MOESM2 of Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease

Additional file 2: Figure S1. (A) Plasma levels IL-18BPa and free circulating IL-18 in dengue patients at febrile and defeverscence phase. (B) Spearman correlation between IL-18 and IL-18BP among DWS and DWS+/SD. Levels of biomarkers were compared across the three patient groups and post hoc Mann–Whitney U tests were then performed for those biomarkers with a Kruskal–Wallis test P value of <0.05. A Spearman rank test was used to compare the correlation between two continuous variables. ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05

Incidence of anaemia, iron deficiency and iron deficiency anaemia.

<p>Incidence of anaemia, iron deficiency and iron deficiency anaemia.</p

Comparison of Reference Intervals of various populations.

<p>Gaza Strip (N = 50,127)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091968#pone.0091968-Sirdah1" target="_blank">[1]</a>.</p><p>Germany(N = 2967)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091968#pone.0091968-Ittermann1" target="_blank">[6]</a>.</p><p>US (N = 7664) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091968#pone.0091968-Beutler1" target="_blank">[4]</a>, (N = not available)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091968#pone.0091968-NHANES1" target="_blank">[5]</a>, (N = not available)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091968#pone.0091968-Hsieh1" target="_blank">[8]</a>.</p><p>UK (N = 700)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091968#pone.0091968-OseiBimpong1" target="_blank">[2]</a>.</p><p>South India(N = 500)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091968#pone.0091968-Subhashree1" target="_blank">[3]</a>.</p><p>Ghana (N = 691)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091968#pone.0091968-Dosoo1" target="_blank">[7]</a>.</p>a<p>19–45 years old.</p>b<p>>45 years old.</p>c<p>20–81 years old.</p>d<p>50–59 years old.</p>e<p>60–69 years old.</p>f<p>19–65 years old.</p>g<p>66 years and above.</p>h<p>16–91 years.</p>i<p>18–70 years.</p>j<p>18–59 years.</p><p>W = White B = Black.</p

Reference intervals from this study compared with previous study.

<p>M =  male, F =  female.</p><p>* Median, range 2.5-97.5 centiles.</p><p>** Ferritin ng/mL<13 excluded.</p>§<p>Reference interval  =  mean±SD.</p

Clinical symptoms of the study population.

<p>*indicates the symptoms/manifestations considered as warning signs by the WHO 2009 dengue classification. Detailed clinical symptoms (in percentages) by dengue classification are given in the table.</p

HLA Allele Frequencies in the Study Population.

<p>(A) HLA-A alleles in the total study population; (B) HLA-B alleles in the total study population.</p

HLA association with dengue of the study population.

<p>HLA association with dengue of the study population.</p

Significant cytokines at different phases of illness when compared to the controls.

<p>Significant cytokines at different phases of illness when compared to the controls.</p

Clinical profile of study population.

<p>Scatter plot of the study population’s (A) vital signs (temperature, systolic, diastolic and pulse rate); (B) blood profile (hemoglobin, hematocrit, platelet, prothrombin time, activated partial thromboplastin time); (C) white blood cell profile (total white blood cells, lymphocytes, neutrophils and monocytes); (D) liver profile (total protein, total billirubin, albumin, globulin, alkaline phosphatase, alanine transaminase, aspartate aminotransferase) and (E) kidney profile (urea, sodium, potassium, chloride and creatinine) over days of illness. The LOWESS curves follows the general trend of the observed clinical parameter in dengue patients without (blue) and with (red) warning signs. Grey zone shows the reference range values.</p

Laboratory diagnostics confirmation of study population.

<p>ND: Non dengue; DwoWS: Dengue without warning signs; DwWS: Dengue with warning signs; SD: Severe dengue; IgM: Immunoglobulin M; NS1: Non-structural protein 1; HI: Hemagglutination Inhibiton Assay; +: combination.</p