A High-Density Linkage Map of the Ancestral Diploid Strawberry, Fragaria iinumae, Constructed with Single Nucleotide Polymorphism Markers from the IStraw90 Array and Genotyping by Sequencing

Fragaria iinumae Makino is recognized as an ancestor of the octoploid strawberry species, which includes the cultivated strawberry, Fragaria ×ananassa Duchesne ex Rozier. Here we report the construction of the first high-density linkage map for F. iinumae. The F. iinumae linkage map (Fii map) is based on two high-throughput techniques of single nucleotide polymorphism (SNP) genotyping: the IStraw90 Array (hereafter “Array”), and genotyping by sequencing (GBS). The F2 generation mapping population was derived by selfing F. iinumae hybrid F1D, the product of a cross between two divergent F. iinumae accessions collected from Hokkaido, Japan. The Fii map consists of seven linkage groups (LGs) and has an overall length of 451.7 cM as defined by 496 loci populated by 4173 markers: 3280 from the Array and 893 from GBS. Comparisons with two versions of the Fragaria vesca ssp. vesca L. ‘Hawaii 4’ pseudo-chromosome (PC) assemblies reveal substantial conservation of synteny and colinearity, yet identified differences that point to possible genomic divergences between F. iinumae and F. vesca, and/or to F. vesca genomic assembly errors. The Fii map provides a basis for anchoring a F. iinumae genome assembly as a prerequisite for constructing a second diploid reference genome for Fragaria

Matters of Ethics, Trust, and Potential Liability for Autonomous Systems

Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.The objective of this panel was to discuss issues related to the development and use of autonomous systems, with specific focus on the overriding themes of ethical considerations and potential liability for Human Factors and Ergonomics (HF/E) professionals who are involved in their development. Chris Brill provided opening remarks to frame the discussion and introduce the panelists. James Bliss discussed legal implications related to our collective penchant for developing conservative, false-alarm prone automation. Peter Hancock advocated for human-centered constraints on autonomous systems, as they may, one day, pose an existential threat to humanity. Dietrich Manzey discussed ethical considerations for autonomous systems, including how design can encourage ethical user behavior. Joachim Meyer argued that HF/E professionals have an obligation to help designers understand the ethical implications of poor design, particularly in the context of autonomous systems. Lastly, Alison Vredenburgh provided thoughts on potential liability for HF/E professionals, particularly in light of the relative newness of autonomous systems. The panel then turned to facilitated discussion with panelists and audience members. Specific themes included the boundaries of our responsibilities as HF/E professionals for ill-conceived or morally-objectionable systems, potential implications of manipulating user trust through design, cross-cultural perspectives on public acceptance and legal peril, and how concerns might differ by domain (e.g., medical vs. combat vs. manufacturing). The session concluded with panelists summarizing how ethics influence design and recommendations for how HF/E professionals can potentially protect themselves from legal liability for mishaps involving autonomous systems they helped develop

WHIRLY1 functions in the nucleus to regulate barley leaf development and associated metabolite profiles

The WHIRLY (WHY) DNA/RNA binding proteins fulfil multiple but poorly characterised functions in leaf development. Here, we show that WHY1 transcript levels were highest in the bases of 7-day old barley leaves. Immunogold labelling revealed that the WHY1 protein was more abundant in the nuclei than the proplastids of the leaf bases. To identify transcripts associated with leaf development we conducted hierarchical clustering of differentially abundant transcripts along the developmental gradient of wild-type leaves. Similarly, metabolite profiling was employed to identify metabolites exhibiting a developmental gradient. A comparative analysis of transcripts and metabolites in barley lines (W1–1 and W1–7) lacking WHY1, which show delayed greening compared with the wild type revealed that the transcript profile of leaf development was largely unchanged in W1–1 and W1–7 leaves. However, there were differences in levels of several transcripts encoding transcription factors associated with chloroplast development. These include a barley homologue of the Arabidopsis GATA transcription factor that regulates stomatal development, greening and chloroplast development, NAC1; two transcripts with similarity to Arabidopsis GLK1 and two transcripts encoding ARF transcriptions factors with functions in leaf morphogenesis and development. Chloroplast proteins were less abundant in the W1–1 and W1–7 leaves than the wild type. The levels of tricarboxylic acid cycle metabolites and GABA were significantly lower in WHY1 knockdown leaves than the wild type. This study provides evidence that WHY1 is localised in the nuclei of leaf bases, contributing the regulation of nuclear-encoded transcripts that regulate chloroplast development

The amyloid-β1-42-oligomer interacting peptide D-AIP possesses favorable biostability, pharmacokinetics, and brain region distribution.

We have previously developed a unique 8-amino acid Aβ42 oligomer-Interacting Peptide (AIP) as a novel anti-amyloid strategy for the treatment of Alzheimer's disease. Our lead candidate has successfully progressed from test tubes (i.e., in vitro characterization of protease-resistant D-AIP) to transgenic flies (i.e., in vivo rescue of human Aβ42-mediated toxicity via D-AIP-supplemented food). In the present study, we examined D-AIP in terms of its stability in multiple biological matrices (i.e., ex-vivo mouse plasma, whole blood, and liver S9 fractions) using MALDI mass spectrometry, pharmacokinetics using a rapid and sensitive LC-MS method, and blood brain barrier (BBB) penetrance in WT C57LB/6 mice. D-AIP was found to be relatively stable over 3 h at 37 °C in all matrices tested. Finally, label-free MALDI imaging showed that orally administered D-AIP can readily penetrate the intact BBB in both male and female WT mice. Based upon the favorable stability, pharmacokinetics, and BBB penetration outcomes for orally administered D-AIP in WT mice, we then examined the effect of D-AIP on amyloid "seeding" in vitro (i.e., freshly monomerized versus preaggregated Aβ42). Complementary biophysical assays (ThT, TEM, and MALDI-TOF MS) showed that D-AIP can directly interact with synthetic Aβ42 aggregates to disrupt primary and/or secondary seeding events. Taken together, the unique mechanistic and desired therapeutic potential of our lead D-AIP candidate warrants further investigation, that is, testing of D-AIP efficacy on the altered amyloid/tau pathology in transgenic mouse models of Alzheimer's disease

Jetstream: A self-provisoned, scalable science and engineering cloud environment

The paper describes the motivation behind Jetstream, its functions, hardware configuration, software environment, user interface, design, use cases, relationships with other projects such as Wrangler and iPlant, and challenges in implementation.Funded by the National Science Foundation Award #ACI - 144560

A targeted search for repeating fast radio bursts with the MWA

We present a targeted search for low-frequency (144--215\,MHz) FRB emission from five repeating FRBs using 23.3\,hr of archival data taken with the Murchison Widefield Array (MWA) Voltage Capture System (VCS) between 2014 September and 2020 May. This is the first time that the MWA VCS has been used to search for FRB signals from known repeaters, which enables much more sensitive FRB searches than previously performed with the standard MWA correlator mode. We performed a standard single pulse search with a temporal and spectral resolution of $400\,\mu$s and 10\,kHz, respectively, over a $100\,\text{pc}\,\text{cm}^{-3}$ dispersion measure (DM) range centred at the known DM of each studied repeating FRB. No FRBs exceeding a $6\sigma$ threshold were detected. The fluence upper limits in the range of 32--1175\,Jy\,ms and 36--488\,Jy\,ms derived from 10 observations of FRB 20190711A and four observations of FRB 20201124A respectively, allow us to constrain the spectral indices of their bursts to $\gtrsim-1$ if these two repeaters were active during the MWA observations. If free-free absorption is responsible for our non-detection, we can constrain the size of the absorbing medium in terms of the electron temperature $T$ to $<1.00\times(T/10^4\text{K})^{-1.35}\,\text{pc}$, $<0.92\times(T/10^4\text{K})^{-1.35}\,\text{pc}$ and $<[0.22\text{--}2.50]\times(T/10^4\text{K})^{-1.35}\,\text{pc}$ for FRB 20190117A, 20190711A, and 20201124A, respectively. However, given that the activities of these repeaters are not well characterised, our non-detections could also suggest they were inactive during the MWA observations.Comment: Accepted for publication in MNRA