EFFECT OF BLUMEA LACERA ON TISSUE GSH, LIPID PEROXIDATION AND HEPATIC CELLS IN ETHANOL INDUCED HEPATOTOXICITY IN RATS

Objective: To evaluate hepatoprotective effects of ethanol extract of aerial part of Blumea lacera (BLEE) against ethanol-induced hepatotoxicity in rats. Methods: The in vivo antioxidant activity of BLEE was assessed by determining the tissue glutathione (GSH) and lipid peroxidation (LPO) levels. The BLEE at the doses of 200 and 400 mg/kg and silymarin 100 mg/kg administered to the ethanol challenged rats. The effects of BLEE and silymarin on Physical and Biochemical Parameters were measured. Similarly, histopathological changes of the liver were studied. Results: The BLEE showed in vivo antioxidant activity. A significant (P<0.001) decrease in SGOT, SGPT, ALP, total and direct bilirubin was observed in BLEE treated group at doses i.e. 200 mg/kg and 400 mg/kg as compared to intoxicated group. Liver damage in animal pretreated with BLEE was minimal with distinct preservation of structures and the architectural frame of the hepatic cells. Conclusion: These findings demonstrated the hepatoprotective effects of BLEE against ethanol-induced liver damage

IN VIVO ANTIOXIDANT AND HEPATOPROTECTIVE EFFECTS OF HUGONIA MYSTAX IN PARACETAMOL INDUCED HEPATOTOXICITY IN RATS.

Objectives: The objective of the present work is to study the in vivo anti-oxidant and hepatoprotective effects of Hugonia mystax in paracetamol (PCM)induced hepatotoxicity in rats.Methods: The in vivo anti-oxidant activity of 70% ethanol extract of leaves of H. mystax (HMEE) was assessed by determining the tissue glutathioneand lipid peroxidation (LPO) levels. HMEE 200 and 400 mg/kg p.o. doses and silymarin p.o.100 mg/kg were administered to the PCM challengedrats. The effect of HMEE and silymarin on physical (liver weight and liver volume) and biochemical parameters (serum enzymes [serum glutamicoxaloacetic transaminase (SGOT) and serum glutamate-pyruvate transaminase (SGPT)], alkaline phosphate [ALP], and bilirubin) were measured.Furthermore, histopathological changes in the liver were studied.Results: The HMEE showed in the vivo anti-oxidant activity. Pre-treatment with HMEE for 7 days significantly reduced the elevated biochemicalparameters (SGOT, SGPT, ALP, and bilirubin levels). The hepatic damage in animal pretreated with HMEE was minimal with distinct preservation ofstructures and architectural frame of the hepatic cells.Conclusion: These findings demonstrate the protective nature of HMEE against PCMKeywords: Hugonia mystax, Hepatoprotective, In vivo anti-oxidant, Paracetamol

Antidiarrheal Activity of Albizzia lebbeck Leaves

Worldwide, more than 5–8 million deaths are caused each year by diarrhea. At present, used anti-diarrheal drugs show adverse effects in some form. Hence, there is a need to explore natural drugs as alternative therapy. Preliminary phytochemical screening, Acute oral toxicity and antidiarrheal activity of 70% ethanolic extract of the Albizzia lebbeck leaves (ALL) were performed. ALL were assessed for antidiarrheal activity using castor oil, Prostaglandin-E2 (PGE2) and intestinal motility induced diarrhea in rats. On the basis of acute oral toxicity, 100 and 200 mg/kg body weight doses were selected for antidiarrheal activity. Preliminary phytochemical screenings revealed presence of flavonoids tannins and saponins in extract. ALL showed significant inhibitory activity against castor oil, PGE2 induced diarrhea and intestinal motility. On the basis of the result, it can be concluded that the ALL possess significant antidiarrheal activity