1 research outputs found
Quantitative Analysis and Discovery of Lysine and Arginine Modifications
Post-translational
modifications (PTMs) affect protein function,
localization, and stability, yet very little is known about the ratios
of these modifications. Here, we describe a novel method to quantitate
and assess the relative stoichiometry of Lys and Arg modifications
(QuARKMod) in complex biological settings. We demonstrate the versatility
of this platform in monitoring recombinant protein modification of
peptide substrates, PTMs of individual histones, and the relative
abundance of these PTMs as a function of subcellular location. Lastly,
we describe a product ion scanning technique that offers the potential
to discover unexpected and possibly novel Lys and Arg modifications.
In summary, this approach yields accurate quantitation and discovery
of protein PTMs in complex biological systems without the requirement
of high mass accuracy instrumentation