Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations

A competitive CZE method for quality control analysis of multivitamin preparations and veterinary products containing B-group vitamins was developed. Vitamins of interest are thiamine hydrochloride (B1), thiamine monophosphate chloride (B1a), riboflavine (B2), riboflavine-5′monophosphate (B2a), nicotinamide (B3), d-pantothenic acid calcium salt (B5), pyridoxine hydrochloride (B6), folic acid (B9), and 4-aminobenzoic acid (B10). These analytes were separated optimizing the experimental conditions in 20 mM tetraborate buffer pH = 9.2 as a BGE (background electrolyte), on a Beckman P/ACE System MDQ instrument, using uncoated fused silica capillary. The effective capillary length was of 49.5 cm, I.D. = 50 μm, the applied voltage 20 kV and the temperature 25°C. Detection was performed by a diode array detector at 214 nm for all vitamins except B5 (190 nm) and B2a (260 nm). Separation time was about 9 min. After experimental conditions optimization, the proposed method was validated. Precision of migration time and corrected peak area, linearity range, LOD and LOQ, accuracy (recovery), robustness, and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of the pharmaceutical real samples were performed and confirmed the versatility of this method

rapid and sensitive cze method for quality control analysis of pharmaceuticals containing pseudoephedrine triprolidine and paracetamol

A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds: Pseudoephedrine hydrochloride (PSE), Triprolidine hydrochloride (TRI) and Paracetamol (PAR) were separated and quantitatively determined using the tris-borate 30 mM buffer at pH = 9.0 as a Background Electrolyte (BGE). The electrophoretic separation was carried out at 25 kV in an unmodified fused silica capillary of I.D. = 50 μm with a "bubble-cell" for UV detection at 210 nm and 25°C. The separation was reached in about 3 min. After calibration the method was applied for analysis of three commercially available pharmaceutical preparations. The repeatability (RSD%) of migration time (tm) was ranging between 0.47% and 0.90% and of peak areas (A) between 0.63% and 3.64%. The Limit of Detection (LOD) values was of 0.19 μg/mL, 0.31 μg/mL and 0.08 μg/mL for respectively PSE, TRI and PAR. The results obtained in this study showed that the proposed method was useful in routinely analysis of pharmaceuticals

Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations

A competitive CZE method for quality control analysis of multivitamin preparations and veterinary products containing B-group vitamins was developed. Vitamins of interest are thiamine hydrochloride (B 1 ), thiamine monophosphate chloride (B 1a ), riboflavine (B 2 ), riboflavine-5 monophosphate (B 2a ), nicotinamide (B 3 ), d-pantothenic acid calcium salt (B 5 ), pyridoxine hydrochloride (B 6 ), folic acid (B 9 ), and 4-aminobenzoic acid (B 10 ). These analytes were separated optimizing the experimental conditions in 20 mM tetraborate buffer pH = 9.2 as a BGE (background electrolyte), on a Beckman P/ACE System MDQ instrument, using uncoated fused silica capillary. The effective capillary length was of 49.5 cm, I.D. = 50 µm, the applied voltage 20 kV and the temperature 25 • C. Detection was performed by a diode array detector at 214 nm for all vitamins except B 5 (190 nm) and B 2a (260 nm). Separation time was about 9 min. After experimental conditions optimization, the proposed method was validated. Precision of migration time and corrected peak area, linearity range, LOD and LOQ, accuracy (recovery), robustness, and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of the pharmaceutical real samples were performed and confirmed the versatility of this method

CZE determination of somatostatin in pharmaceutical preparations

We propose a simple and accurate method for CE quantitative determination of somatostatin in pharmaceutical preparations. The method is specific for somatostatin as indicated by the resolution between the analyte and the analogue peptides which differ from somatostatin by one aminoacid. The linearity range is from 0.02 to 0.35 mg/ml. The recovery of the somatostatin from a pharmaceutical product is about 100.0%. (C) 2000 Elsevier Science B.V. All rights reserved

Development and Validation of a MEKC Method for Determination of Nitrogen-Containing Drugs in Pharmaceutical Preparations

A fast and accurate micellar electrokinetic capillary chromatography method was developed for quality control of pharmaceutical preparations containing cold remedies as acetaminophen, salicylamide, caffeine, phenylephrine, pseudoephedrine, norephedrine and chlorpheniramine. The method optimization was realized on a Beckman P/ACE System MDQ instrument. The baseline separation of seven analytes was performed in an uncoated fused silica capillary internal diameter (ID)=50 µm using tris-borate (20 mM, pH=8.5) containing sodium dodecyl sulphate 30 mM BGE. On line-UV detection at 214 nm was performed and the applied voltage was 10 kV. The operating temperature was 25°C. After experimental conditions optimization, the proposed method was validated. The evaluated parameters were: precision of migration time and of corrected peak area ratio, linearity range, limit of detection, limit of quantification, accuracy (recovery), ruggedness and applicability. The method was then successfully applied for the analysis of three pharmaceutical preparations containing some of the analytes listed before

Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations.

A competitive CZE method for quality control analysis of multivitamin preparations and veterinary products containing B-group vitamins was developed. Vitamins of interest are thiamine hydrochloride (B(1)), thiamine monophosphate chloride (B(1a)), riboflavine (B(2)), riboflavine-5'monophosphate (B(2a)), nicotinamide (B(3)), d-pantothenic acid calcium salt (B(5)), pyridoxine hydrochloride (B(6)), folic acid (B(9)), and 4-aminobenzoic acid (B(10)). These analytes were separated optimizing the experimental conditions in 20 mM tetraborate buffer pH = 9.2 as a BGE (background electrolyte), on a Beckman P/ACE System MDQ instrument, using uncoated fused silica capillary. The effective capillary length was of 49.5 cm, I.D. = 50 μm, the applied voltage 20 kV and the temperature 25°C. Detection was performed by a diode array detector at 214 nm for all vitamins except B(5) (190 nm) and B(2a) (260 nm). Separation time was about 9 min. After experimental conditions optimization, the proposed method was validated. Precision of migration time and corrected peak area, linearity range, LOD and LOQ, accuracy (recovery), robustness, and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of the pharmaceutical real samples were performed and confirmed the versatility of this method

The solubility of lead(II) deoxycholate.

Lead(II) deoxycholate solubility has been studied as a function of deoxycholate concentration at 25°C in N(CH3)4Cl as constant ionic medium at seven different concentrations. The solubility product and the association constant have been determined in all cases. On the basis of these values the use of an 3electrode to measurethe free concentration of deoxycholate is proposed. From the value of Ks (solubility product) of lead(II) deoxycholate, the amperometric titration of deoxycholate by means of standard lead(II) can be planned

Aspartate and Glutamate as ligand towards lead(II).

The complexs formation between lead(II) and aspartate or glutamate has bees studied by measuring the electromotive force of galvanic cells containing glass and stationary diluited amalgam electrodes at 25°C and in 1.00M NaClO4. Experimental data have been explained by assuming the presence of species PbL, PbHL, PbH2L, PbL2,PbH2L2; PbH3L2 and PbH4L2 where L indicates aspartate or glutamate. The stability constants of each complex have been determined showing that protonated species prevail in a wide range of coincentration of reagents. Results have been compared with those obtained for glycine and serine