19 research outputs found
PolyADP-Ribosylation Is Required for Pronuclear Fusion during Postfertilization in Mice
BACKGROUND: During fertilization, pronuclear envelope breakdown (PNEB) is followed by the mingling of male and female genomes. Dynamic chromatin and protein rearrangements require posttranslational modification (PTM) for the postfertilization development. METHODOLOGY/PRINCIPAL FINDINGS: Inhibition of poly(ADP-ribose) polymerase activity (PARylation) by either PJ-34 or 5-AIQ resulted in developmental arrest of fertilized embryos at the PNEB. PARylation inhibition affects spindle bundle formation and phosphorylation of Erk molecules of metaphase II (MII) unfertilized oocytes. We found a frequent appearance of multiple pronuclei (PN) in the PARylation-inhibited embryos, suggesting defective polymerization of tubulins. Attenuated phosphorylation of lamin A/C by PARylation was detected in the PARylation-inhibited embryos at PNEB. This was associated with sustained localization of heterodomain protein 1 (HP1) at the PN of the one-cell embryos arrested by PARylation inhibition. CONCLUSIONS/SIGNIFICANCE: Our findings indicate that PARylation is required for pronuclear fusion during postfertilization processes. These data further suggest that PARylation regulates protein dynamics essential for the beginning of mouse zygotic development. PARylation and its involving signal-pathways may represent potential targets as contraceptives
HIV-1 Populations in Semen Arise through Multiple Mechanisms
HIV-1 is present in anatomical compartments and bodily fluids. Most transmissions occur through sexual acts, making virus in semen the proximal source in male donors. We find three distinct relationships in comparing viral RNA populations between blood and semen in men with chronic HIV-1 infection, and we propose that the viral populations in semen arise by multiple mechanisms including: direct import of virus, oligoclonal amplification within the seminal tract, or compartmentalization. In addition, we find significant enrichment of six out of nineteen cytokines and chemokines in semen of both HIV-infected and uninfected men, and another seven further enriched in infected individuals. The enrichment of cytokines involved in innate immunity in the seminal tract, complemented with chemokines in infected men, creates an environment conducive to T cell activation and viral replication. These studies define different relationships between virus in blood and semen that can significantly alter the composition of the viral population at the source that is most proximal to the transmitted virus
The Detectability of Earth's Biosignatures Across Time
Over the past two decades, enormous advances in the detection of exoplanets
have taken place. Currently, we have discovered hundreds of earth-sized
planets, several of them within the habitable zone of their star. In the coming
years, the efforts will concentrate in the characterization of these planets
and their atmospheres to try to detect the presence of biosignatures. However,
even if we discovered a second Earth, it is very unlikely that it would present
a stage of evolution similar to the present-day Earth. Our planet has been far
from static since its formation about 4.5 Ga ago; on the contrary, during this
time, it has undergone multiple changes in it's atmospheric composition, it's
temperature structure, it's continental distribution, and even changes in the
forms of life that inhabit it. All these changes have affected the global
properties of Earth as seen from an astronomical distance. Thus, it is of
interest not only to characterize the observables of the Earth as it is today,
but also at different epochs. Here we review the detectability of the Earth's
globally-averaged properties over time. This includes atmospheric composition
and biosignatures, and surface properties that can be interpreted as sings of
habitability (bioclues). The resulting picture is that truly unambiguous
biosignatures are only detectable for about 1/4 of the Earth's history. The
rest of the time we rely on detectable bioclues that can only establish an
statistical likelihood for the presence of life on a given planet.Comment: To appear in "Handbook of Exoplanets", eds. Deeg, H.J. & Belmonte,
J.A, Springer (2018). arXiv admin note: text overlap with
arXiv:astro-ph/0609398 by other author
Earth: Atmospheric Evolution of a Habitable Planet
Our present-day atmosphere is often used as an analog for potentially
habitable exoplanets, but Earth's atmosphere has changed dramatically
throughout its 4.5 billion year history. For example, molecular oxygen is
abundant in the atmosphere today but was absent on the early Earth. Meanwhile,
the physical and chemical evolution of Earth's atmosphere has also resulted in
major swings in surface temperature, at times resulting in extreme glaciation
or warm greenhouse climates. Despite this dynamic and occasionally dramatic
history, the Earth has been persistently habitable--and, in fact,
inhabited--for roughly 4 billion years. Understanding Earth's momentous changes
and its enduring habitability is essential as a guide to the diversity of
habitable planetary environments that may exist beyond our solar system and for
ultimately recognizing spectroscopic fingerprints of life elsewhere in the
Universe. Here, we review long-term trends in the composition of Earth's
atmosphere as it relates to both planetary habitability and inhabitation. We
focus on gases that may serve as habitability markers (CO2, N2) or
biosignatures (CH4, O2), especially as related to the redox evolution of the
atmosphere and the coupled evolution of Earth's climate system. We emphasize
that in the search for Earth-like planets we must be mindful that the example
provided by the modern atmosphere merely represents a single snapshot of
Earth's long-term evolution. In exploring the many former states of our own
planet, we emphasize Earth's atmospheric evolution during the Archean,
Proterozoic, and Phanerozoic eons, but we conclude with a brief discussion of
potential atmospheric trajectories into the distant future, many millions to
billions of years from now. All of these 'Alternative Earth' scenarios provide
insight to the potential diversity of Earth-like, habitable, and inhabited
worlds.Comment: 34 pages, 4 figures, 4 tables. Review chapter to appear in Handbook
of Exoplanet
Estimating upper stem diameters and volume of Douglas-fir and Western hemlock trees in the Pacific northwest
Explicit cloud representation in the <i>Atmos</i> 1D climate model for Earth and rocky planet applications
Spatially explicit competition in a mixed planting of Araucaria cunninghamii and Flindersia brayleyana
• Context: A 20-year-old Nelder wheel planted with hoop pine (Araucaria cunninghamii Aiton ex D.Don) and Queensland maple (Flindersia brayleyana F.Muell.) in 18 spokes and 8 rings represents nominal point densities of 3,580, 2,150, 1,140, 595, 305, 158, 82, and 42 stems/ha and offers an opportunity to examine competition and spatial interaction between these two species. • Aims: This study aimed to evaluate the intraspecific and interspecific competition between two contrasting tree species and to determine the distance over which competition can be observed. • Methods: Competition was estimated using Hegyi's index, implemented using the Simile visual modeling environment, and calibrated using nonlinear least squares with PEST. • Results: Interactions were detected between pairs of stems closer than D < 40(d + d ) where D is distance (in centimeters) and d is stem diameter (in centimeters diameter at breast height). F. brayleyana trees surrounded by A. cunninghamii trees experience negligible competition, whereas A. cunninghamii surrounded by F. brayleyana trees suffer strong competition. • Conclusion: Forty times diameter offers a useful guide to the extent of competition in even-aged stands planted with these species. Competition can be observed empirically when pairs of trees are closer than 40 times the sum of their diameters, but the intensity of the competition may vary considerably with species