AbstractIn this paper we continue the investigation on the relation between the behaviour of the adjoint of a C0-semigroup and the structure of the underlying Banach space. The following results are proved: if X lacks the RNP then Xβ*/X is nonseparable, and if X* lacks the RNP then either X*/Xβ or Xββ/X is nonseparable. The results are applied to obtain a trichotomy theorem for adjoint semigroups. Also some applications to C0-groups are given