Dysregulated Expression of MiR-19b, MiR-25, MiR-17, WT1, and CEBPA in Patients with Acute Myeloid Leukemia and Association with Graft versus Host Disease after Hematopoietic Stem Cell Transplantation

Objectives Acute myeloid leukemia (AML) is a blood malignancy characterized by the proliferation of aberrant cells in the bone marrow and blood that interfere with normal blood cells. We have investigated whether changes in the level of micro-ribonucleic acid (miR)-19b, miR-17, and miR-25, Wilms' tumor (WT1), and CCAAT enhancer-binding protein α (CEBPA) genes expression affect disease prognosis and clinical outcome in AML patients. Materials and Methods The expression level of miR-19-b, miR-17, and miR-25, as well as WT1 and CEBPA genes in a group of patients and controls as well as different risk groups (high, intermediate, and favorite risk), M3 versus non-M3, and graft-versus-host disease (GvHD) versus non-GvHD patients were assessed using a quantitative SYBR Green real-time polymerase chain reaction method. Results When compared with the baseline level at the period of diagnosis before chemotherapy, the expression of miR-19b and miR-17 in AML patients increased significantly after chemotherapy. The level of miR-19b and miR-25 expression in AML patients with M3 and non-M3 French–American–British subgroups differ significantly. MiR-19b and miR-25 expression was elevated in GvHD patients, while miR-19b and miR-25 expression was somewhat decreased in GvHD patients compared with non-GvHD patients, albeit the difference was not statistically significant. Also, patients with different cytogenetic aberrations had similar levels of miR-19-b and miR-25 expression. Conclusion MiR-19b, miR-17, and miR-25 are aberrantly expressed in AML patients' peripheral blood leukocytes, which may play a role in the development of acute GvHD following hematopoietic stem cell transplantation

Effects of co-incorporated ternary elements on biocorrosion stability, antibacterial efficacy, and cytotoxicity of plasma electrolytic oxidized titanium for implant dentistry

Funding Information: This research work has been supported with research grant (No.: 247383 ) by Materials and Energy Research Center (MERC), Karaj, Iran . Publisher Copyright: © 2021 Elsevier B.V.In this study, calcium, phosphorus, and copper co-incorporated titanium oxide (TiO2) layers were prepared on titanium substrates using the plasma electrolytic oxidation process. Thereafter, their features were studied to be used in dental implants. Surface characterization revealed that the addition of calcium and copper ions to the phosphate-based electrolyte led to the development of the ternary elements incorporated TiO2 layer with greater surface homogeneity, roughness, hydrophobicity, and growth rate. As well, electrochemical impedance spectroscopy proved that the placement of the ternary elements caused more compaction of the TiO2 layer by reducing inherent defects. Thus, the corrosion behavior of the TiO2 layer in artificial saliva solution has promoted, which consequently enhanced the corrosion potential by 187 mV and diminished the corrosion current density by one order of magnitude. Antibacterial assessment against Escherichia coli showed that incorporation of copper along with calcium and phosphorus significantly restored the bactericidal activity of the TiO2 layer. Furthermore, the integration, proliferation, and viability of MG-63 osteoblastic cells have considerably improved in the biological response to the calcium, phosphorus, and copper-containing layer.Peer reviewe