1 research outputs found
Multifunctional Collagen and Hyaluronic Acid Multilayer Films on Live Mesenchymal Stem Cells
Cell
encapsulation has been reported to convey cytoprotective effects and
to better maintain cell survival. In contrast to other studies, our
report shows that the deposition of two major biomacromolecules, collagen
type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs)
does not entirely block the cell plasma membrane surface. Instead,
a considerable amount of the surface remained uncovered or only slightly
covered, as confirmed by TEM observation and by FACS analysis based
on quantitative surface labeling. Despite this structure showing openness
and flexibility, the multilayer Col/HA films significantly increased
cell survival in the attachment-deprived culture condition. In terms
of stem cell characteristics, the MSCs still showed functional cell
activity after film deposition, as evidenced by their colony-forming
activity and in vitro osteogenic differentiation. The Col/HA multilayer
films could provide a cytoprotective effect and induce osteogenic
differentiation without deteriorating effect or inhibition of cellular
attachment, showing that this technique can be a valuable tool for
modulating stem cell activities