Massive burn > 90% of body surface – case report

Severe burns are life-threatening injuries very difficult to heal. Multidisciplinary treatment is necessary due to complicated pathophysiological cascades induced by massive tissue destruction. Children and elderly people are especially endangered in being burnt. We present case of 73 years old woman with massive burn injury of >90% body surface

The use of dynamic sentinel node biopsy in case of penile cancer. A case report

Penile cancer is a rare disease with prevalence of approximately 1/100000 man in Europe. Well documented risk factors for a penile cancer are HPV Infection, phimosis, and chronic inflammation. Penile cancer is a curable condition when diagnosed and treated promptly. The invasion of lymphatic system is one of the most important matters affecting long term survival. Proper evaluation and treatment of lymph nodes is a main goal when dealing with this condition. In patients with palpable lymph nodes lymphadenectomy is method of choice. When dealing with unpalpable lymph nodes and increased risk of micro-metastatic disease, invasive nodal staging is recommended. For this staging we can choose from Modified inguinal lymphadenectomy and Dynamic Sentinel Node Biopsy In this paper we would like to present a case report of patient with penile cancer, where due to TNM staging, Dynamic Sentinel Node Biopsy was implemented

The influence of varicocelectomy on semen parameters and fertility

Introduction. Varicocele is an abnormal dilation of the spermatic veins within the pampiniform plexus of the spermatic cord. This pathology is diagnosed in 25% of infertile men. There is some data that confirms varicocelectomy improves semen parameters and fertility potential in men with clinically diagnosed varicocele and impaired semen parameters. The aim of our study was to determine the influence of laparoscopic varicocelectomy on semen parameters and evaluation of spontaneous pregnancy rate. Material and methods. We retrospectively reviewed the data of 60 consecutive patients subjected to laparoscopic varicocelectomy between November 2010 and December 2016 due to clinical varicocele, impaired semen parameters and infertility. Results. The average age was 31 +/- 7. Compared with baseline semen parameters, sperm count, sperm concentration, morphology and progressive motility at 3 and 6 months. We did not observe changes in semen volume and semen pH during follow-up. A total improvement in semen parameters was observed in 64% and 70% of patients after 3 and 6 months after surgery. In 4 patients deterioration of semen parameters was noticed. Spontaneous pregnancy was achieved in 30% of couples. Two pregnancies were unsuccessful due to spontaneous abortion. A total of 16 children were born. The surgery- to- conception time was 7.7 +/- 7 months. Conclusions. Laparoscopic varicocelectomy in a patient who has suffered from infertility and confirmed semen parameters and fertility potential of men

Management of large staghorn calculi – PCN Approach

Urolithiasis is common condition affecting more than 10 % of well-developed country’s population. Formation of the stones may have few different causes. Stones can be classified by size, location, etiology, composition, and risk of recurrence. Evaluation of the stones should consist of imaging : X-Ray, CT, USG. When dealing with staghorn calculi PCN approach should be method of choice. This procedure is relatively safe and effective. In this paper we would like to present a case report of large and very hard complete staghorn calculi which was managed by the PCN

SARS-CoV-2 Genetic Variability and Non-Specific Immunity Associated with the Use of Different BCG Strains—A Molecular and Clinical Approach

The effect of BCG vaccination against tuberculosis on the reduction in COVID-19 infection is related to the effect of the BCG vaccine on the immunomodulation of non-specific immunity. In the early stages of the pandemic, countries with universal BCG vaccination programs registered a low number of new cases of COVID-19, with the situation now reversed, as exemplified by India. The high genetic variability of SARS-CoV-2, a known characteristic of RNA viruses, causing the occurrence of SARS-CoV-2 variants may have led to the virus adapting to overcome the initial immune protection. The strains from the United Kingdom (B1.1.7), Brazil (B1.1.28 and B1.1.33), South Africa (B.1.351), and India (B.1.617) are characterized by a greater ability to spread in the environment, in comparison with the original infectious agent of SARS-CoV-2. It should be remembered that the large variation in the genetic makeup of SARS-CoV-2 may result in future changes in its pathogenicity, immunogenicity and antigenicity, and therefore it is necessary to carefully study the mutations occurring within the virus to determine whether the current vaccines will remain effective. However, most studies show that monoclonal antibodies produced after vaccination against COVID-19 are effective against the newly developed variants

New Molecular Markers Involved in Regulation of Ovarian Granulosa Cell Morphogenesis, Development and Differentiation during Short-Term Primary In Vitro Culture—Transcriptomic and Histochemical Study Based on Ovaries and Individual Separated Follicles

Nowadays, science has a lot of knowledge about the physiology of ovarian processes, especially folliculogenesis, hormone production and ovulation. However, the molecular basis for these processes remains largely undiscovered. The cell layer surrounding the growing oocyte—granulosa cells—are characterized by high physiological capabilities (e.g., proliferation, differentiation) and potential for growth in primary cultures, which predisposes them for analysis in the context of possible application of their cultures in advanced methods of assisted reproduction. In this study, we have used standard molecular approaches to analyze markers of these processes in primarily in vitro cultured porcine granulosa, subjected to conditions usually applied to cultures of similar cells. The material for our research came from commercially slaughtered pigs. The cells were obtained by enzymatic digestion of tissues and in vitro culture in appropriate conditions. The obtained genetic material (RNA) was collected at specific time intervals (0 h—before culture; reference, 48, 98, 144 h) and then analyzed using expression microarrays. Genes that showed a fold change greater than |2| and an adjusted p value lower than 0.05 were described as differentially expressed. Three groups of genes: “Cell morphogenesis”, “cell differentiation” and “cell development” were analyzed. From 265 differently expressed genes that belong to chosen ontology groups we have selected DAPL1, CXCL10, NEBL, IHH, TGFBR3, SCUBE1, DAB1, ITM2A, MCOLN3, IGF1 which are most downregulated and PDPN, CAV1, TMOD1, TAGLN, IGFBP5, ITGB3, LAMB1, FN1, ITGA2, POSTN genes whose expression is upregulated through the time of culture, on which we focused in downstream analysis. The results were also validated using RT-qPCR. The aim of our work was to conduct primary in vitro culture of granulosa cells, as well as to analyze the expression of gene groups in relation to the proliferation of follicular granulosa cells in the model of primary culture in real time. This knowledge should provide us with a molecular insight into the processes occurring during the in vitro cultures of porcine granulosa cells, serving as a basic molecular entry on the extent of the loss of their physiological properties, as well as gain of new, culture-specific traits

New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes

Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in ‘cell development’, ‘cell growth’, ‘cell differentiation’ and ‘cell maturation’ processes have been identified in porcine oocytes. The aim of the study was to assess the expression profile of genes in primary in vitro cultured oviductal epithelial cells (OECs), clustered in Gene Ontology groups which enveloped markers also identified in porcine oocytes. OECs (from 45 gilts) were surgically removed and cultured in vitro for ≤ 30 days, and then subjected to molecular analyses. The transcriptomic and proteomic profiles of cells cultured during 7, 15 and 30 days were investigated. Additionally, morphological/histochemical analyzes were performed. The results of genes expression profiles were validated after using RT-qPCR. The results showed a significant upregulation of UNC45B, NOX4, VLDLR, ITGB3, FMOD, SGCE, COL1A2, LOX, LIPG, THY1 and downregulation of SERPINB2, CD274, TXNIP, CELA1, DDX60, CRABP2, SLC5A1, IDO1, ANPEP, FST. Detailed knowledge of the molecular pathways occurring in the OECs and the gametes that contact them may contribute both to developments of basic science of physiology, and new possibilities in advanced biotechnology of assisted reproduction