Raccoon Scavenging and the Taphonomic Effects on Early Human Decomposition and PMI Estimation

Forensic investigators must consider the effects of numerous taphonomic variables when estimating the postmortem interval (PMI) in forensic cases, such as temperature and insect activity. Efforts to quantify the decomposition process have been made. Megyesi et al. (2005) modified the categories and stages of decomposition presented by Galloway et al. (1989), and introduced a total body scoring system that uses accumulated degree-days to estimate PMI. However, none of these systems include the potential effects of animal scavenging. Galloway et al. (1989) stated that carnivorous activity occurs mainly during advanced decomposition and mummification. Haglund et al. (1989) specifically investigated canid scavenging and disarticulation patterns on human remains. Reeves (2009) studied the impact vultures have on human remains and how that information can be useful to forensic investigations. Synstelien (2015) and Jeong et al. (in press) document raccoon scavenging and how their seasonal activity impacts soft tissue decomposition. All of these studies demonstrate that animal activity on human remains may have a significant impact on current PMI estimation methods. This research investigates raccoon (Procyon lotor) scavenging during the early decomposition stage using daily photographs of ten individuals at University of Tennessee’s Anthropological Research Facility. All of the individuals were scavenged by raccoons. The onset and duration of scavenging was recorded, and then all total body scores and accumulated degree-days (ADD) were calculated using the methods set by Megyesi et al. (2005) to test for accuracy when including this variable in PMI estimation. The results of this research document how raccoon scavenging during fresh and early decomposition impacts estimation of PMI when applying Megyesi et al. (2005) total body scores and ADD calculations by causing accelerated skeletonization and mummification. It also stresses the importance of understanding the numerous variables that can influence the decomposition process (e.g. the relationship between scavenging and increased insect activity) that are not incorporated into current ADD calculations. This research demonstrates the need for understanding the numerous variables, including species-specific modifications, when estimating PMI in a forensic setting

Exploring Referee Abuse Through the Lens of the Collegiate Rugby Coach

Referees are valuable contributors to the legitimacy of a sporting contest. Despite this, abuse in sport has become a growing concern and is regularly noted as an obstacle with which referees must contend. Continued abuses have been associated with referee discontinuation and have been noted as a detrimental influence in the recruitment and retention of referees. Sporting organizations, coaches, and players all feel the impact when there is an inadequate supply of referees. Coaches have been noted as a primary perpetrator of referee abuse; thus, in this study, the authors utilize a phenomenological approach to explore perceptions of referee abuse through the lens of the collegiate rugby coach. Data were collected from 15 participants, all of whom completed two phone interviews. Five factors that influence one\u27s perceptions of and proclivity towards referee abuse emerged from the data: (a) personal characteristics/philosophies, (b) relationships, (c) social influences, (d) organizational expectations, and (e) culture. Implications to practice and recommendations for future research are discussed

Educational needs in families of pediatric liver and kidney transplant recipients: A quality improvement project

Parents of pediatric liver and kidney transplant recipients were surveyed regarding their current education plans (eg, Individualized Education Program, 504), satisfaction with these plans, and interest in educational support from the psychosocial transplant team. Survey results indicate high rates of IEP and 504 plans, academic and related services, and accommodations among this population. The majority of parents/guardians reported satisfaction with their child’s current school plan and did not report need for additional transplant team support specific to school services on the survey measure. However, other information highlights the importance for pediatric transplant teams to consider other ways to support this population’s educational needs.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149505/1/petr13412_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/149505/2/petr13412.pd

Lubricating Bacteria Model for Branching growth of Bacterial Colonies

Various bacterial strains (e.g. strains belonging to the genera Bacillus, Paenibacillus, Serratia and Salmonella) exhibit colonial branching patterns during growth on poor semi-solid substrates. These patterns reflect the bacterial cooperative self-organization. Central part of the cooperation is the collective formation of lubricant on top of the agar which enables the bacteria to swim. Hence it provides the colony means to advance towards the food. One method of modeling the colonial development is via coupled reaction-diffusion equations which describe the time evolution of the bacterial density and the concentrations of the relevant chemical fields. This idea has been pursued by a number of groups. Here we present an additional model which specifically includes an evolution equation for the lubricant excreted by the bacteria. We show that when the diffusion of the fluid is governed by nonlinear diffusion coefficient branching patterns evolves. We study the effect of the rates of emission and decomposition of the lubricant fluid on the observed patterns. The results are compared with experimental observations. We also include fields of chemotactic agents and food chemotaxis and conclude that these features are needed in order to explain the observations.Comment: 1 latex file, 16 jpeg files, submitted to Phys. Rev.

Probing the potential of mucus permeability to signify preterm birth risk

Preterm birth is the leading cause of neonatal mortality, and is frequently associated with intra-amniotic infection hypothesized to arise from bacterial ascension across a dysfunctional cervical mucus plug. To study this dysfunction, we assessed the permeability of cervical mucus from non-pregnant ovulating (n = 20) and high-(n = 9) and low-risk (n = 16) pregnant women to probes of varying sizes and surface chemistries. We found that the motion of negatively charged, carboxylated microspheres in mucus from pregnant patients was significantly restricted compared to ovulating patients, but not significantly different between high-A nd low-risk pregnant women. In contrast, charged peptide probes small enough to avoid steric interactions, but sensitive to the biochemical modifications of mucus components exhibited significantly different transport profiles through mucus from high-A nd low-risk patients. Thus, although both microstructural rearrangements of the components of mucus as well as biochemical modifications to their adhesiveness may alter the overall permeability of the cervical mucus plug, our findings suggest that the latter mechanism plays a dominant role in the impairment of the function of this barrier during preterm birth. We expect that these probes may be readily adapted to study the mechanisms underlying disease progression on all mucosal epithelia, including those in the mouth, lungs, and gut.National Science Foundation (U.S.) (Award DMR-0819762)National Science Foundation (U.S.) (Award 1122374

Global distributed evolution of L-systems fractals

Internet based parallel genetic programming (GP) creates fractal patterns like Koch’s snow flake. Pfeiffer, http://www.cs.ucl.ac.uk/staff/W.Langdon/pfeiffer.html, by analogy with seed/embryo development, uses Lindenmayer grammars and LOGO style turtle graphics written in Javascript and Perl. 298 novel pictures were produced. Images are placed in animated snow globes (computerised snowstorms) by www web browsers anywhere on the planet. We discuss artificial life (Alife) evolving autonomous agents and virtual creatures in higher dimensions from a free format representation in the context of neutral networks, gene duplication and the evolution of higher order genetic operators

Management of Intracranial Meningiomas Using Keyhole Techniques

BACKGROUND: Keyhole craniotomies are increasingly being used for lesions of the skull base. Here we review our recent experience with these approaches for resection of intracranial meningiomas. METHODS: Clinical and operative data were gathered on all patients treated with keyhole approaches by the senior author from January 2012 to June 2013. Thirty-one meningiomas were resected in 27 patients, including 9 supratentorial, 5 anterior fossa, 7 middle fossa, 6 posterior fossa, and 4 complex skull base tumors. Twenty-nine tumors were WHO Grade I, and 2 were Grade II. RESULTS: The mean operative time was 8 hours, 22 minutes (range, 2:55-16:14) for skull-base tumors, and 4 hours, 27 minutes (range, 1:45-7:13) for supratentorial tumors. Simpson Resection grades were as follows: Grade I = 8, II = 8, III = 1, IV = 15, V = 0. The median postoperative hospital stay was 4 days (range, 1-20 days). In the 9 patients presenting with some degree of visual loss, 7 saw improvement or complete resolution. In the 6 patients presenting with cranial nerve palsies, 4 experienced improvement or resolution of the deficit postoperatively. Four patients experienced new neurologic deficits, all of which were improved or resolved at the time of the last follow-up. Technical aspects and surgical nuances of these approaches for management of intracranial meningiomas are discussed. CONCLUSIONS: With careful preoperative evaluation, keyhole approaches can be utilized singly or in combination to manage meningiomas in a wide variety of locations with satisfactory results

Optimality of mutation and selection in germinal centers

The population dynamics theory of B cells in a typical germinal center could play an important role in revealing how affinity maturation is achieved. However, the existing models encountered some conflicts with experiments. To resolve these conflicts, we present a coarse-grained model to calculate the B cell population development in affinity maturation, which allows a comprehensive analysis of its parameter space to look for optimal values of mutation rate, selection strength, and initial antibody-antigen binding level that maximize the affinity improvement. With these optimized parameters, the model is compatible with the experimental observations such as the ~100-fold affinity improvements, the number of mutations, the hypermutation rate, and the "all or none" phenomenon. Moreover, we study the reasons behind the optimal parameters. The optimal mutation rate, in agreement with the hypermutation rate in vivo, results from a tradeoff between accumulating enough beneficial mutations and avoiding too many deleterious or lethal mutations. The optimal selection strength evolves as a balance between the need for affinity improvement and the requirement to pass the population bottleneck. These findings point to the conclusion that germinal centers have been optimized by evolution to generate strong affinity antibodies effectively and rapidly. In addition, we study the enhancement of affinity improvement due to B cell migration between germinal centers. These results could enhance our understandings to the functions of germinal centers.Comment: 5 figures in main text, and 4 figures in Supplementary Informatio