4 research outputs found
Soybean Toxin (SBTX) Impairs Fungal Growth by Interfering with Molecular Transport, Carbohydrate/Amino Acid Metabolism and Drug/Stress Responses
<p>Soybean toxin (SBTX) is an antifungal protein from soybeans with broad inhibitory activity against the growth and filamentation of many fungi, including human and plant pathogenic species such as Candida albicans, Candida parapsilosis, Aspergillus niger, Penicillium herquei, Cercospora sojina and Cercospora kikuchii. Understanding the mechanism by which SBTX acts on fungi and yeasts may contribute to the design of novel antifungal drugs and/or the development of transgenic plants resistant to pathogens. To this end, the polymorphic yeast C. albicans was chosen as a model organism and changes in the gene expression profile of strain SC5314 upon exposure to SBTX were examined. Genes that were differentially regulated in the presence of SBTX were involved in glucose transport and starvation-associated stress responses as well as in the control of both the induction and repression of C. albicans hyphal formation. Transmission electron microscopy showed that C. albicans cells exposed to SBTX displayed severe signs of starvation and were heavily granulated. Our data were indicative of C. albicans cell starvation despite sufficient nutrient availability in the medium; therefore, it can be speculated that SBTX blocks nutrient uptake systems. Because neither the starvation signal nor the alkaline response pathway lead to the induction of hyphae, we hypothesise that conflicting signals are transmitted to the complex regulatory network controlling morphogenesis, eventually preventing the filamentation signal from reaching a significant threshold.</p>
New Insights into the Structure and Mode of Action of Mo-CBP3, an Antifungal Chitin-Binding Protein of Moringa oleifera Seeds
Natural compounds extracted from Moringa oleifera and their agricultural applications
Natural bio-active compounds synthesized by plants as secondary metabolites are well known and established. Today, their application in various fields such as medicine in the form of drugs and biopesticides in agriculture is well documented. In recent times, the delivery of such compounds is achieved through nanodelivery technology, which is gaining acceptability in both field of drugs and agrochemical industries. The bio-active compounds with chemical diversity are obtained from nature either as homogenous plant crude extracts or as purified compounds. Crude plant extracts exist as a combination of different bio-active compounds with various polarities, and their partition remains a challenge in the process of characterization and identification. Extraction of these compounds from plant species is achieved by different solvents and extraction methods. Analytical methods like HPLC have commonly been utilized with GC-MS and LC-MS/MS chromatography methods to identify the compounds. Crude extracts from different morphological parts of plant species including Moringa oleifera are increasingly becoming important in the context of agricultural pest management and human medicine. M. oleifera is a medicinal plant that synthesizes such metabolites which include phenolic acids, carotenoids, quinones, antraquinones, flavonoids, flavonols, flavones, tannins, alkaloids, coumarins, terpenoids, amines, cyanogenic glycosides, triterpenoids, non-protein amino acids, glucosinolates, polyacetylenes, polyketides, phenylpropanes, steroids and saponins. They exert biological activities and can potentially be used to retard microbial activities. Other uses of M. oleifera are medicinal uses and other purposes such as water purification, fertilizer, biogas and biopesticides. The aim of this chapter is to highlight the uses and profiling of bio-active compounds of M. oleifera, their mode of action and prospects in commercial biopesticides for agricultural applications