Adhesion molecules included in the CD11c⁺ T cell fraction after vaginal Chlamydia infection in mice.

<p>The frequency of the different subsets obtained from combining CCR10, CD8α, CD103 and NK1.1 expression is displayed for each group as a pie chart and as a complementary bar graph. Gating strategy was performed as described in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154253#pone.0154253.g001" target="_blank">Fig 1</a></b> and in Materials and Methods. The frequency of CD11c positive cells in the T cell fraction as the mean ± SD is shown as a white number in the center of the pie chart for each group in blood (top) and genital tract (GT, bottom). Each colored portion of a pie chart indicates the percentage of a specific subset detailed in the bar chart below. The arcs around the pie show the molecule or combination of molecules to which those proportions correspond (see color legend indicating CCR10, CD8α, CD103 and NK1.1). *Indicates p<0.05 by Student’s t test analyses only for values >5% of the total CD11c⁺ T cells in vaginally (VAG)-infected mice seven days after infection (n = 4) compared to control (n = 3) animals.</p

CD11c expression in circulating T cells from healthy young women.

<p>CD11c⁺ cells were analyzed by flow cytometry in the CD4⁺ CCR7⁺/^- and CD4^- CCR7⁺/^- CD3⁺ T cell subsets. Gating strategy consisted on the following consecutive gates: lymphocytes, singlets, live CD3⁺ T cells, CD4⁺ or CD4^- T cells, CCR7⁺ or CCR7^- and lastly CD11c⁺. Each bar represents the mean ± SD of normal donors (n = 13). Data were analyzed by Kruskall-Wallis test with Bonferroni post-test correction.</p

Frequency of adhesion molecules in CD38⁺ HLA-DR⁺ T_EM cells from different conditions affecting women.

<p>Percentages of the expression of chemokine receptors in CD38⁺ HLA-DR⁺ (<b>a</b>) CD4⁺ effector memory T (T_EM) cells and (<b>b</b>) CD8⁺ T_EM cells determined by flow cytometry are shown for normal donors (ND) and the different groups of patients. Percentages of the expression of integrins and other adhesion molecules in CD38⁺ HLA-DR⁺ (<b>c</b>) CD4⁺ T_EM and (<b>d</b>) CD8⁺ T_EM cells determined by flow cytometry are shown for ND and the different groups of patients. General gating strategy is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0156605#pone.0156605.g003" target="_blank">Fig 3</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0156605#pone.0156605.s002" target="_blank">S2 Fig</a>. Each bar represents the median ± interquartile range of healthy young women (ND; white bars, n = 13), women with psoriasis (PS; grey bars, n = 5), ulcerative colitis (UC; checkered bars, n = 4) and bacterial vaginosis (BV; dark bars, n = 5). P values indicate: *<0.05; **<0.01.</p

Gating strategy and representative plots of adhesion molecule analysis in circulating T_EM cells from women.

<p>The overall gating strategy for a representative single normal donor is shown. (<b>a</b>) General gating strategy for effector memory T (T_EM) cells consist of the following consecutive gates: lymphocytes, singlets and live CD3⁺ T cells (top row); CD4⁺ and CD4^- (putative CD8⁺) T cells, and the effector CCR7^- fraction for each of these subsets (bottom row). (<b>b</b>) Representative plots of molecules analyzed in T_EM cells in one of the panels are shown: activated CD38 and/or HLA-DR T_EM cells, expression of CCR5 and CCR2, expression of CXCR6, and expression of CXCR3 and CD11c for CD4⁺ T_EM cells (top row) and CD8⁺ T_EM cells (bottom row). Isotype controls are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0156605#pone.0156605.s003" target="_blank">S3 Fig</a>.</p