Transgenesis and Genome Editing in Poultry

The transgenic approach and precise editing of specific loci in the genome have diverse practical uses in animal biotechnology. Recent advances in genome-editing technology, including clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) have helped to generate highly valuable and quality-improved poultry. The production of transgenic and genome-edited birds mainly depends on primordial germ cells (PGCs), which are the progenitor cells of gametes, due to the unique system that is quite different from the mammalian system. This chapter introduces the basic physiology of avian PGCs and the latest PGC-mediated methodologies in transgenesis and genome editing of birds. Based on these techniques, future applications of precisely genome-modulated poultry are discussed to provide opportunities and benefits for humans

The impact of IT vendor to firm’s IT outsourcing

This study seeks to better understand the impact of IT vendor to firm’s IT outsourcing in Korea. Using the event study methodology, we empirically analyze the impact of IT vendor in Korea. IT vendors’ characteristics (such as services with asset specificity, size, and nationality) that may affect the success of IT outsourcing are considered. We find that IT outsourcing announcements significantly increase firms’ market value in Korea. In terms of IT vendors’ characteristics, our study find that IT outsourcing announcements with large vendors are statistically significant while with small vendors are not. We also find that the market response to Korean IT vendor is significantly greater than to foreign vendor. However, unlike the US, the difference between IT outsourcing for high asset-specific service and for low asset-specific service is not found

Charge density functional plus UU calculation of lacunar spinel GaM4_4Se8_8 (M = Nb, Mo, Ta, and W)

Charge density functional plus $U$ calculations are carried out to examine the validity of molecular $J_\text{eff}$=1/2 and 3/2 state in lacunar spinel GaM$_4$X$_8$ (M = Nb, Mo, Ta, and W). With LDA (spin-unpolarized local density approximation)$+U$, which has recently been suggested as the more desirable choice than LSDA (local spin density approximation)$+U$, we examine the band structure in comparison with the previous prediction based on the spin-polarized version of functional and with the prototypical $J_\text{eff}$=1/2 material Sr$_2$IrO$_4$. It is found that the previously suggested $J_\text{eff}$=1/2 and 3/2 band characters remain valid still in LDA$+U$ calculations while the use of charge-only density causes some minor differences. Our result provides the further support for the novel molecular $J_\text{eff}$ state in this series of materials, which can hopefully motivate the future exploration toward its verification and the further search for new functionalities

Analysis of Laser ARPES from Bi2_2Sr2_2CaCu2_2O8+δ_{8+\delta} in superconductive state: angle resolved self-energy and fluctuation spectrum

We analyze the ultra high resolution laser angle resolved photo-emission spectroscopy (ARPES) intensity from the slightly underdoped Bi$_2$Sr$_2$CaCu$_2$O$_{8+\delta}$ in the superconductive (SC) state. The momentum distribution curves (MDC) were fitted at each energy \w employing the SC Green's function along several cuts perpendicular to the Fermi surface with the tilt angle $\theta$ with respect to the nodal cut. The clear observation of particle-hole mixing was utilized such that the complex self-energy as a function of $\omega$ is directly obtained from the fitting. The obtained angle resolved self-energy is then used to deduce the Eliashberg function \alpha^2 F^{(+)}(\th,\w) in the diagonal channel by inverting the d-wave Eliashberg equation using the maximum entropy method. Besides a broad featureless spectrum up to the cutoff energy $\omega_c$, the deduced $\alpha^2 F$ exhibits two peaks around 0.05 eV and 0.015 eV. The former and the broad feature are already present in the normal state, while the latter emerges only below $T_c$. Both peaks become enhanced as $T$ is lowered or the angle $\th$ moves away from the nodal direction. The implication of these findings are discussed.Comment: 7 pages, 5 figures, summited to PR

Energy-absorbing origami structure for crashworthiness design

This paper presents experimental and numerical investigations on the origami-patterned tube which is acknowledged as a promising energy-absorption device. Its buckling mode leads to high performances in terms of specific energy absorption (SEA) and crush force efficiency (CFE). The polygonal tube is prefolded by following an origami pattern, which is designed to act as geometric imperfection and mode inducer. First, a series of quasi-static crushing tests are performed on origami tubes with different materials and geometrical features. Specimens in SUS316L and AlSi10Mg are produced through Additive Manufacturing (AM). It allows to conveniently produce few samples with a complex shape. Finite Element Analysis (FEA) and Direct Image Correlation (DIC) are employed for a better insight into the complex crushing behaviour. The Aluminum tube shows a brittle behaviour while SUS316L tubes have extremely promising performance until local crack happens. Limits stemming from the employment of AM are explored and a new geometry is designed to avoid cracking. Second, a numerical design exploration study is carried out to assess the sensitivity of origami pattern features over the energy-absorption performance. ANSYS Autodyn is utilized as FE solver and DesignXplorer for correlation and optimization. The benefits of new patterns are investigated through geometrical optimization, and an improved geometry is proposed. The pattern stiffness is tuned to account for the external boundary conditions, resulting in a more uniform crushing behaviour. A similar force trend is maintained with a SEA increment of 51.7% due to a drastic weight reduction in areas with lower influence on post-buckling stiffnes

Precursor Phenomena of Barium Titanate Single Crystals Grown Using a Solid-State Single Crystal Growth Method Studied with Inelastic Brillouin Light Scattering and Birefringence Measurements

The nature of precursor phenomena in the paraelectric phase of ferroelectrics is one of the main questions to be resolved from a fundamental point of view. Barium titanate (BaTiO3) is one of the most representative perovskite-structured ferroelectrics intensively studied until now. The pretransitional behavior of BaTiO3 single crystal grown using a solid-state crystal growth (SSCG) method was investigated for the first time and compared to previous results. There is no melting process in the SSCG method, thus the crystal grown using a SSCG method have inherent higher levels of impurity and defect concentrations, which is a good candidate for investigating the effect of crystal quality on the precursor phenomena. The acoustic, dielectric, and piezoelectric properties, as well as birefringence, of the SSCG-grown BaTiO3 were examined over a wide temperature range. Especially, the acoustic phonon behavior was investigated in terms of Brillouin spectroscopy, which is a complementary technique to Raman spectroscopy. The obtained precursor anomalies of the SSCG-grown BaTiO3 in the cubic phase were similar to those of other single crystals, in particular, of high-quality single crystal grown by top-seeded solution growth method. These results clearly indicate that the observed precursor phenomena are common and intrinsic effect irrespective of the crystal quality