Breadth of peptides recognized following vaccination with Ad35-GRIN and Ad35-ENV.

<p>Breadth of peptides recognized following vaccination with Ad35-GRIN and Ad35-ENV.</p

The effect of vaccine-induced CD8 responses targeting conserved regions on <i>in vitro</i> virus inhibition.

<p>A) box whisker plot comparing the average capacity to inhibit virus replication when targeted sequence is conserved, a Mann-Whitney test for unmatched pairs was used to determine that virus inhibition was significantly higher when vaccines recognized a putative epitope conserved within the relevant virus B) a box whisker plot illustrating that vaccines inhibiting viruses to highest levels recognize epitopes significantly more conserved than those inhibited to lesser levels (Mann Whitney t-test). C) Linear regression analysis relating average conservation of putative epitopes targeted to average inhibition of individual viruses top, IIIB virus is encircled. There is a slight trend towards inhibition of viruses where targeted putative epitopes were more conserved, IIIB is encircled as an outlier (left panel). Where IIIB is excluded (right panel) trend becomes more pronounced and approaches significance.</p

Reactive peptides inducing responses in CD8 populations ascertained through ICS and ELISpot using expanded CD8 populations, where possible peptides are associated with predefined optimal epitopes using vaccinee HLA.

<p>Bold sequences appear more than once and indicate a preferential immune targeting.</p

Breadth of insert-specific T-cell responses.

<p>Elispot peptide matrices were used to determine the number of possible epitopes recognized by vaccinees. Vaccinees (classified by Volunteer IDs) are stratified by dose and vaccine regimen.</p

Total IFN-γ ELISPOT Response Rates and Magnitude.

<p>Responses at baseline (BL), 2 weeks after <i>prime (Prm)</i> and 2 weeks after boost (Bst) are shown. The y-axis shows the SFC/10⁶ PBMC on a half-log scale. All responses are background-subtracted. Mean responses <1 were set to 1. Black dots: response below the cut-off as defined in the materials and methods to any of the 12 MAG and GRIN-Env peptide pools; red circles: response above the cut-off to any of the 12 peptide pools. For the vaccine groups, the overlaid box plot summarizes the positive responses (i.e., the median, 1st and 3rd quartiles and minimum/maximum). The x-axis shows the % responders and number of responders out of the total tested at each time point. The placebo (Pbo) responses are combined in the far right box plot.</p

CONSORT Diagram.

<p>CONSORT Diagram.</p

Baseline Demographics.

<p>Baseline Demographics.</p

Env Antibody Titers.

<p>Panel A) HIV-Env subtype A (UG37) and panel B) HIV-Env subtype B (6101) specific IgG antibody titers were assessed at baseline, post prime and post boost. Black and Red arrows indicate vaccination times for Groups 1–3 and Groups 4–5, respectively.</p

Maximum Severity of Adverse Events (AE).

<p><b>A</b>. Local Solicited AE over 7 days, <b>B</b>. Systemic Solicited AE over 7 days, <b>C</b>. Unsolicited AE over 28 days.</p

VIA breadth.

<p>VIA was assessed at baseline, 2–4 weeks post HIVMAG (x3) and at 2 weeks post Ad35-GRIN/Env boost in Groups 1–3. Each symbol represents an individual and the bar shows the mean number of viruses inhibited out of a total of 8 viruses in the panel.</p