Bond strength of Resilon/Epiphany compared with Gutta-percha and sealers Sealer 26 and Endo Fill

This study evaluated the bond strength of Epiphany (R)/Resilon (R) to radicular dentine and compared this with that of Gutta-percha sealed with Sealer 26 and also Endo Fill (R). Fifteen human single-rooted extracted teeth had their root canals enlarged to a standardised internal root diameter of 1.5 mm. The teeth were filled with: Group I Epiphany (R)/Resilon (R) cones, Group II Sealer 26 (R)/Gutta-percha cones and Group III Endo Fill (R)/Gutta-percha cones. Three 2 mm-thick cross-sections were obtained from each tooth (n = 15) and subjected to a push-out test (1 mm min-1). The means (MPa) were compared using anova /NewmanKeuls tests. There were no statistically significant differences between Group I and Group II (P > 0.01). Both bond strengths were significantly higher than that of Group III (P < 0.01). The root canal filling system Epiphany (R)/Resilon (R) showed bond strength similar to that of Sealer 26 (R)/Gutta-percha. Both were higher than that of Endo Fill (R)/Gutta-percha.381212

Screening of Aspergillus strains isolated from vineyards for pectinase production

Pectin is present in walls of plant cells. Pectinases are important for food and chemical industry that processes plant material, and also juice, textile and vine industry uses pectinases. They are able to modify and cause depolymerization in pectin chains. Fungi from genus Aspergillus are one of the most important sources of these enzymes. Vineyards contain plant material in decomposition and are a special place for pectinase producers. Many works screening pectinase producers were described in literature. However, better producers have been continuously found. This paper describes a screening of Aspergillus strains isolated from vineyards for pectinase production. The fungi strains (262) were evaluated in the screening methodology that studied the diameter of the pectin hydrolysis halo and the size of the colony in an agar culture media containing pectin as the sole carbon source. Four strains were selected for fermentation experiments. Aspergillus foetidus var. pallidus Ege-K-730 and Aspergillus aculeatus Ege-K-355 presented the highest pectinase production ratio, and Aspergillus foetidus var. pallidus K-635 and Aspergillus aculeatus K-398 presented the highest colony diameter. Aspergillus carbonarius CFTRI 1047 was used as a reference strain. In submerged fermentation (SbF), the strains Ege-K-730, Ege-K-355, Ege-K-635 and CFTRI 1047 produced the highest PG (polygalacturonase) and PMG (polymethylgalacturonase) levels, however, Ege-K-730 was the faster producer. In solid state fermentation (SSF), the strains Ege-K-730, Ege-K-635, Ege-K-398 and CFTRI 1047 produced the highest PG and PMG levels. In the experimental conditions, SSF was better than SbF for pectinase production

Evaluation of the colour change in enamel and dentine promoted by the interaction between 2% chlorhexidine and auxiliary chemical solutions

To evaluate the colour change in enamel and dentine, promoted by interaction of 2% chlorhexidine gluconate (CHX) with 5.25% sodium hypochlorite (NaOCl) and 17% ethylenediaminetetraacetic acid (EDTA). Fragments containing enamel and dentine were obtained from the crowns of extracted bovine incisors. Before and after immersion of the samples in the substances, they were evaluated with reference to the colour of the enamel and dentine. The values obtained in numerical scores were subjected to statistical analysis using Wilcoxon test. A colour change in the enamel and dentine in groups treated with CHX gel+NaOCl and CHX gel+NaOCl+EDTA, and a change in colour only in the dentine in groups treated with CHX solution+NaOCl and CHX solution+NaOCl+EDTA. When used prior to NaOCl, CHX has the ability to induce a colour change in dental structures.39310711