Decolorization of Disperse Orange 25 using Trichosanthes diocia proteins in the presence of redox mediator

AbstractThis paper describes a peroxidase from Trichosanthes diocia to study decolorization of Disperse Orange 25 under different experimental conditions like pH, temperature, time interval, enzyme concentration and in the presence of redox mediators 1-hydroxybenzotriazole (HOBT).T. diocia peroxidase showed remarkable decolorization of Disperse Orange 25 in the presence of 1-hydroxybenzotriazole.  At an enzyme concentration of 0.45 EUmL-1 the peroxidase could successfully decolorize the dye up to a maximum of 61.2% with 0.2 mM 1-hydroxybenzotriazole. Maximum decolorization was recorded at a temperature of 40°C and at pH 4.0. The time for achieving maximum decolorization was 120min. Thus, the study suggests that T. diocia peroxidase could be a potential source for developing an inexpensive and efficient method for the treatment of recalcitrant Disperse Orange 25 dyes that are potentially toxic.  Key words: Disperse Orange 25; 1-hydroxybenzotriazole; Decolorization; Trichosanthes diocia Peroxidas

Immobilized Enzymes – Characteristics and Potential Applications in Synthetic Dye Color Removal

In recent times, enzymatic approaches have been used in treatment of colored wastewater/industrial effluents. Peroxidases are very useful in removal of recalcitrant toxic compounds or transforming them into innocuous products. Although much attention has been paid in the utilization of biocatalysts in several fields, their involvement in solving the environmental problems has gained support. Enzymes in soluble states have limitations of catalytic ability and stability. As the complexity of the effluents increases, the ability of the enzymes to execute its catalytic potential decreases. Therefore, one of the approaches to improve stability, catalytic ability, reusability and shelf life of enzymes is by immobilization. Work in the area of enzyme technology has provided significant clues that facilitate using enzymes optimally at large scale by cross-linking, entrapping and immobilizing. The current article presents an insight into the use of peroxidases immobilized on several different supports for the dye color removal of synthetic dyes as well as several different contaminants

Soluble Fraction of Trichosanthes diocia Peroxidase in Decolorization of Reactive Orange 15

Salt fractionated proteins from Trichosanthes diocia was used to study decolorization of Reactive Orange 15 under multifarious experimental conditions like pH, temperature, time interval, enzyme concentration and in the presence of redox mediator 1-hydroxybenzotriazole (HOBT)/ vanillin. T. diocia peroxidase showed extensive decolorization of Reactive Orange15 in the presence of 1-hydroxybenzotriazole (HOBT) / vanillin. The dye was decolorized effectively with HOBT and so further studies were performed in the presence of HOBT.  At an enzyme concentration of 0.45 EUmL-1 the peroxidase was able to remove the reactive dye up to a maximum of 94.6% with 1.0 mM 1-hydroxybenzotriazole. Maximum decolorization was achieved at a temperature of 40°C, pH 5.0 and with an incubation period of 90 min. Thus, the data indicates that T. diocia peroxidase could be a potential source for developing an inexpensive and efficient method for the treatment of recalcitrant Reactive Orange15 dyes that are potentially toxic. ---Department of Biochemistry (DST-FIST & UGC-SAP Supported), Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P. (India)---Please Cite This Article As: Farrukh Jamal, Tabish Qidwai and Prabhash K Pandey. 2010. Soluble Fraction of Trichosanthes diocia Peroxidase in Decolorization of Reactive Orange 15. J. Ecobiotechnol. 2(5):36-41.Â