Comparative Analysis of the 5S rRNA and Its Associated Proteins Reveals Unique Primitive Rather Than Parasitic Features in Giardia lamblia

BACKGROUND: 5S rRNA is a highly conserved ribosomal component. Eukaryotic 5S rRNA and its associated proteins (5S rRNA system) have become very well understood. Giardia lamblia was thought by some researchers to be the most primitive extant eukaryote while others considered it a highly evolved parasite. Previous reports have indicated that some aspects of its 5S rRNA system are simpler than that of common eukaryotes. We here explore whether this is true to its entire system, and whether this simplicity is a primitive or parasitic feature. METHODOLOGY/PRINCIPAL FINDINGS: By collecting and confirming pre-existing data and identifying new data, we obtained almost complete datasets of the system of three isolates of G. lamblia, two other parasitic excavates (Trichomonas vaginalis, Trypanosoma cruzi), and one free-living one (Naegleria gruberi). After comprehensively comparing each aspect of the system among these excavates and also with those of archaea and common eukaryotes, we found all the three Giardia isolates to harbor a same simplified 5S rRNA system, which is not only much simpler than that of common eukaryotes but also the simplest one among those of these excavates, and is surprisingly very similar to that of archaea; we also found among these excavates the system in parasitic species is not necessarily simpler than that in free-living species, conversely, the system of free-living species is even simpler in some respects than those of parasitic ones. CONCLUSION/SIGNIFICANCE: The simplicity of Giardia 5S rRNA system should be considered a primitive rather than parasitically-degenerated feature. Therefore, Giardia 5S rRNA system might be a primitive system that is intermediate between that of archaea and the common eukaryotic model system, and it may reflect the evolutionary history of the eukaryotic 5S rRNA system from the archaeal form. Our results also imply G. lamblia might be a primitive eukaryote with secondary parasitically-degenerated features

Partner in fat metabolism: role of KLFs in fat burning and reproductive behavior

The abnormalities caused by excess fat accumulation can result in pathological conditions which are linked to several interrelated diseases, such as cardiovascular disease and obesity. This set of conditions, known as metabolic syndrome, is a global pandemic of enormous medical, economic, and social concern affecting a significant portion of the world’s population. Although genetics, physiology and environmental components play a major role in the onset of disease caused by excessive fat accumulation, little is known about how or to what extent each of these factors contributes to it. The worm, Caenorhabditis elegans offers an opportunity to study disease related to metabolic disorder in a developmental system that provides anatomical and genomic simplicity relative to the vertebrate animals and is an excellent eukaryotic genetic model which enable us to answer the questions concerning fat accumulation which remain unresolved. The stored triglycerides (TG) provide the primary source of energy during periods of food deficiency. In nature, lipid stored as TGs are hydrolyzed into fatty acids which are broken down through β-oxidation to yield acetyl-CoA. Our recent study suggests that a member of C. elegans Krüppel-like factor, klf-3 regulates lipid metabolism by promoting FA β-oxidation and in parallel may contribute in normal reproduction and fecundity. Genetic and epigenetic factors that influence this pathway may have considerable impact on fat related diseases in human. Increasing number of studies suggest the role of mammalian KLFs in adipogenesis. This functional conservation should guide our further effort to explore C. elegans as a legitimate model system for studying the role of KLFs in many pathway components of lipid metabolism

A New Mechanistic Scenario for the Origin and Evolution of Vertebrate Cartilage

The appearance of cellular cartilage was a defining event in vertebrate evolution because it made possible the physical expansion of the vertebrate “new head”. Despite its central role in vertebrate evolution, the origin of cellular cartilage has been difficult to understand. This is largely due to a lack of informative evolutionary intermediates linking vertebrate cellular cartilage to the acellular cartilage of invertebrate chordates. The basal jawless vertebrate, lamprey, has long been considered key to understanding the evolution of vertebrate cartilage. However, histological analyses of the lamprey head skeleton suggest it is composed of modern cellular cartilage and a putatively unrelated connective tissue called mucocartilage, with no obvious transitional tissue. Here we take a molecular approach to better understand the evolutionary relationships between lamprey cellular cartilage, gnathostome cellular cartilage, and lamprey mucocartilage. We find that despite overt histological similarity, lamprey and gnathostome cellular cartilage utilize divergent gene regulatory networks (GRNs). While the gnathostome cellular cartilage GRN broadly incorporates Runx, Barx, and Alx transcription factors, lamprey cellular cartilage does not express Runx or Barx, and only deploys Alx genes in certain regions. Furthermore, we find that lamprey mucocartilage, despite its distinctive mesenchymal morphology, deploys every component of the gnathostome cartilage GRN, albeit in different domains. Based on these findings, and previous work, we propose a stepwise model for the evolution of vertebrate cellular cartilage in which the appearance of a generic neural crest-derived skeletal tissue was followed by a phase of skeletal tissue diversification in early agnathans. In the gnathostome lineage, a single type of rigid cellular cartilage became dominant, replacing other skeletal tissues and evolving via gene cooption to become the definitive cellular cartilage of modern jawed vertebrates

Measurement of CP observables in the process B ⁰ → DK ^*0 with two- and four-body D decays

Measurements of $C\!P$ observables in $B^0 \to DK^{*0}$ decays are presented, where $D$ represents a superposition of $D^0$ and $\bar{D}^0$ states. The $D$ meson is reconstructed in the two-body final states $K^+\pi^-$, $\pi^+ K^-$, $K^+K^-$ and $\pi^+\pi^-$, and, for the first time, in the four-body final states $K^+\pi^-\pi^+\pi^-$, $\pi^+ K^-\pi^+\pi^-$ and $\pi^+\pi^-\pi^+\pi^-$. The analysis uses a sample of neutral $B$ mesons produced in proton-proton collisions, corresponding to an integrated luminosity of 1.0, 2.0 and 1.8 $\rm fb^{-1}$ collected with the LHCb detector at centre-of-mass energies of $\sqrt{s} =$ 7, 8 and 13 TeV, respectively. First observations of the decays $B^0 \to D(\pi^+ K^-)K^{*0}$ and $B^0 \to D(\pi^+\pi^-\pi^+\pi^-)K^{*0}$ are obtained. The measured observables are interpreted in terms of the $C\!P$-violating weak phase $\gamma$

Updated measurement of time-dependent CP -violating observables in Bs0→J/ψ^K+^K- decays

The decay-time-dependent $CP$ asymmetry in $B^{0}_{s}\to J/\psi K^{+} K^{-}$ decays is measured using proton-proton collision data, corresponding to an integrated luminosity of $1.9\,\mathrm{fb^{-1}}$, collected with the LHCb detector at a centre-of-mass energy of $13\,\mathrm{TeV}$ in 2015 and 2016. Using a sample of approximately 117\,000 signal decays with an invariant $K^{+} K^{-}$ mass in the vicinity of the $\phi(1020)$ resonance, the $CP$-violating phase $\phi_s$ is measured, along with the difference in decay widths of the light and heavy mass eigenstates of the $B^{0}_{s}$-$\bar{B}^{0}_{s}$ system, $\Delta\Gamma_s$. The difference of the average $B^{0}_{s}$ and $B^{0}$ meson decay widths, $\Gamma_s-\Gamma_d$, is determined using in addition a sample of $B^{0} \to J/\psi K^{+} \pi^{-}$ decays. The values obtained are $\phi_s = -0.083\pm0.041\pm0.006\,\mathrm{rad}$, $\Delta\Gamma_s = 0.077 \pm 0.008 \pm 0.003\, \mathrm{ps^{-1}}$ and $\Gamma_s-\Gamma_d = -0.0041 \pm 0.0024 \pm 0.0015\,\mathrm{ps^{-1}}$, where the first uncertainty is statistical and the second systematic. These are the most precise single measurements of these quantities to date and are consistent with expectations based on the Standard Model and with a previous LHCb analysis of this decay using data recorded at centre-of-mass energies 7 and 8 TeV. Finally, the results are combined with recent results from $B^{0}_{s}\to J/\psi \pi^{+} \pi^{-}$ decays obtained using the same dataset as this analysis, and with previous independent LHCb results

Observation of the Λb0→χc1 (3872) pK⁻ decay

Using proton-proton collision data, collected with the LHCb detector and corresponding to 1.0, 2.0 and 1.9fb$^{-1}$ of integrated luminosity at the centre-of-mass energies of 7, 8, and 13 TeV, respectively, the decay $\Lambda_b^0\to \chi_{c1}(3872)pK^-$ with $\chi_{c1}\to J/\psi\pi^+\pi^-$ is observed for the first time. The significance of the observed signal is in excess of seven standard deviations. It is found that $(58\pm15)\%$ of the decays proceed via the two-body intermediate state $\chi_{c1}(3872)\Lambda(1520)$. The~branching fraction with respect to that of the $\Lambda_b\rightarrow\psi(2S)p K^{-}$ decay mode, where the $\psi(2S)$~meson is reconstructed in the $J/\psi \pi^+\pi^-$ final state, is measured to be: \begin{equation*} \frac{\Lambda_b^0\to\chi_{c1}(3872)pK^-}{\Lambda_b\to\psi(2S)p K^-} \times \frac{\mathcal{B}(\chi_{c1} \to J/\psi \pi^+\pi^-)}{\mathcal{B}(\psi(2S)\to J/\psi \pi^+\pi^-)} = \left(5.4 \pm 1.1 \pm 0.2\right)\times 10^{-2}\,, \end{equation*} where the first uncertainty is statistical and the second is systematic

SecA, a remarkable nanomachine

Biological cells harbor a variety of molecular machines that carry out mechanical work at the nanoscale. One of these nanomachines is the bacterial motor protein SecA which translocates secretory proteins through the protein-conducting membrane channel SecYEG. SecA converts chemically stored energy in the form of ATP into a mechanical force to drive polypeptide transport through SecYEG and across the cytoplasmic membrane. In order to accommodate a translocating polypeptide chain and to release transmembrane segments of membrane proteins into the lipid bilayer, SecYEG needs to open its central channel and the lateral gate. Recent crystal structures provide a detailed insight into the rearrangements required for channel opening. Here, we review our current understanding of the mode of operation of the SecA motor protein in concert with the dynamic SecYEG channel. We conclude with a new model for SecA-mediated protein translocation that unifies previous conflicting data

Test of lepton universality in b→sℓ+ℓ−b \rightarrow s \ell^+ \ell^- decays

The first simultaneous test of muon-electron universality using $B^{+}\rightarrow K^{+}\ell^{+}\ell^{-}$ and $B^{0}\rightarrow K^{*0}\ell^{+}\ell^{-}$ decays is performed, in two ranges of the dilepton invariant-mass squared, $q^{2}$. The analysis uses beauty mesons produced in proton-proton collisions collected with the LHCb detector between 2011 and 2018, corresponding to an integrated luminosity of 9 $\mathrm{fb}^{-1}$. Each of the four lepton universality measurements reported is either the first in the given $q^{2}$ interval or supersedes previous LHCb measurements. The results are compatible with the predictions of the Standard Model.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-046.html (LHCb public pages