Assessing changes in vascular permeability in a hamster model of viral hemorrhagic fever

<p>Abstract</p> <p>Background</p> <p>A number of RNA viruses cause viral hemorrhagic fever (VHF), in which proinflammatory mediators released from infected cells induce increased permeability of the endothelial lining of blood vessels, leading to loss of plasma volume, hypotension, multi-organ failure, shock and death. The optimal treatment of VHF should therefore include both the use of antiviral drugs to inhibit viral replication and measures to prevent or correct changes in vascular function. Although rodent models have been used to evaluate treatments for increased vascular permeability (VP) in bacterial sepsis, such studies have not been performed for VHF.</p> <p>Results</p> <p>Here, we use an established model of Pichinde virus infection of hamsters to demonstrate how changes in VP can be detected by intravenous infusion of Evans blue dye (EBD), and compare those measurements to changes in hematocrit, serum albumin concentration and serum levels of proinflammatory mediators. We show that EBD injected into sick animals in the late stage of infection is rapidly sequestered in the viscera, while in healthy animals it remains within the plasma, causing the skin to turn a marked blue color. This test could be used in live animals to detect increased VP and to assess the ability of antiviral drugs and vasoactive compounds to prevent its onset. Finally, we describe a multiplexed assay to measure levels of serum factors during the course of Pichinde arenavirus infection and demonstrate that viremia and subsequent increase in white blood cell counts precede the elaboration of inflammatory mediators, which is followed by increased VP and death.</p> <p>Conclusions</p> <p>This level of model characterization is essential to the evaluation of novel interventions designed to control the effects of virus-induced hypercytokinemia on host vascular function in VHF, which could lead to improved survival.</p

Global urban environmental change drives adaptation in white clover.

Urbanization transforms environments in ways that alter biological evolution. We examined whether urban environmental change drives parallel evolution by sampling 110,019 white clover plants from 6169 populations in 160 cities globally. Plants were assayed for a Mendelian antiherbivore defense that also affects tolerance to abiotic stressors. Urban-rural gradients were associated with the evolution of clines in defense in 47% of cities throughout the world. Variation in the strength of clines was explained by environmental changes in drought stress and vegetation cover that varied among cities. Sequencing 2074 genomes from 26 cities revealed that the evolution of urban-rural clines was best explained by adaptive evolution, but the degree of parallel adaptation varied among cities. Our results demonstrate that urbanization leads to adaptation at a global scale

Habitus, work and contributive justice

The article explores the benefits of relating Bourdieu’s critical analysis of inequalities and domination to the theory of contributive justice. The latter is a normative theory concerning divisions of labour between jobs of different qualities that provide their holders with unequal possibilities for realizing their potential. Both approaches have Aristotelian influences in their emphasis on the development of dispositions and abilities through practice. It is argued that while this theory needs to consider the shaping of the habitus in early life prior to entry into the labour market, the concept of the unequal division of labour highlights a key structuring force of the social field. In so doing it makes explicit some justifications for Bourdieu’s critique of symbolic domination and the struggles of the social field that are left largely implicit in his work

'Con-viviality' and beyond: identity dynamics in a young men's prison

This chapter explores the configuration of identity, social relations and ethnicity within the confines of a young men’s prison. The site of intense deprivations, referred to by Sykes (1958) as the ‘pains of imprisonment’, prisons gather together many of those people also bearing the pains of structural disenfranchisement and marginalisation which characterise deprived neighbourhoods (Wacquant, 2007)