Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples

Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for species-specific identification of C. jejuni, C. coli, C. lari, C. upsaliensis and of a hipO gene nucleotide probe for detection of C. jejuni by colony-blot hybridization were determined by testing a total of 75 reference strains of Campylobacter spp. and related organisms. The rt-PCR method allowed species-specific detection of Campylobacter spp. in naturally infected pig faecal samples after an enrichment step, whereas the hybridization approach enhanced the specific isolation of C. jejuni (present in minority to C. coli) from pigs. Conclusions: The rt-PCR was specific for Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis and the colony-blot hybridization approach provided an effective tool for isolation of C. jejuni from pig faecal samples typically dominated by C. coli. Significance and impact of study: Species differentiation between thermophilic Campylobacter is difficult by phenotypic methods and the developed rt-PCR provides an easy and fast method for such differentiation. Detection of C. jejuni by colony hybridization may increase the isolation rate of this species from pig feces

CP violation in 5D Split Fermions Scenario

We give a new configuration of split fermion positions in one extra dimension with two different Yukawa coupling strengths for up-type, $h_u$, and down-type, $h_d$, quarks at $\frac{h_u}{h_d}=36.0$. The new configurations can give enough CP violating (CPV) phase for accommodating all currently observed CPV processes. Therefore, a 5D standard model with split fermions is viable. In addition to the standard CKM phase, new CPV sources involving Kaluza-Klein(KK) gauge bosons coupling which arise from the fact that unitary rotation which transforms weak eigenstates into their mass eigenstates only holds for the zero modes which are the SM fields and not for the KK excitations. We have examined the physics of kaon, neutron, and $B/D$ mesons and found the most stringent bound on the size $R$ of the extra dimension comes from $|\epsilon_K|$. Moreover, it depends sensitively on the width, $\sigma$, of the Gaussian wavefunction in the extra dimension used to describe of the fermions. When $\sigma/R \ll 1$, the constraint will be lifted due to GIM suppression on the flavor changing neutral current(FCNC) and CPV couplings.Comment: 24 pages, 8 figure

Recommended Finite Element Formulations for the Analysis of Off-shore Blast Walls in an Explosion

This study suggests relevant finite element (FE) formulations for the structural analysis of offshore blast walls subjected to blast loadings due to hydrocarbon explosions. The present blast wall model adopted from HSE (2003) consists of a corrugated panel and supporting members, and was modelled with shell, thick-shell, and solid element combinations in LS-DYNA, an explicit finite element analysis (FEA) solver. Stainless and mild steels were employed as materials for the blast wall model, with consideration of strain rate effect throughout ten (10) pulse pressure load regimes. The obtained FEA results were validated by experimental data from HSE (2003) with decent agreement. In the present study, recommended FE formulations with additional hourglass control functions were widely discussed from the perspectives of solution accuracy and computational cost based on a statistical approach. The obtained outcomes could be used for the structural analysis and design of offshore blast walls in the estimations of maximum and permanent deformations under blast loadings.111Ysciescopu

Rational Symplectic Field Theory for Legendrian knots

We construct a combinatorial invariant of Legendrian knots in standard contact three-space. This invariant, which encodes rational relative Symplectic Field Theory and extends contact homology, counts holomorphic disks with an arbitrary number of positive punctures. The construction uses ideas from string topology.Comment: 58 pages, many figures; v3: minor corrections; final version, to appear in Inventiones Mathematica

A kinetic and kinematic analysis of the Harai-goshi judo technique

The purpose of this study was to analyze and compare the kinetic and kinematic characteristics of the throwing technique, Harai-goshi of novice and advanced judo competitors. A heterogeneous group of 28 male and female judo competitors participated in this study. Each subject was required to perform three successful trials. Kinetic and kinematic data were collected by utilizing the Kistler Instrument Corporation Multicomponent Force Measuring Platform System and the Peak Technologies Motion Video Analysis System. Data were collected in order to quantitatively and qualitatively analyze the horizontal and vertical forces of the support leg, and the horizontal and vertical velocities of the sweeping leg during execution of the throwing technique, Harai-goshi (hip sweep). The study revealed a significant difference in horizontal force application, between novice and advanced judo competitors. In addition, we found numerous significant relationships among mean horizontal ground reaction force application and horizontal leg sweep velocity in 19 of the 28 participants when analyzed individually. The results suggest (1) leg sweep velocity is a function of ground reaction force application; and (2) horizontal leg sweep velocity plays a primary role in good technical execution of the Harai-goshi throw

SSB taxes and diet quality in US preschoolers: estimated changes in the 2010 Healthy Eating Index

Background: Taxing sugar-sweetened beverages (SSBs) has been proposed as a strategy to combat child obesity. Yet it is unclear how a tax on SSBs might influence the overall quality of diet in preschool children. Thus, we use simulated price increases and the 2010 Healthy Eating Index (HEI-2010) to explore the relationship between SSB taxes and diet quality in preschool children. Methods: Price and purchase data from the 2009–2012 Nielsen Homescan Panel and a two-part marginal effects model were used to estimate relative changes in purchases with a 20% increase in the price of SSBs. Demand elasticities were applied to dietary intake data for children ages 2–5 years from the National Health and Nutrition Examination Survey (2009–2010 and 2011–2012) to estimate the impact of a 20% SSB tax on dietary intake and quality (HEI-2010). Results: A 20% increase in the price of SSBs was associated with lower total caloric intake (−28 kcal d−1, p < 0.01), caloric intake from juice drinks (−20 kcal d−1, p < 0.01), added sugars (−4.1 servings d−1, p = 0.03), refined grains (−0.63 servings d−1, p < 0.01) and total meat (−0.56 servings d−1, p < 0.01). Beneficial decreases in empty calories and refined grains were offset by unfavourable changes in fatty acid profile, total protein, vegetables and fruit, such that total HEI scores (0–100 range) were not meaningfully changed with a 20% increase in SSB price (difference: −0.85, p < 0.01). Conclusions: A 20% tax on SSBs could decrease caloric intake, and intakes of added sugars and SSBs, but may not improve diet quality as an isolated intervention among US preschool children

INTEGRAL observations of TeV plerions

Amongst the sources seen in very high gamma-rays several are associated with Pulsar Wind Nebulae (``TeV plerions''). The study of hard X-ray/soft gamma-ray emission is providing an important insight into the energetic particle population present in these objects. The unpulsed emission from pulsar/pulsar wind nebula systems in the energy range accessible to the INTEGRAL satellite is mainly synchrotron emission from energetic and fast cooling electrons close to their acceleration site. Our analyses of public INTEGRAL data of known TeV plerions detected by ground based Cherenkov telescopes indicate a deeper link between these TeV plerions and INTEGRAL detected pulsar wind nebulae. The newly discovered TeV plerion in the northern wing of the Kookaburra region (G313.3+0.6 powered by the middle aged PSR J1420-6048) is found to have a previously unknown INTEGRAL counterpart which is besides the Vela pulsar the only middle aged pulsar detected with INTEGRAL. We do not find an INTEGRAL counterpart of the TeV plerion associated with the X-ray PWN ``Rabbit'' G313.3+0.1 which is possibly powered by a young pulsar.Comment: 4 pages, 6 figures, proceedings of conference "The Multi-Messenger Approach to High-Energy Gamma-ray Sources" Barcelona/Spain (2006

Magnetic ordering in Sr2RuO4 induced by nonmagnetic impurities

We report unusual effects of nonmagnetic impurities on the spin-triplet superconductor Sr2RuO4. The substitution of nonmagnetic Ti4+ for Ru4+ induces localized-moment magnetism characterized by unexpected Ising anisotropy with the easy axis along the interlayer c direction. Furthermore, for x(Ti) > 0.03 magnetic ordering occurs in the metallic state with the remnant magnetization along the c-axis. We argue that the localized moments are induced in the Ru4+ and/or oxygen ions surrounding Ti4+ and that the ordering is due to their interaction mediated by itinerant Ru-4d electrons with strong spin fluctuations.Comment: 5 pages, 4figure

Quantized adiabatic charge pumping and resonant transmission

Adiabatically pumped charge, carried by non-interacting electrons through a quantum dot in a turnstile geometry, is studied as function of the strength of the two modulating potentials (related to the conductances of the two point-contacts to the leads) and of the phase shift between them. It is shown that the magnitude and sign of the pumped charge are determined by the relative position and orientation of the closed contour traversed by the system in the parameter plane, and the transmission peaks (or resonances) in that plane. Integer values (in units of the electronic charge $e$) of the pumped charge (per modulation period) are achieved when a transmission peak falls inside the pumping contour. The integer value is given by the winding number of the pumping contour: double winding in the same direction gives a charge of 2, while winding around two opposite branches of the transmission peaks or winding in opposite directions can give a charge close to zero.Comment: 7 pages, 12 figure

Transport in Coupled Quantum Dots: Kondo Effect Versus Anti-Ferromagnetic Correlation

The interplay between the Kondo effect and the inter-dot magnetic interaction in a coupled-dot system is studied. An exact result for the transport properties at zero temperature is obtained by diagonalizing a cluster, composed by the double-dot and its vicinity, which is connected to leads. It is shown that the system goes continuously from the Kondo regime to an anti-ferromagnetic state as the inter-dot interaction is increased. The conductance, the charge at the dots and the spin-spin correlation are obtained as a function of the gate potential.Comment: 4 pages, 3 postscript figures. Submitted to PR