Soil chemical attributes under west indian cherry fertigated with nitrogen and humic substances

The use of humic substances (HS) in the soil can increase the efficiency of nitrogen fertilizers and contribute to the increment in soil organic matter and plant nutrient availability. Hence, this study aimed to evaluate, in two production cycles, the effect of applying HS and different nitrogen (N) doses on the chemical attributes and organic matter fractions of a soil cultivated with West Indian cherry in the Brazilian semiarid region. The experiment was set up in split plots, arranged in strips with four replicates. The absence or presence of HS using KS100 as the source was tested in the plots, and N fertilization (50; 75; 100; 125 and 150% of the recommended dose), using urea, was tested in the subplots. Soil pH, H+Al, Al3+, and Na+, K+, P, Ca2+, Mg2+, Fe2+, Mn2+ and Zn2+ contents were determined, and cation exchange capacity and bases saturation (V %) in the 0-0.2 and 0.2-0.4 m layers were calculated. Analyses of stocks of total organic carbon and carbon (C) of the humic acid, fulvic acid and humin fractions, and humic substances were performed. It was possible to observe that, in the second production cycle of West Indian cherry, the soils showed higher contents of nutrients available to plants. Increase in N availability did not enable a clear trend in the behavior of chemical attributes and organic C stocks in the soil. Under the studied conditions, KS100 application allowed a reduction in the C stocks of the HS of the soil

Acute Toxicity and Cytotoxicity Effect of Ethanolic Extract of Spondias tuberosa Arruda Bark: Hematological, Biochemical and Histopathological Evaluation

ABSTRACT Spondias tuberosa Arruda, popularly named as umbu, is native from savanna-like vegetation and widely used for medicinal purposes, however, the toxicological profile is not available yet. This study evaluated the phytochemical profile and acute toxicity and citoxicity of Ethanolic Extract of Spondias tuberosa Arruda Bark (EEStb) in hematological, biochemical and histopathological parameters. Female Wistar rats were divided into: control (C) and animal treated single doses of 300mg/Kg (EEStb300) or 2.000mg/kg body weight (ESStb2.000) of the EEStb. After 24 hours and 14 days from gavage, the behavior, hematological, biochemical and histopathological parameters were assayed. Cytotoxicity effect was evaluated on HEp-2 cell lines. Neither EEStb300 nor EEStb2.000 produced mortality nor changes in body weight during the 14-days of observation, but EEStb2.000 reduced quietly the food and water intake as well as locomotor activity at first day. There were no changes in macroscopic, histopathological, biochemical and hematological parameters. EEStb in concentrations of 6.25- 50μg ml−1 on HEp-2 cell did not produce cytotoxic effect. These results suggest that EEStb did not cause acute toxicity and cytotoxic, suggesting a good safety rate for Spondias tuberosa Arruda