TOPICAL APPLICATION OF EUGENIA CARYOPHYLLUS OIL AGAINST RINGWORM INFECTION OF HUMAN BEINGS

Objective: At least one million people are suffering from dermatophytosis in India. These mycoses although normally not lethal are unpleasant, frequent disease recurrence, and resistant dermatophytic strains cause considerable economic losses. During the antifungal evaluation of essential oil of Eugenia caryophyllus, all concentrations were found to be an excellent inhibitor against selected fungi as compared to standard antifungal drugs griseofulvin, ketoconazole, and itraconazole. The present work deals with the preparation of an ointment from E. caryophyllus oil for the treatment of ringworm infection in human beings. Materials and Methods: Due to these potent fungicidal properties, an ointment of E. caryophyllus was prepared and topically applied on tinea patients attending the outpatient Department of Skin, Venereology, and Leprology, SMS Hospital, Jaipur. Patients were diagnosed as tinea corporis, tinea capitis, tinea manuum, and tinea barbae. The medication was done twice a day for 3 weeks as advised by the skin specialist. Results: All patients showed positive potassium hydroxide (KOH) results at the beginning of the trial. After the 2nd week of treatment, every patient was KOH negative and remained negative when re-examined after one month of treatment. All patients were completely cured within 3 weeks of the treatment. Conclusions: Ointment showed excellent results, found cheaper substitutes to cure the disease without any adverse side effect. The present study offers a high possibility of complete cure of tinea infection and suggesting its uses as raw material by pharmaceutical industries for the development of antidermatophytic drug in prevailing conditions where dermatophytes are becoming resistant against popular antifungals

SCREENING OF THYMUS VULGARIS ESSENTIAL OIL AGAINST FUNGI CAUSING DERMATOPHYTOSIS IN HUMAN BEINGS

Objective: The study was designed to determined antidermatophytic activity of Thymus vulgaris essential oil against fungi causing superficial skin infections in human and animal.Methods: Minimum inhibitory concentration (MIC) of essential oil was screened against selected pathogenic fungi namely Trichophyton rubrum (MTCC 296), T. mentagrophytes (MTCC 7687), Microsporum gypseum (MTCC 4524), M. fulvum(MTCC2837), T. soudanense and T. interdigitale through semi solid agar antifungal susceptibility testing method(SAAS). Minimum fungicidal concentration (MFC) was also determined by modified semi solid agar antifungal susceptibility method. T. soudanense and T. interdigitalis were isolated through TO. KA. VA hair bating technique from animal habitats soil of Jaipur district.Results: T. vulgaris essential oil exhibited excellent antideramtophytic activity against all selected dermatophytes. Minimum inhibitory concentration was ranged from 0.020±0.000μl/ml to 0.1±0.033 μl/ml. MFC were found little higher than MIC (0.02±0.000μl/ml to.2±0.000μl/ml). M. gypseum was found to be most susceptible fungus as compared to other test fungi.Conclusion: T. vulgaris was found to be most effective fungicidal agent against human pathogenic fungi. Present findings provide for a rationale basis of a possible utilization of this oil in fields requiring safe and cheap compounds with antiseptic and preservative properties, such as cosmetic, pharmaceutical and food industries

SCREENING OF LAWSONIA INERMIS ESSENTIAL OIL AGAINST FUNGI CAUSING DERMATOPHYTIC INFECTION IN HUMAN

ABSTRACTObjective: Lawsonia inermis belonging to family Lythreace commonly known as henna has been used in traditional herbal medicine from age. Thisstudy deals with the extraction of henna essential oil, separation of essential oil fractions and antidermatophytic behavior of oil and their fractions.Methods: In present investigation, essential oil obtained from the leaves of Lawsonia through hydrodistillation method was screened for theirantidermatophytic activity against selected dermatophytes through disc diffusion technique and minimal inhibitory concentration (MIC) by semisolidagar susceptibility testing methods. Lawsonia oil was further subjected in buchii glass oven equipment for the separation of the different fraction atdifferent temperature interval. These fractions were labeled as LAI, LAII, LAIII, LAIV, and LAV.Results: MIC of Lawsonia essential oil was ranging from 0.025 to 1.5 µl/ml against selected dermatophytes and other related fungi. MIC of thesefractions were also studied which were ranging from 0.3 to <4 µl/ml. Trichophyton rubrum was found to be most susceptible fungus and Candidaalbicans was most resistant strain. Among all fractions studied LAII was found to be the most effective fraction. Fraction LA was discarded because ofvery less amount which could not be applied for MIC.Conclusion: The L. inermis demonstrating broad spectra of activity may help to discover new antibiotics that could serve as selective agents for themaintenance of animal or human health and provide biochemical tools for the study of infectious diseases. This versatile medicinal plant is the uniquesource of various types of chemical compounds, which are responsible of the various activities of the plant.Keywords: Essential oil, Dermatophytes, Trichophyton rubrum, Minimal inhibitory concentration.

Automatic Blood Vessel Extraction of Fundus Images Employing Fuzzy Approach

Diabetic Retinopathy is a retinal vascular disease that is characterized by progressive deterioration of blood vessels in the retina and is distinguished by the appearance of different types of clinical lesions like microaneurysms, hemorrhages, exudates etc. Automated detection of the lesions plays significant role for early diagnosis by enabling medication for the treatment of severe eye diseases preventing visual loss. Extraction of blood vessels can facilitate ophthalmic services by automating computer aided screening of fundus images. This paper presents blood vessel extraction algorithms with ensemble of pre-processing and post-processing steps which enhance the image quality for better analysis of retinal images for automated detection. Extensive performance based evaluation of the proposed approaches is done over four databases on the basis of statistical parameters. Comparison of both blood vessel extraction techniques on different databases reveals that fuzzy based approach gives better results as compared to Kirsch’s based algorithm. The results obtained from this study reveal that 89% average accuracy is offered by the proposed MBVEKA and 98% for proposed BVEFA

Retinal blood vessel localization to expedite PDR diagnosis

Ophthalmologist relies on the retinal fundus image segmentation for accurate diagnosis of Diabetic Retinopathy caused due to prolonged deterioration in retinal blood vessels. Blood vessel and optical disc localization determines the vascular alterations helpful in identifying retinal diseases with accurate identification of pathologies like microaneurysms and exudates. This work comprises evaluation of proposed Optical Disc Segmentation and blood vessel localization techniques followed by a statistical analysis using three fractal dimensions; box count, information and correlation. Fractal dimensions explored are beneficial for Proliferative Diabetic Retinopathy (PDR) diagnosis as its value for vascular structures increases with increasing level of PDR. Two benchmark fundus image databases, DRIVE and STARE were evaluated by utilizing shape and fractal features for performance validation and average accuracies of 96.79% and 95.68% were achieved for extracted blood vessels using proposed approach

Comparative study of enzyme dehydrogenase activity in Cicer arietinum and Arachis hypogea

Viability of seeds can be determined by the dehydrogenase activity of the constituent cells. Enzyme activity can be seen both inside and outside the cell. Dehydrogenase activity can be observed by the formation of formazan compound, a red dye, from 2, 3, 5, Triphenyl Tetrazolium Chloride (TTC). Dehydrogenase can catalyze the reaction. Initially red colour is observed in the seed tissues and gradually the whole solution turns red. Change in colour of the solution is observed with respect to time. A comparative study is conducted between Cicer arietinum(chick pea) and Arachis hypogea( ground nut). Higher enzyme content is reported in chickpea as the reaction is completed in 18 hours, as compared to groundnut where the reaction takes 48 hours to complete. It is seen that the content of enzyme and rapid leaching of formazan is responsible for the process

Recurrent madura foot without draining sinuses: a case report

Madura foot or mycetoma is a chronic granulomatous disease characterized by localized infection of subcutaneous tissues by actinomycetes or fungi. The recurrence rate for the disease if treated inadequately is very high. Recurrence presents with swelling and multiple discharging sinuses. This is an unusual presentation of the disease without discharging sinuses which is probable the first report of this kind in the literature. A 34 year old, male, presented with the painless, progressive swellings over right foot since 4 years. No sinuses or discharge could be found on skin surface. The postoperative recurrence rate is very high, and this can be local or distant at the regional lymph nodes. This could be due to the disease biology and behavior or inadequate surgical excision. Usually it presents with multiple sinus tracts, and granule. We reported a case with classical absence of sinus tracts in recurrent actinomycosis

SYNTHESIS, FREE RADICAL SCAVENGING AND DNA CLEAVAGE ACTIVITIES OF SOME NOVEL INDOLE DERIVATIVES

Objective: Synthesis of a series of novel indole derivatives (6a-h) by condensation of indolyl chalcones (5a-h) with thiobarbituric acid to evaluate free radical scavenging and DNA cleavage activity.Methods: The newly synthesized compounds were screened for free radical scavenging activity by DPPH method. The DNA cleavage activity of some indole derivatives was studied by agarose gel electrophoresis method. The structures of the synthesized compounds are assigned on the basis of elemental analysis, IR, 1HNMR, 13C NMR and mass spectral data.Results: Among the synthesized compounds the simple indole derivative (6a) has very good scavenging activity, chloro and fluoro substituted indole derivatives (6g), (6h) have shown moderate activities and methyl derivatives (6e), (6f) have shown least activity compare with the standard. All the tested compounds in the series have exhibited promising DNA cleavage activity.Conclusion: A series of novel indole derivatives were synthesized and evaluated for free radical scavenging and DNA cleavage activity. The compound (6g) was found most active among all the synthesized compounds.Â

A rare case of cervical fibroid presenting as retained placenta with postpartum hemorrhage

Fibroids during pregnancy are often asymptomatic but sometimes may lead to various complications and unusual presentations. We are reporting an unusual case of cervical fibroid during pregnancy that presented as retained placenta with postpartum hemorrhage following a full term normal vaginal delivery