid forensic analysis of human body fluids'Abstract:Sheath-flow probe electrospray ionisation (sfPESI) has for the first time been applied to the analysis of both fresh and dried human blood, saliva and urine. sfPESI enables the in situ sampling of biological materials with no sample preparation, demonstrating a promising technique for the rapid analysis and identification of body fluids of forensic interest.</div

Baseline demographic, lifestyle and clinical characteristics of study participants included at various study follow-up phases.^*

<p>Abbreviations: BMI, body mass index (calculated as weight in kilograms divided by height in meters squared); MDD, major depressive disorder; OCP, oral contraceptive pill.</p>*<p>All values expressed as mean (SD), unless otherwise specified.</p>‡<p>Calcium intake calculated from dietary and supplement sources.</p>§<p>Index of physical fitness, meters covered in 12-minut</p

Study flow diagram.

<p>Note: The number of exclusions does not match the number of people as some participants were found to have more than one exclusionary criterion.</p

BMD values adjusted by BMI in women with depression versus control women at each main skeletal site.^*

<p>BMD values adjusted by BMI in women with depression versus control women at each main skeletal site.^*</p

Bone mineral density measurements in women with MDD and moderate osteopenia or osteoporosis randomized to alendronate <i>vs.</i> placebo.

<p>Over 12 months, the Alendronate group showed a significant increase in BMD at the lumbar spine (P = 0.003), and there was a trend for increased BMD at the femoral neck (P = 0.06). No changes over time were observed in the Placebo group.</p

Calcium metabolism, bone turnover markers, and hormonal measurements in women with depression compared to control women.^*

*<p>All values expressed as mean (SD), unless otherwise specified.</p>‡<p>25-Hydroxyvitamin D, ng/mL levels were only obtained at baseline.</p><p>Sample size indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040894#pone-0040894-t001" target="_blank">Table 1</a>.</p

Circadian profiles of plasma ACTH, cortisol, and leptin.

<p>Circadian profiles of plasma ACTH, cortisol, and leptin.</p

Clinical characteristics of depressive subtypes of women with unipolar MDD.

<p>Values are reported as mean ± SD or percent.</p><p>Sample size in parenthesis, unless otherwise indicated.</p><p>Significant comparisons, assessed as p≤.05 nominal value.</p>b<p>Atypical differed from undifferentiated.</p

Synopsis of significant findings in the three subtypes of depression vs. healthy controls.

<p>Synopsis of significant findings in the three subtypes of depression vs. healthy controls.</p

Demographic characteristics of depressive subtypes of women with unipolar MDD.

<p>Values are reported as mean ± SD or percent.</p><p>Sample size in parenthesis, unless otherwise indicated.</p><p>Significant comparisons, assessed as p≤.05 nominal value.</p><p><b>Overall</b>: overall test.</p>a<p>Atypical differs from control.</p>b<p>Undifferentiated differs from control.</p