Project Synthesis of N Acetyl L Histidine Ethyl Amide and Standard Operating Procedure for Fractional Collector

Photo oxidation of proteins leads to the formation of cross links of the amino acids in the proteins and leads to induction of cataracts, photo aging of the skin and in the Photodynamic therapy of tumors. Oxidation of proteins involves significant modification of particular amino acids residues with consequent photodynamic damage. Studies show that tryptophan, tyrosine and histidine side chains gives rise to semi stable peroxides on exposure to singlet oxygen these oxidized structures are studied with low temperature NMR. These peroxides or species derived from them have been postulated as key intermediates in subsequent reactions such as cross linking of oxidized amino acids with unmodified residues. It is difficult to identify modified amino acids in photo dynamically treated proteins and to determine their positions/locations .It is tedious to isolate the cross linked amino acid moieties from proteins, hence low molecular weight histidine and imidazole derivatives have been used to study the detailed reaction mechanisms. photo oxidation of N acetyl histidine ethyl amide derivative of histidine in which the amine group and the carboxylic acid group have been converted to amide bonds .this conversion makes this amino acid a peptide resembling amino acid so that its photo oxidation mimics the photo oxidation of histidine moiety in proteins. Use of N-Benzoyl-L histidine as a model compound elucidates the chemical structure and mechanisms of the formation of His-His cross links .Benzoyl group protects the alpha amino group of histidine participation in the cross link reactions .Benzoyl absorbs strongly in UV hence permits the spectroscopic identification Rose Bengal is used as a photo sensitizer which sensitizes primarily by the singlet oxygen mechanism in the aqueous solution It causes extensive damage to the imidazole ring of Histidine by forming semi stable peroxides which results in the formation of Hydrated Imidazolone with the second molecule of N acetyl histidine ethyl amide. The formation of this dimer shows the strong possibility of cross linking in the histidine residues in proteins. Lab synthesized N acetyl L histidine ethyl amide was analyzed by TLC, Melting point and NMR and declared that it was impure. Purification process was done through re-crystallization and Running the analyte down through silica column by gradient elution using three different solvents Hexanes, Ethyl acetate and Methanol. Synthesis of N acetyl L histidine ethyl amide meant to synthesize in lab Lab synthesis is done in two steps: Step1. Synthesizing N acetyl L histidine ethyl ester from L Histidine Step2. Synthesizing N acetyl L Histidine ethyl amide from N acetyl L histidine ethyl ester N acetyl L histidine ethyl ester is synthesized in lab on proceeding upon second step reconversion of N acetyl L histidine ethyl ester to L histidine was observed

Ca2+アンケージング反応を志向したEGTAユニットを有する二光子吸収発色団の設計と合成

内容の要約広島大学(Hiroshima University)博士(理学)Doctor of Sciencedoctora

Design and synthesis of a new chromophore, 2-(4-nitrophenyl)benzofuran, for two-photon uncaging using near-IR light

C. K. acknowledges the HPC resources of CINES and of IDRIS under the allocations 2014-[x2014080649] made by GENCI (Grand Equipement National de Calcul Intensif).International audienceA new chromophore, 2-(4-nitrophenyl)benzofuran (NPBF), was designed for two-photon (TP) uncaging using near-IR light. The TP absorption (TPA) cross-sections of the newly designed NPBF chromophore were determined to be 18 GM at 720 nm and 54 GM at 740 nm in DMSO. The TP uncaging reaction of a caged benzoate with the NPBF chromophore quantitatively produced benzoic acid with an efficiency (δu) of ∼5.0 GM at 740 nm. The TP fragmentation of an EGTA unit was observed with δu = 16 GM. This behavior makes the new chromophore a promising TP photoremovable protecting group for physiological studies

SPEED CONTROL OF INDUCTION MOTOR USING ADR CONTROLLER

The control of induction motors has been a primary concern of researchers. Proportional-integral- derivative (PID) control and direct torque control (DTC) have been proposed for induction motors [1-6]. However, the control of induction motors is still a challenging problem due to the following issues: 1) the dynamic system of an induction motor is highly nonlinear; 2) the rotor resistance varies because of heating. In this paper, a reduced-order active disturbance rejection control (ADRC) with reduced order extended state observer (RESO) is applied for induction motor control. The ADRC is employed for controlling a sli

Scholars Journal of Medical Case Reports ISSN 2347-9507 (Print) Congenital Rubella Syndrome with Basal ganglia calcification and Bilateral Nuclear Cataracts in a Neonate: A Rare Entity

Abstract: In-utero transmission of rubella virus during pregnancy leads to congenital Rubella syndrome (CRS), which is characterized by cataracts, deafness and sensorineural hearing loss. While the nuclear cataract is the most frequently occurring abnormality, basal ganglia calcification is a rarer manifestation. The Risk of fetal transmission of rubella virus is highest during the first 12 weeks of gestation and decreases afterwards. A 36weeks, 2600grams male neonate was born by vaginal delivery with normal APGARS. The neonate was admitted on third day of life with complaints of yellowish discoloration of the skin and sclera. On examination, the neonate had mild hypotonia, icterus up to the legs and systolic murmur on cardiac examination. Interestingly, the ophthalmological examination revealed bilateral nuclear cataracts. Chest radiograph showed cardiomegaly and ECHO revealed PDA (Patent Ductus Arteriosus). Hence, CRS was suspected and further evaluation was done. There was thrombocytopenia, mild unconjugated hyperbilirubinemia with mild elevation of transaminases. Another rare finding was basal ganglia calcification on neurosonogram. TORCH profile in both mother and baby showed elevated rubella IgM levels confirming CRS. The neonate received supportive and symptomatic treatment. We report a preterm, male appropriate for gestational age neonate with congenital rubella syndrome and its rare manifestation of basal ganglia calcification