32 research outputs found
Glucagon-like peptide-1(7–36) amide is a new incretin/enterogastrone candidate
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72032/1/j.1365-2362.1991.tb01802.x.pd
Immunocytochemical evidence for the presence of gamma 1-MSH-like immunoreactivity in pituitary corticotrophs and ACTH-producing tumours.
The presence of gamma 1-MSH has been demonstrated in bovine neuro-intermediate
lobe by biochemical methods, thus suggesting that this peptide is cleaved from
the cryptic region of pro-opiocortin. In this study we report the localisation of
gamma 1-MSH-like immunoreactivity in the adenohypophysis of man, ox, pig, dog and
guinea-pig using immunocytochemical procedures at both light and electron
microscope levels. Antisera recognising the C-terminal Arg-Phe-amide and the
C-terminal penta-peptide-amide of gamma 1-MSH have been used throughout this
study. The immunostaining was found in all endocrine cells of the pars intermedia
(where present) and in scattered cells of the pars distalis identified as
corticotrophs. No gamma 1-MSH immunoreactivity was detected in rat
adenohypophysis. In addition, 7 ACTH-producing tumours (1 pituitary adenoma and 6
ectopic) were investigated and shown to contain gamma 1-MSH immunoreactive cells