Ischemic preconditioning does not acutely improve load-insensitive parameters of contractility in in vivo stunned porcine myocardium

AbstractObjective: Ischemic preconditioning has been shown to have no beneficial effect on segment shortening in in vivo regionally stunned myocardium. The purpose of this study was to determine whether ischemic preconditioning improves the recovery of postischemic ventricular function when contractility is assessed by load-insensitive measurements including end-systolic pressure length relations, preload recruitable stroke work, and preload recruitable stroke work area in in vivo regionally stunned porcine myocardium. Methods: Open chest, pentobarbital-anesthetized pigs were used. Regional ventricular function was monitored by measurements of segment shortening, stroke work, end systolic pressure length relations, preload recruitable stroke work, and preload recruitable stroke work area. The control group was submitted to 15 minutes of left anterior descending coronary artery occlusion and 3 hours of reperfusion. The preconditioned group underwent 2 cycles of 5-minute left anterior descending coronary artery occlusion and 10-minute reperfusion before 15 minutes of occlusion. Results: There was no infarct in either group. The preconditioning protocol significantly depressed preischemic segment shortening but not regional stroke work. Ischemic preconditioning had no significant beneficial effect on regional stroke work, end-systolic pressure length relations, preload recruitable stroke work, or preload recruitable stroke work area. Conclusions: These results confirm that ischemic preconditioning does not ameliorate in vivo porcine myocardial stunning and indicate that ischemic preconditioning may have a limited cardioprotective role during cardiac operation. (J Thorac Cardiovasc Surg 1999;117:810-7

A Novel Approach to ICD Lead Revision in a Patient with Extensive Vascular Thrombosis

Chronic extensive thrombosis of the venous system is a commonly encountered problem in end stage renal disease (ESRD) patients undergoing transvenous device implantation, lead extraction or lead revision. We describe a case of an ESRD patient with an implantable cardioverter defibrillator (ICD) that failed to deliver therapy due to lead fracture. Patient needed revision of the ICD lead system, but had extensive axillary-subclavian-superior vena cava occlusion. Patient refused a thoracotomy approach as well as lead extraction as he had a complicated course of lead extraction in the past. We successfully improvised a novel technique to revise the ICD system

KEEFEKTIFAN METODE LATIHAN TERBIMBING DENGAN MEDIA GAMBAR SERI PADA KETERAMPILAN MENULIS CERITA FABEL PADA KELAS VII SMP NEGERI 1 WONOMULYO

Penelitian ini adalah penelitian eksperimen yang bertujuan untuk mengetahui Keefektifan Metode Latihan Terbimbing Dengan Media Gambar Seri Pada Keterampilan Menulis Cerita Fabel . Subjek dalam penelitian ini adalah siswa kelas VII B untuk kelas eksperimen berjumlah 32 orang dan kelas VII C untuk kelas control berjumlah 32 orang SMP Negeri 1 Wonomulyo. Instrumen pada penelitian ini yaitu lembar observasi aktivitas siswa, angket respon siswa, dan tes keterampilan menulis cerita fabel . data yang terkumpul dianalisis dengan menggunakan  analisis statistic deskriptif dan inferensial. Dari Hasil analisis statisti, diperoleh nilai rata rata pretes pada kelas eksperimen yaitu  48,91, dan kelas control yaitu 50.63, sedangkan nilai rata rata posttest pada kelas eksperimen 73,97 dan kelas control yaitu 51,56. Dilihat dari aktifitas siswa diperoleh rata rata persentase sebesar 85% dan respon siswa setelah pembelajaran sebesar 90% Sehingga disimpulkan bahwa metode pembelajaran Latihan terbimbing dengan media gambar seri  berpengaruh atau efektif terhadap hasil belajar peserta didik

Profound cardioprotection with chloramphenicol succinate in the swine model of myocardial ischemia-reperfusion injury

Background-: Emerging evidence suggests that adaptive induction of autophagy (the cellular process responsible for the degradation and recycling of proteins and organelles) may confer a cardioprotective phenotype and represent a novel strategy to limit ischemia-reperfusion injury. Our aim was to test this paradigm in a clinically relevant, large animal model of acute myocardial infarction. Methods and results-: Anesthetized pigs underwent 45 minutes of coronary artery occlusion and 3 hours of reperfusion. In the first component of the study, pigs received chloramphenicol succinate (CAPS) (an agent that purportedly upregulates autophagy; 20 mg/kg) or saline at 10 minutes before ischemia. Infarct size was delineated by tetrazolium staining and expressed as a % of the at-risk myocardium. In separate animals, myocardial samples were harvested at baseline and 10 minutes following CAPS treatment and assayed (by immunoblotting) for 2 proteins involved in autophagosome formation: Beclin-1 and microtubule-associated protein light chain 3-II. To investigate whether the efficacy of CAPS was maintained with delayed treatment, additional pigs received CAPS (20 mg/kg) at 30 minutes after occlusion. Expression of Beclin-1 and microtubule-associated protein light chain 3-II, as well as infarct size, were assessed at end-reperfusion. CAPS was cardioprotective: infarct size was 25±5 and 41±4%, respectively, in the CAPS-pretreated and CAPS-delayed treatment groups versus 56±5% in saline controls (P\u3c0.01 and P\u3c0.05 versus control). Moreover, administration of CAPS was associated with increased expression of both proteins. Conclusion-: Our results demonstrate attenuation of ischemia-reperfusion injury with CAPS and are consistent with the concept that induction of autophagy may provide a novel strategy to confer cardioprotection. © 2010 American Heart Association, Inc