Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7

716749Made available in DSpace on 2019-09-11T20:51:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2015This study aimed to evaluate the capacity of Lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (TNF-α, IL-1β, IL-4, IL-6, IL-10, and IL-12) by mouse macrophages (RAW 264.7). Three microorganism preparations were used: live L. rhamnosus (LLR) suspension, heat-killed L. rhamnosus (HKLR) suspension, and the supernatant of a heat-killed L. rhamnosus (SHKLR) suspension, which were cultured with macrophages (37°C, 5% CO2) for 2 h and 30 min. After that, cells were cultured for 16 h. The supernatants were used for the quantitation of cytokines, by ELISA. The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%. LLR and HKLR groups were able to significantly increase the production of TNF-α, IL-6, and IL-10 (P < 0. 05). SHKLR also significantly increased the production of TNF-α and IL-10 (P < 0. 05) but not IL-6 (P > 0. 05). All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0. 05). In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses. © 2015 Adeline Lacerda Jorjão et al.Jorjão, A.L., Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, BrazilDe Oliveira, F.E., Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, BrazilLeão, M.V.P., Bioscience Basic Institute, University of Taubaté, Street Expedicionário Ernesto Pereira 3, Taubaté, SP, 12020-130, BrazilCarvalho, C.A.T., Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, BrazilJorge, A.O.C., Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, BrazilDe Oliveira, L.D., Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, Brazi

The next generation of laser spectroscopy experiments using light muonic atoms

Precision spectroscopy of light muonic atoms provides unique information about the atomic and nuclear structure of these systems and thus represents a way to access fundamental interactions, properties and constants. One application comprises the determination of absolute nuclear charge radii with unprecedented accuracy from measurements of the 2S - 2P Lamb shift. Here, we review recent results of nuclear charge radii extracted from muonic hydrogen and helium spectroscopy and present experiment proposals to access light muonic atoms with Z ≥ 3. In addition, our approaches towards a precise measurement of the Zemach radii in muonic hydrogen (μp) and helium (μ 3He+) are discussed. These results will provide new tests of bound-state quantum-electrodynamics in hydrogen-like systems and can be used as benchmarks for nuclear structure theories.ISSN:1742-6588ISSN:1742-659