Uncertainty Quantification for Atlas-Level Cell Type Transfer

Single-cell reference atlases are large-scale, cell-level maps that capture cellular heterogeneity within an organ using single cell genomics. Given their size and cellular diversity, these atlases serve as high-quality training data for the transfer of cell type labels to new datasets. Such label transfer, however, must be robust to domain shifts in gene expression due to measurement technique, lab specifics and more general batch effects. This requires methods that provide uncertainty estimates on the cell type predictions to ensure correct interpretation. Here, for the first time, we introduce uncertainty quantification methods for cell type classification on single-cell reference atlases. We benchmark four model classes and show that currently used models lack calibration, robustness, and actionable uncertainty scores. Furthermore, we demonstrate how models that quantify uncertainty are better suited to detect unseen cell types in the setting of atlas-level cell type transfer.Comment: Workshop paper at the 2022 ICML Workshop on Computational Biolog

Agent for preventing and ameliorating aging of skin

PROBLEM TO BE SOLVED: To obtain the subject new cosmetic by compounding a phosphorylated polysaccharide having humectant action and collagen-producing performance as an active component. SOLUTION: This cosmetic contains a compound of the formula (Glc is glucose residue; Gal is galactose residue; Rha is rhamnose residue; (m) is 0-3; (n) is 1,000-5,000) as an active component. The component of the formula can be produced by hydrolyzing actinase E of defatted milk, treating the defatted milk with ultrafiltration membrane, sterilizing the filtrate to obtain a culture medium, aseptically pipetting the sterilized medium into a jar fermenter, inoculating a precultured liquid, culturing at 20 deg.C and pH5.5 using NH3 water as a neutralizing agent, subjecting the cultured product to centrifugal separation after culture to recover the supernatant, adding an equal amount of CH3 OH, recovering the produced precipitate, dissolving the precipitate in 0.2N saline water, repeating the precipitation operation with CH3 OH, subjecting the treated precipitate to electrophoresis, recovering the component impregnated into the gel and purifying the recovered fraction by ion-exchange chromatography to obtain purified polysaccharide. It is necessary to compound a cosmetic with &gt;=0.001wt.% of the compound of the formula.</p

SOY MILK FERMENTATION

The present invention relates to a method for preparing a soy-based fermented food product using L. delbrueckii and a strain capable of stimulating growth of L. delbrueckii, and a food product obtainable by such method. The invention also provides for a culture comprising said strains, and the use thereof for preparing said fermented food product.</p