Studies on germination and seedling vigour characters for genotype selection in nutmeg (Myristica fragrans Houtt.)

An experiment was conducted at Ratnagiri (Maharashtra) to study the regeneration ability and variability of 34 nutmeg (Myristica fragrans) seedling genotypes. The variations recorded for germination percentage, period required for germination and seedling growth parameters were significant. Higher magnitudes of genotypic coefficient of variation, phenotypic coefficient of variation, heritability and genetic advance on per cent mean basis were recorded for fresh seed weight, germination percentage, days for germination and root length. The selection of nutmeg genotypes at seedling stage on the basis of vigour of the characters such as number, length and breadth of leaves, collar thickness and petiole length would help to identify genotypes for propagation. Among the genotypes studied, N22, N24, N26, N29, N34, N37, N38, N42, N43, N51, N63 and N72 were promising. &nbsp

Studies on genotypic response of nutmeg to softwood grafting

An experiment was conducted to study the success rate of softwood grafting in 22 female, 12 hermaphrodite and S male nutmeg genotypes. The variation among genotypes for sprouting, survival and growth parameters was statistically significant. The magnitudes of GeV, pev, habitability and genetic advance were low for sprouting and survival of nutmeg grafts. The graft survival has strong negative correlation with leaf width. Maximum graft sprouting was associated with the faster production of new leaves with less breadth and longer petiole. The genotypes, N66, N24, N61, Nl, NS, N29, N38 and N49 gave the best response to grafting. &nbsp

Development of an integrated intraspecific map of chickpea (Cicer arietinum L.) using two recombinant inbred line populations

A composite intraspecific linkage map of chickpea was developed by integrating individual maps developed from two F8:9 RIL populations with one common parent. Different molecular markers viz. RAPD, ISSR, RGA, SSR and ASAP were analysed along with three yield related traits: double podding, seeds per pod and seed weight. A total of 273 markers and 186 RILs were used to generate the map with eight linkage groups at a LOD score of ≥3.0 and maximum recombination fraction of 0.4. The map spanned 739.6 cM with 230 markers at an average distance of 3.2 cM between markers. The predominantly used SSR markers facilitated identification of homologous linkage groups from the previously published interspecific linkage map of chickpea and confirmed conservation of the SSR markers across the two maps as well as the variation in terms of marker distance and order. The double podding gene was tagged by the markers NCPGR33 and UBC249z at 2.0 and 1.1 cM, respectively. Whereas, seeds per pod, was tagged by the markers TA2x and UBC465 at 0.1 and 1.8 cM, respectively. Eight QTLs were identified that influence seed weight. The joint map approach allowed mapping a large number of markers with a moderate coverage of the chickpea genome and few linkage gap

Sitting at the edge: How biomolecules use hydrophobicity to tune their interactions and function

Water near hydrophobic surfaces is like that at a liquid-vapor interface, where fluctuations in water density are substantially enhanced compared to that in bulk water. Here we use molecular simulations with specialized sampling techniques to show that water density fluctuations are similarly enhanced, even near hydrophobic surfaces of complex biomolecules, situating them at the edge of a dewetting transition. Consequently, water near these surfaces is sensitive to subtle changes in surface conformation, topology, and chemistry, any of which can tip the balance towards or away from the wet state, and thus significantly alter biomolecular interactions and function. Our work also resolves the long-standing puzzle of why some biological surfaces dewet and other seemingly similar surfaces do not.Comment: 12 pages, 4 figure

A new ATPG technique (MultiDetect) for testing of analog macros in mixed-signal circuits

A new test-set selection technique based on the frequency-domain testing of analog circuits is presented in this paper. We propose a new automatic test pattern generation (ATPG) method known as MultiDetect for testing linear time invariant (LTI) circuits. The proposed technique is best suited for use of existing building blocks in systems-on-chip for implementation of an on-chip test-signal generator and test-response analyzer. The generated test set with the MultiDetect method can effectively detect and diagnose both soft and hard faults and does not require any precision analog signal sources or signal measurement circuits when implemented as built-in self-test (BIST). Testing of analog blocks based on circuit-transfer function makes our ATPG a general purpose method for all kinds of LTI circuits. A new novel test method causing the device under test to saturate or get out of saturation, to detect a fault with simple detection hardware, is also introduced in this paper. In our proposed novel test scheme, usage of a single sinusoid as a test signal when compared to multitone signal, and detection of faults with digital counting technique, facilitate the test implementation with simple BIST hardware and make testing more cost effective. The sinusoid is a very useful waveform for testing and analyzing LTI circuits. In the steady state, both the input and output of a stable LTI circuit are sinusoids of the same frequency. The relationship between the amplitudes and phase angles of the input and output sinusoids is frequency-dependent. Identification of a sinusoid that detects more faults results in an optimized test signal set. The technique used in the MultiDetect method for identification of a sinusoid results in an efficient compacted test set. The search for fault diagnosis is restricted to a limited set of faults, making diagnosis fast in the MultiDetect method. A methodology for test-set compaction of the MultiDetect technique is described and results of experiments on various test circuits are discussed. The proposed method is seen to be efficient for low-power applications. The experimental results show that the testing of LTI circuits using the MultiDetect technique for the benchmark circuits achieves the required fault coverage with much shorter testi-ng time

Single-molecule spectroscopy of cold denaturation and the temperature-induced collapse of unfolded proteins

Recent Förster resonance energy transfer (FRET) experiments show that heat-unfolded states of proteins become more compact with increasing temperature. At the same time, NMR results indicate that cold-denatured proteins are more expanded than heat-denatured proteins. To clarify the connection between these observations, we investigated the unfolded state of yeast frataxin, whose cold denaturation occurs at temperatures above 273 K, with single-molecule FRET. This method allows the unfolded state dimensions to be probed not only in the cold- and heat-denatured range but also in between, i.e., in the presence of folded protein, and can thus be used to link the two regimes directly. The results show a continuous compaction of unfolded frataxin from 274 to 320 K, with a slight re-expansion at higher temperatures. Cold- and heat-denatured states are thus essentially two sides of the same coin, and their behavior can be understood within the framework of the overall temperature dependence of the unfolded state dimensions