2 research outputs found
<b>Effect of <i>N<sup>6</sup></i>-Benzyl amino purine and Naphthalene acetic acid on camptothecin production through <i>in vitro</i> propagation of </b> <b><i>Ophiorrhiza</i></b><b><i> rugosa </i>Wall.</b><b> var.<i> decumbens</i> (Gardn. ex Thw.) Deb & Mondal </b> <b> </b>
405-409In
vitro production of camptothecin (CPT) was achieved by the establishment of multiple shoot and root cultures of Ophiorrhiza rugosa
Wall. var. decumbens (Gardn. ex Thw.) Deb & Mondal. The maximum multiple
shoots were obtained in Murashige and Skoog (MS) medium supplemented with 5mg/l
N6-benzyl amino purine (BA) along
with 0.5mg/l naphthalene acetic acid (NAA). Optimal root generation
was observed in Murashige and Skoog medium with 2mg/l NAA and 0.05mg/l BA. However, CPT
content found to be elevated in shoot and root cultures where growth hormones
imposed growth inhibition. The maximum amount of CPT in multiple shoots was
0.039% while shoots of plant grown in the field showed 0.002 per cent. In root
cultures, CPT content was 0.065% whereas roots of intact plant showed only 0.024
per cent