Isolation and pathogenicity of Phytophthora species from declining Rubus anglocandicans

Rubus anglocandicans is the most widespread and abundant blackberry species within the European blackberry (Rubus fruticosus) aggregate in Western Australia (WA). European blackberry is also one of the 32 Weeds of National Significance in Australia. A disease recorded as 'blackberry decline' was first observed in some blackberry sites in WA in 2006. A disease survey was conducted in the Manjimup-Pemberton region along the Warren and Donnelly River catchments in WA between 2010 and 2012. Phytophthora amnicola, P. bilorbang, P. cryptogea, P. inundata, P. litoralis, P. multivora, P. taxon personii, P. thermophila and a P. thermophila × amnicola hybrid were recovered from declining and adjacent decline-free sites, as well as from streams and rivers. Phytophthora cinnamomi was isolated from dying Banksia and Eucalyptus species from two non-decline sites. Of these species, P. bilorbang and P. cryptogea were more pathogenic than the others in under-bark inoculations using excised stems (primocanes), in planta primocane inoculations in blackberry growing wild in native forest stands, and in glasshouse pot trials. It was concluded that blackberry decline is a complex syndrome and Phytophthora species, in particular P. bilorbang and P. cryptogea, together with temporary inundation, are major biotic and abiotic factors contributing to blackberry decline

Duality in the Quantum Hall Effect - the Role of Electron Spin

At low temperatures the phase diagram for the quantum Hall effect has a powerful symmetry arising from the Law of Corresponding States. This symmetry gives rise to an infinite order discrete group which is a generalisation of Kramers-Wannier duality for the two dimensional Ising model. The duality group, which is a subgroup of the modular group, is analysed and it is argued that there is a quantitative difference between a situation in which the spin splitting of electron energy levels is comparable to the cyclotron energy and one in which the spin splitting is much less than the cyclotron energy. In the former case the group of symmetries is larger than in the latter case. These duality symmetries are used to constrain the scaling functions of the theory and, under an assumption of complex meromorphicity, a unique functional form is obtained for the crossover of the conductivities between Hall states as a function of the external magnetic field. This analytic form is shown to give good agreement with experimental data. The analysis requires a consideration of the way in which longitudinal resistivities are extracted from the experimentally measured longitudinal resistances and a novel method is proposed for determining the correct normalisation for the former.Comment: 22 pages, 8 figures, typeset in LaTe

Fibulin-1 Is Increased in Asthma – A Novel Mediator of Airway Remodeling?

Background: The extracellular matrix is a dynamic and complex network of macromolecules responsible for maintaining and influencing cellular functions of the airway. The role of fibronectin, an extracellular matrix protein, is well documented in asthma. However, the expression and function of fibulin-1, a secreted glycoprotein which interacts with fibronectin, has not been reported. Fibulin-1 is widely expressed in basement membranes in many organs including the lung. There are four isoforms in humans (A-D) of which fibulin-1C and 1D predominate. The objective of this study was to study the expression of fibulin-1 in volunteers with and without asthma, and to examine its function in vitro. Methodology/Principal Findings: We used immunohistochemistry and dot-blots to examine fibulin-1 levels in bronchial biopsies, bronchoalveolar lavage fluid and serum. Real-time PCR for fibulin-1C and 1D, and ELISA and western blotting for fibulin-1 were used to study the levels in airway smooth muscle cells. The function of fibulin-1C was determined by assessing its role, using an antisense oligonucleotide, in cell proliferation, migration and wound healing. A murine model of airway hyperresponsiveness (AHR) was used to explore the biological significance of fibulin-1. Levels of fibulin-1 were significantly increased in the serum and bronchoalveolar lavage fluid of 21 asthmatics compared with 11 healthy volunteers. In addition fibulin-1 was increased in asthma derived airway smooth muscle cells and fibulin-1C contributed to the enhanced proliferation and wound repair in these cells. These features were reversed when fibulin-1C was suppressed using an antisense oligomer. In a mouse model of AHR, treatment with an AO inhibited the development of AHR to methacholine. Conclusions: Our data collectively suggest fibulin-1C may be worthy of further investigation as a target for airway remodeling in asthma

Current and projected global distribution of Phytophthora cinnamomi, one of the world's worst plant pathogens

Globally, Phytophthora cinnamomi is listed as one of the 100 worst invasive alien species and active management is required to reduce impact and prevent spread in both horticulture and natural ecosystems. Conversely, there are regions thought to be suitable for the pathogen where no disease is observed. We developed a climex model for the global distribution of P. cinnamomi based on the pathogen's response to temperature and moisture and by incorporating extensive empirical evidence on the presence and absence of the pathogen. The climex model captured areas of climatic suitability where P. cinnamomi occurs that is congruent with all available records. The model was validated by the collection of soil samples from asymptomatic vegetation in areas projected to be suitable by the model for which there were few records. DNA was extracted, and the presence or absence of P. cinnamomi was determined by high-throughput sequencing (HTS). While not detected using traditional isolation methods, HTS detected P. cinnamomi at higher elevations in eastern Australia and central Tasmania as projected by the climex model. Further support for the climex model was obtained using the large data set from south-west Australia where the proportion of positive records in an area is related to the Ecoclimatic Index value for the same area. We provide for the first time a comprehensive global map of the current P. cinnamomi distribution, an improved climex model of the distribution, and a projection to 2080 of the distribution with predicted climate change. This information provides the basis for more detailed regional-scale modelling and supports risk assessment for governments to plan management of this important soil-borne plant pathogen

Thermodynamics of an Anyon System

We examine the thermal behavior of a relativistic anyon system, dynamically realized by coupling a charged massive spin-1 field to a Chern-Simons gauge field. We calculate the free energy (to the next leading order), from which all thermodynamic quantities can be determined. As examples, the dependence of particle density on the anyon statistics and the anyon anti-anyon interference in the ideal gas are exhibited. We also calculate two and three-point correlation functions, and uncover certain physical features of the system in thermal equilibrium.Comment: 18 pages; in latex; to be published in Phys. Rev.

Mitochondrial dysfunction contributes to the senescent phenotype of IPF lung fibroblasts

Increasing evidence highlights that senescence plays an important role in idiopathic pulmonary fibrosis (IPF). This study delineates the specific contribution of mitochondria and the superoxide they form to the senescent phenotype of lung fibroblasts from IPF patients (IPF-LFs). Primary cultures of IPF-LFs exhibited an intensified DNA damage response (DDR) and were more senescent than age-matched fibroblasts from control donors (Ctrl-LFs). Furthermore, IPF-LFs exhibited mitochondrial dysfunction, exemplified by increases in mitochondrial superoxide, DNA, stress and activation of mTORC1. The DNA damaging agent etoposide elicited a DDR and augmented senescence in Ctrl-LFs, which were accompanied by disturbances in mitochondrial homoeostasis including heightened superoxide production. However, etoposide had no effect on IPF-LFs. Mitochondrial perturbation by rotenone involving sharp increases in superoxide production also evoked a DDR and senescence in Ctrl-LFs, but not IPF-LFs. Inhibition of mTORC1, antioxidant treatment and a mitochondrial targeting antioxidant decelerated IPF-LF senescence and/or attenuated pharmacologically induced Ctrl-LF senescence. In conclusion, increased superoxide production by dysfunctional mitochondria reinforces lung fibroblast senescence via prolongation of the DDR. As part of an auto-amplifying loop, mTORC1 is activated, altering mitochondrial homoeostasis and increasing superoxide production. Deeper understanding the mechanisms by which mitochondria contribute to fibroblast senescence in IPF has potentially important therapeutic implications

Universal flow diagram for the magnetoconductance in disordered GaAs layers

The temperature driven flow lines of the diagonal and Hall magnetoconductance data (G_{xx},G_{xy}) are studied in heavily Si-doped, disordered GaAs layers with different thicknesses. The flow lines are quantitatively well described by a recent universal scaling theory developed for the case of duality symmetry. The separatrix G_{xy}=1 (in units e^2/h) separates an insulating state from a spin-degenerate quantum Hall effect (QHE) state. The merging into the insulator or the QHE state at low temperatures happens along a semicircle separatrix G_{xx}^2+(G_{xy}-1)^2=1 which is divided by an unstable fixed point at (G_{xx},G_{xy})=(1,1).Comment: 10 pages, 5 figures, submitted to Phys. Rev. Let

Quality of mother-child interaction, differences in sexual attitudes, and inter-generational disagreement on sexuality.

The current paper examines the frequency of inter-generational disagreement reported by mothers and adolescents as a function of the quality of their interaction, and the match between their sexual attitudes. We expected that the quality of family interaction would act as a "family asset" that would enable members of families to manage and control the tensions caused by differences in (sexual) attitudes. Data on 319 British adolescent-mother pairs were analysed using structural equation modelling, revealing good support for these expectations: differences in sexual attitudes were more strongly linked to inter-family disagreement in low quality of mother-child interaction families than in high quality of motherchild interaction families. Implications of the study are discussed. © 1997 OPA (Overseas Publishers Association)

Open strings in relativistic ion traps

Electromagnetic plane waves provide examples of time-dependent open string backgrounds free of $\alpha'$ corrections. The solvable case of open strings in a quadrupolar wave front, analogous to pp-waves for closed strings, is discussed. In light-cone gauge, it leads to non-conformal boundary conditions similar to those induced by tachyon condensates. A maximum electric gradient is found, at which macroscopic strings with vanishing tension are pair-produced -- a non-relativistic analogue of the Born-Infeld critical electric field. Kinetic instabilities of quadrupolar electric fields are cured by standard atomic physics techniques, and do not interfere with the former dynamic instability. A new example of non-conformal open-closed duality is found. Propagation of open strings in time-dependent wave fronts is discussed.Comment: 43 pages, 11 figures, Latex2e, JHEP3.cls style; v2: one-loop amplitude corrected, open-closed duality proved, refs added, miscellaneous improvements, see historical note in fil