INSIGHT INTO ANTIMICROBIC EVALUATION TECHNIQUES AND THEIR ROLE IN BETTER ASSESSMENT OF ANTIMICROBIC AGENTS: A REVIEW

At present, researchers have a keen interest in creating advanced antimicrobic agents to overcome microbial resistance from various new sources either from plants or microbes. For this, robust attention has been given to the advancement in the rapid antimicrobic assessment strategies and methods to develop antimicrobic agents. Some of the antimicrobic techniques such as well diffusion using broth and agar and disc diffusion are more prominent in assaying antimicrobial testing while techniques such as bioluminescence, flow cytofluorometry methods are less used due to specific equipment’s, high calibration and evaluation processes. Thus, the information of precise antimicrobial techniques is must to the new researchers for antimicrobic testing. In this review article, various antimicrobic techniques with their advantages and limitations are being reported which are currently being carried out for antimicrobial testing

IMMOBILIZATION OF AMYLASE PRODUCING BACTERIA (BACILLUS SUBTILIS) FROM SOIL OF WESTERN HIMALAYAN REGION SOLAN, HIMACHAL PRADESH, INDIA

Objective: The paper aimed to immobilize amylase producing bacterial strain on a suitable matrix and characterization of its physicochemical properties so that much amount of amylase could be produced to be applied in different industries. Methods: Bacterial colonies were sub-cultured from samples collected from soil in freshly prepared dishes containing starch agar by dot method using sterile inoculating needles from which five different bacteria belonged to genus Bacillus were isolated and assigned as A1, A2, A3, A4, and A5. Results: It was found that A1 displayed the highest enzyme activity of 17.89 IU/ml with enzyme assay of 0.83 mg/ml and the bacterium was identified to be Bacillus subtilis. A5 displayed 10.13 IU/ml with protein contents of 0.11 mg/ml indicated that A1 possess the highest enzyme activities which were categorized under Bacillus and protein contents and A5 showed less amount of enzyme activities and protein contents as compared to other. Conclusion: The bacteria which were produced much amount of enzyme activities identified as Bacillus subtilis and recommended and have been recommended to be cultured for the production of amylase enzyme

Earthworms (Annelida: Clitellata: Megadrili) of Solan, a constituent of Himalayan Biodiversity Hotspot, India

The present work is an update on the earthworm fauna of Solan District, Himachal Pradesh State, a constituent of Himalayan biodiversity hotspot. It is based on the field collection and published litera- ture data. Hereby, it is provided an updated list of 32 species belonging to 18 genera and seven families, namely Moniligastridae, Lumbricidae, Ocnerodrilidae, Acanthodrilidae, Benhamiidae, Octochaetidae and Megascolecidae. These mainly include exotic and native peregrine species, including exotic per- egrine Amynthas hupeiensis (Michaelsen, 1895), which was recently recorded for the first time from India. A systematic account of earthworm species with their distribution and a dichotomous key is provided for their identification

New species and new records of earthworms of the genus Drawida from Kerala part of the Western Ghats biodiversity hotspot, India (Oligochaeta, Moniligastridae)

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A comprehensive checklist of the earthworms (Annelida: Clitellata: Megadrili) of Sri Lanka, a component of the Western Ghats – Sri Lanka biodiversity hotspot

A comprehensive checklist of the earthworm species known from Sri Lanka, an island country in the Indian subcontinent, is presented. In total, 81 species and subspecies belonging to 20 genera and 8 families are recorded. Of these, 58 are Sri Lanka endemics, 2 near endemics and 21 exotics. The checklist includes the type locality, any significant subsequent generic placements, and the distributional pattern with province and districts for each species/subspecies within Sri Lanka

Osteosarcoma as a Second Malignant Disease in a Case of Bilateral Retinoblastoma

Patients with hereditary retinoblastoma, whether treated by radiation or not, are at an increased risk of subsequently developing sarcomas as a second malignancy. As the understanding of the underlying genetics of osteosarcoma and retinoblastoma has improved, a clear association between the two entities has been noted. We present a case of a young patient with previous history of bilateral retinoblastoma who was treated by surgery and chemoradiation for his primary disease and subsequent recurrences. Thirteen years after his initial diagnosis, he developed osteosarcoma of the mandible as the second primary lesion and was treated with surgery and chemotherapy. The intent of the case report is to bring forth the current thinking of genetic association between retinoblastoma and osteosarcoma and to discuss aggressive treatment protocol for the latter, in the form of surgery and chemotherapy