Detection of Shigella dysenteriae STX1 gene from Mazandaran province clinical samples by PCR-ELISA method

Background:Among enterobacteriaceae bacteria, Shigella dysenteriae produce a shiga protein toxin, and have cytotoxicity, enterotoxin and neurotoxin activity. The toxin causes diseases such as diarrhea, gastroenteritis, intravascular coagulation disorder. Today diarrhea is the most important challenge for the human health. The classic and conventional microbiological detection methods are sensitive and specificity, there is limitation. This study was designed to identify shigella dysenteriae in shortest time and the amount of toxin Stx1 with high sensitivity and specificity by using PCR-ELISA method. Method and material: The Stx1 sequence (490bp) as a target gene was amplified by Dig-dUTP labeled, then product was coating on microplate and by using anti antibody digoxigenin conjugated detection was done.  Also the specificity and sensitivity of method with clinical specimens examined. Results: Sensitivity detection of bacteria in the sample using genomic DNA for Shigella dysenteriae was 1/56pg and specificity of technique didn’t showed acceptable OD in other species of enterobacteriaceae family. ELISA for genomic DNA Up to dilution 0/156 pg had significant absorption. As well as from 70 clinical samples which was analyzed, 3 samples contained stx1 gene. Conclusion: Efficiency PCR-ELISA techniques showed that it was simple, faster, high specific and sensitive methods. This technique is more suitable than culture and PCR method also it was easily can applied in each medical laboratory

Factors that impact evaluation of left ventricular systolic parameters in myocardial perfusion gated SPECT with 16 frame and 8 frame acquisition models | [16 ve 8 frame görüntüleme modelli miyokard perfüzyon gated SPECT çalışmasında sol ventrikül sistolik parametrelerinin değerlendirilmesini etkileyen faktörler

Objective: Evaluating the effects of heart cavity volume, presence and absence of perfusion defect, gender and type of study (stress and rest) on the difference of systolic parameters of myocardial perfusion scan in 16 and 8 framing gated SPECT imaging. Methods: Cardiac gated SPECT in both 16 and 8 framing simultaneously and both stress and rest phases at one-day protocol was performed for 50 patients. Data have been reconstructed by filter back projection (FBP) method and left ventricular (LV) systolic parameters were calculated by using QGS software. The effect of some factors such as LV cavity volume, presence and absence of perfusion defect, gender and type of study on data difference between 8 and 16 frames were evaluated. Results: The differences in ejection fraction (EF), end-diastolic volume (EDV) and end-systolic volume (ESV) in both stress and rest were statistically significant. Difference in both framing was more in stress for EF and ESV, and was more in rest for EDV. Study type had a significant effect on differences in systolic parameters while gender had a significant effect on differences in EF and ESV in rest between both framings. Conclusion: In conclusion, results of this study revealed that difference of both 16 and 8 frames data in systolic phase were statistically significant and it seems that because of better efficiency of 16 frames, it cannot be replaced by 8 frames. Further well-designed studies are required to verify these finding