Polymethyloctilsiloxane Adsorbed On Porous Silica As A Packing Material For Reversed Phase High Performance Liquid Chromatography

Packing material having 10, 20, 30, 40 and 50% loadings of polymethyloctilsiloxane on porous silica particles have been prepared and tested. Solvent extraction tests, measurements of physical properties and determinations of chromatographic parameters show that an initial loading of 40% gives a packing material with satisfactory chromatographic properties.Packing material having 10, 20, 30, 40 and 50% loadings of polymethyloctilsiloxane on porous silica particles have been prepared and tested. Solvent extraction tests, measurements of physical properties and determinations of chromatographic parameters show that an initial loading of 40% gives a packing material with satisfactory chromatographic properties.1761265127

Stability Of High-performance Liquid Chromatography Columns Packed With C1 And C8 Polysiloxanes Sorbed Into Porous Silica Particles

Columns, packed with 10 μm diameter porous silica particles (15 nm diameter pores) containing polydimethylsiloxane (20%, w/w) or polymethyloctylsiloxane (40%, w/w) sorbed into the pores, were tested for chromatographic stability as a function of elution volume. No significant changes in efficiency, retention factor or separation factor were observed following extended washing (up to 5000 column volumes) of either stationary phase with methanol-water mobile phases. © 1995 Elsevier Science B.V. All rights reserved.6971-2159164Martin, Synge, A new form of chromatogram employing two liquid phases: A theory of chromatography. 2. Application to the micro-determination of the higher monoamino-acids in proteins. (1941) Biochem J, 35, p. 1358Snyder, Kirkland, (1979) Introduction to Modern Liquid Chromatography, p. 323. , 2nd ed., Wiley, New York, Ch. 8Crombeen, Heemsta, Kraak, (1984) J. Chromatogr., 286, p. 119Hansen, Helboe, Thomsen, (1991) J. Chromatogr., 544, p. 53Schmit, (1971) Modern Practice of Liquid Chromatography, p. 375. , J.J. Kirkland, Wiley, New York, Ch. 11Anazawa, Jardim, (1994) J. Liq. Chromatogr., 17, p. 126