Overextension of Nonsetting Calcium Hydroxide in Endodontic Treatment: Literature Review and Case Report

Premixed non-setting calcium hydroxide (CaOH2) paste in pressure syringe system is commonly used in root canal therapy. The aim of this paper is to present a case involving an iatrogenic extrusion of the medicament during endodontic treatment and a literature review of similar reports. The present case demonstrates severe tissue necrosis and other deleterious effects following the extrusion of CaOH2 paste beyond root apex. A 21-year old female was referred for endodontic treatment of her maxillary left first premolar. After completion of the canal preparation, root canals were filled by premixed CaOH2 paste. In the second appointment, a gingival detachment and an irregular zone of necrosis adjacent to the tooth apex was observed. To treat this complication, a mucoperiosteal flap was raised and the extruded material and necrotic tissues were currettaged and the area sutured. The patient was prescribed antibiotics and followed up at 2 weeks, 6 months and 2 years. Two week follow up showed good soft tissue healing. Two years postoperatively, complete radiographic and clinical healing was observed. We can conclude that the application of CaOH2 should be carried out with care and preferably applied free hand or with a lentulo spiral rather than in a pressure syringe

Impact of β-Globin Mutations on Outcome of Matched Related Donor Hematopoietic Stem Cell Transplantation for Patients with β-Thalassemia Major

AbstractThe clinical outcome of hematopoietic stem cell transplantation (HSCT) for patients with β-thalassemia major (β-TM) can be affected by several factors. We investigated the influence of β-globin gene mutation in patients with β-TM on the clinical outcome of HSCT and conducted a prospective study of consecutive β-TM patients who underwent allogeneic HSCT at our center. Among 87 included patients, 62 (71%) had homozygous and 25 (29%) had compound heterozygous β-globin gene mutations. Intervening sequence II-1 appeared to be the most common mutation, with an occurrence rate of 33% in β-globin alleles. With a median follow-up of 12 months, the thalassemia-free survival and overall survival probabilities were 83% (standard error, 4%) and 90% (standard error, 3%), respectively. Overall survival was not found to be associated with the β-globin gene mutation status, but thalassemia-free survival was significantly improved in patients with homozygous mutations compared with patients with compound heterozygous mutations in univariate (91.2% versus 64.0%, P = .009) and multivariable (hazard ratio, 3.83; P = .014) analyses. This is the first report on the impact of β-globin mutation status on the outcome of β-TM after allogeneic HSCT and helps to better illustrate the course and prognosis of β-TM after transplantation

A frequency-translating hybrid architecture for wideband analog-to-digital converters

Many emerging applications call for wideband analog-to-digital converters and some require medium-to-high resolution. Incorporating such ADCs allows for shifting as much of the signal processing tasks as possible to the digital domain, where more flexible and programmable circuits are available. However, realizing such ADCs with the existing single stage architectures is very challenging. Therefore, parallel ADC architectures such as time-interleaved structures are used. Unfortunately, such architectures require high-speed high-precision sample-and-hold (S/H) stages that are challenging to implement. In this thesis, a parallel ADC architecture, namely, the frequency-translating hybrid ADC (FTH-ADC) is proposed to increase the conversion speed of the ADCs, which is also suitable for applications requiring medium-to-high resolution ADCs. This architecture addresses the sampling problem by sampling on narrowband baseband subchannels, i.e., sampling is accomplished after splitting the wideband input signals into narrower subbands and frequency-translating them into baseband where identical narrowband baseband S/Hs can be used. Therefore, lower-speed, lower-precision S/Hs are required and single-chip CMOS implementation of the entire ADC is possible. A proof of concept board-level implementation of the FTH-ADC is used to analyze the effects of major analog non-idealities and errors. Error measurement and compensation methods are presented. Using four 8-bit, 100 MHz subband ADCs, four 25 MHz Butterworth filters, two 64-tap FIR reconstruction filters, and four 10-tap FIR compensation filters, a total system with an effective sample rate of 200 MHz is implemented with an effective number of bits of at least 7 bits over the entire 100 MHz input bandwidth. In addition, one path of an 8-GHz, 4-bit, FTH-ADC system, including a highly-linear mixer and a 5th-order, 1 GHz, Butterworth Gm-C filter, is implemented in a 90 nm CMOS technology. Followed by a 4-bit, 4-GHz subband ADC, the blocks consume a total power of 52 mW from a 1.2 V supply, and occupy an area of 0.05 mm2. The mixer-filter has a THD ≤ 5% (26 dB) over its full 1 GHz bandwidth and provides a signal with a voltage swing of 350 mVpp for the subsequent ADC stage.Applied Science, Faculty ofElectrical and Computer Engineering, Department ofGraduat

Focused ultrasound-mediated bbb disruption is associated with an increase in activation of AKT: experimental study in rats

Abstract Background The Blood Brain Barrier (BBB) maintains the homeostasis of central nervous system by preventing the free passage of macromolecules from the systemic circulation into the brain. This normal physiological function of the BBB presents a challenge for delivery of therapeutic compounds into the brain. Recent studies have shown that the application of focused ultrasound together with ultrasound contrast agent (microbubbles) temporarily increases the permeability of the BBB. This effect is associated with breakdown of tight junctions, the structures that regulate the paracellular permeability of the endothelial cell layer. The influence of this ultrasound effect on the activation of intracellular signaling proteins is currently not well understood. Therefore, the aim of this study was to investigate the activation of cell survival signaling molecules in response to ultrasound-mediated BBB opening; Methods The BBB was disrupted in two four-spot lines (1-1.5 mm spacing) along the right hemisphere of rat brain with ultrasound beams (0.3 MPa, 120 s, 10 ms bursts, repetition frequency = 1 Hz) in the presence Definity microbubbles. Contrast-enhanced MRI images were acquired to assess the extent of BBB opening upon which the animals were sacrificed and the brains removed and processed for biochemical and immunohistochemical analyses; Results Immunoblotting of sonicated brain lysates resolved by SDS-PAGE demonstrated an increase in phosphorylation of Akt and its downstream signaling molecule, GSK3β, while the phosphorylation of MAPK remained unchanged. The elevated levels of pAkt and pGSK3β are still evident after 24 hours post-sonication, a time point where the integrity of the BBB is known to be re-established. Furthermore, immunofluoresence staining localized this increase in pAkt and pGSK3β levels to neuronal cells flanking the region of the disrupted BBB; Conclusions Our data demonstrates that ultrasound-mediated BBB disruption causes an activation of the Akt signaling pathway in neuronal cells surrounding the disrupted BBB

Contact-less Assessment of In-vivo Body Signals Using Microwave Doppler Radar

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