4,002 research outputs found
A Coproantigen Diagnostic Test for Strongyloides Infection
Accurate diagnosis of infection with the parasite Strongyloides stercoralis is hampered by the low concentration of larvae in stool, rendering parasitological diagnosis insensitive. Even if the more sensitive agar plate culture method is used repeated stool sampling is necessary to achieve satisfactory sensitivity. In this manuscript we describe the development of a coproantigen ELISA for diagnosis of infection. Polyclonal rabbit antiserum was raised against Strongyloides ratti excretory/secretory (E/S) antigen and utilized to develop an antigen capture ELISA. The assay enabled detection of subpatent rodent S. ratti and human S. stercoralis infection. No cross-reactivity was observed with purified E/S from Schistosoma japonicum, the hookworms Ancylostoma caninum, A. ceylanicum, nor with fecal samples collected from rodents harboring Trichuris muris or S. mansoni infection. Strongyloides coproantigens that appear stable when frozen as formalin-extracted fecal supernatants stored at −20°C remained positive up to 270 days of storage, whereas supernatants stored at 4°C tested negative. These results indicate that diagnosis of human strongyloidiasis by detection of coproantigen is an approach worthy of further development
Rotations and Abundances of Blue Horizontal-Branch Stars in Globular Cluster M15
High-resolution optical spectra of eighteen blue horizontal-branch (BHB)
stars in the globular cluster M15 indicate that their stellar rotation rates
and photospheric compositions vary strongly as a function of effective
temperature. Among the cooler stars in the sample, at Teff ~ 8500 K, metal
abundances are in rough agreement with the canonical cluster metallicity, and
the v sin i rotations appear to have a bimodal distribution, with eight stars
at v sin i < 15 km/s and two stars at v sin i ~ 35 km/s. Most of the stars at
Teff > 10000 K, however, are slowly rotating, v sin i < 7 km/s, and their iron
and titanium are enhanced by a factor of 300 to solar abundance levels.
Magnesium maintains a nearly constant abundance over the entire range of Teff,
and helium is depleted by factors of 10 to 30 in three of the hotter stars.
Diffusion effects in the stellar atmospheres are the most likely explanation
for these large differences in composition. Our results are qualitatively very
similar to those previously reported for M13 and NGC 6752, but with even larger
enhancement amplitudes, presumably due to the increased efficiency of radiative
levitation at lower intrinsic [Fe/H]. We also see evidence for faster stellar
rotation explicitly preventing the onset of the diffusion mechanisms among a
subset of the hotter stars.Comment: 11 pages, 1 figure, 1 table, accepted to ApJ
Striking Photospheric Abundance Anomalies in Blue Horizontal-Branch Stars in Globular Cluster M13
High-resolution optical spectra of thirteen blue horizontal-branch (BHB)
stars in the globular cluster M13 show enormous deviations in element
abundances from the expected cluster metallicity. In the hotter stars (T_eff >
12000 K), helium is depleted by factors of 10 to 100 below solar, while iron is
enhanced to three times the solar abundance, two orders of magnitude above the
canonical metallicity [Fe/H] ~= -1.5 dex for this globular cluster. Nitrogen,
phosphorus, and chromium exhibit even more pronounced enhancements, and other
metals are also mildly overabundant, with the exception of magnesium, which
stays very near the expected cluster metallicity. These photospheric anomalies
are most likely due to diffusion --- gravitational settling of helium, and
radiative levitation of the other elements --- in the stable radiative
atmospheres of these hot stars. The effects of these mechanisms may have some
impact on the photometric morphology of the cluster's horizontal branch and on
estimates of its age and distance.Comment: 11 pages, 1 Postscript figure, uses aaspp4.sty, accepted for
publication in ApJ Letter
Experimental human hookworm infection: a narrative historical review
In 1896, a serendipitous laboratory accident led to the understanding that hookworms propagate infection by penetrating skin, a theory that was then confirmed with the first experimental human infection, reported in 1901. Experimental human infections undertaken in the 20th century enabled understanding of the natural history of infection and the immune response. More recently, experimental hookworm infection has been performed to investigate the immunomodulatory potential of hookworm infection and for the evaluation of hookworm vaccines and chemotherapeutic interventions. Experimental human hookworm infection has been proven to be safe, with no deaths observed in over 500 participants (although early reports predate systematic adverse event reporting) and no serious adverse events described in over 200 participants enrolled in contemporary clinical trials. While experimental human hookworm infection holds significant promise, as both a challenge model for testing anti-hookworm therapies and for treating various diseases of modernity, there are many challenges that present. These challenges include preparation and storage of larvae, which has not significantly changed since Harada and Mori first described their coproculture method in 1955. In vitro methods of hookworm larval culture, storage, and the development of meaningful potency or release assays are required. Surrogate markers of intestinal infection intensity are required because faecal egg counts or hookworm faecal DNA intensity lack the fidelity required for exploration of hookworm infection as a vaccine/drug testing platform or as a regulated therapy
Modelling the dynamics of Plasmodium falciparum histidine-rich protein 2 in human malaria to better understand malaria rapid diagnostic test performance
Background: Effective diagnosis of malaria is a major component of case management. Rapid diagnostic tests (RDTs) based on Plasmodium falciparumhistidine-rich protein 2 (PfHRP2) are popular for diagnosis of this most virulent malaria infection. However, concerns have been raised about the longevity of the PfHRP2 antigenaemia following curative treatment in endemic regions. Methods. A model of PfHRP2 production and decay was developed to mimic the kinetics of PfHRP2 antigenaemia during infections. Data from two human infection studies was used to fit the model, and to investigate PfHRP2 kinetics. Four malaria RDTs were assessed in the laboratory to determine the minimum detectable concentration of PfHRP2. Results: Fitting of the PfHRP2 dynamics model indicated that in malaria nave hosts, P. falciparum parasites of the 3D7 strain produce 1.4 × 10 g of PfHRP2 per parasite per replication cycle. The four RDTs had minimum detection thresholds between 6.9 and 27.8 ng/mL. Combining these detection thresholds with the kinetics of PfHRP2, it is predicted that as few as 8 parasites/L may be required to maintain a positive RDT in a chronic infection. Conclusions: The results of the model indicate that good quality PfHRP2-based RDTs should be able to detect parasites on the first day of symptoms, and that the persistence of the antigen will cause the tests to remain positive for at least seven days after treatment. The duration of a positive test result following curative treatment is dependent on the duration and density of parasitaemia prior to treatment and the presence and affinity of anti-PfHRP2 antibodies
Not All Worms Were Created Equal
[Extract] Whilst we support the need to report safety outcomes from experimental therapy of any kind, including the use of helminths as therapy, we believe that it is important to critically examine the causal relationship of the reported event to the proposed etiology so that a balanced view of the cause and effect be arrived at. This is particularly the case in an uncontrolled setting where formal processes for documenting and reporting experimental therapy may not be in place.
Recent reviews of studies with one of the most widely used helminths, the anthropophilic hookworm, Necator americanus, have shown this helminth to be safe and well tolerated in hundreds of individuals by numerous research groups across Australia, the US and Europe (1–3). In BMC Pulmonary Medicine (4), Zeynalyan and colleagues report rapidly progressive respiratory failure in a patient with significant comorbidities, including systemic sclerosis, interstitial lung disease and pulmonary hypertension after self-administration of N. americanus larvae that were purchased over the internet. Here we raise some concerns about this report
The effect of Holstein-Friesian genotype and feeding system on selected performance parameters of dairy cows on grass-based systems of milk production in Ireland
End of project reportThe overall objective of this project was to assess, the effect of strain of Holstein-Friesian dairy cow, pasture-based feed system (FS) and their interaction on animal performance in terms of milk productivity and lactation profile, body weight (BW), body condition score (BCS), feed intake and energy balance (EB), reproductive performance and overall economic profitability
Photometric Response Functions of the SDSS Imager
The monochromatic illumination system is constructed to carry out in situ
measurements of the response function of the mosaicked CCD imager used in the
Sloan Digital Sky Survey (SDSS). The system is outlined and the results of the
measurements, mostly during the first 6 years of the SDSS, are described. We
present the reference response functions for the five colour passbands derived
from these measurements, and discuss column to column variations and variations
in time, and also their effects on photometry. We also discuss the effect
arising from various, slightly different response functions of the associated
detector systems that were used to give SDSS photometry. We show that the
calibration procedures of SDSS remove these variations reasonably well with the
resulting final errors from variant response functions being unlikely to be
larger than 0.01 mag for g, r, i, and z bands over the entire duration of the
survey. The considerable aging effect is uncovered in the u band, the response
function showing a 30% decrease in the throughput in the short wavelength side
during the survey years, which potentially causes a systematic error in
photometry. The aging effect is consistent with variation of the instrumental
sensitivity in u-band, which is calibrated out. The expected colour variation
is consistent with measured colour variation in the catalog of repeated
photometry. The colour variation is delta (u-g) ~ 0.01 for most stars, and at
most delta (u-g) ~ 0.02 mag for those with extreme colours. We verified in the
final catalogue that no systematic variations in excess of 0.01 mag are
detected in the photometry which can be ascribed to aging and/or seasonal
effects except for the secular u-g colour variation for stars with extreme
colours.Comment: 54 pages, 18 figures, 7 tables, accepted for publication in A
Safety Considerations for Malaria Volunteer Infection Studies: A Mini-Review
Malaria clinical studies entailing the experimental infection of healthy volunteers with Plasmodium parasites by bites from infected mosquitos, injection of cryopreserved sporozoites, or injection of blood stage parasites provide valuable information for vaccine and drug development. Success of these studies depends on maintaining safety. In this mini-review, we discuss the safety risks and associated mitigation strategies of these three types of experimental malaria infection. We aim to inform researchers and regulators who are currently involved in or are planning to establish experimental malaria infection studies in endemic or non-endemic settings
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