Neuropilin-1 is upregulated in the adaptive response of prostate tumors to androgen-targeted therapies and is prognostic of metastatic progression and patient mortality

Recent evidence has implicated the transmembrane co-receptor neuropilin-1 (NRP1) in cancer progression. Primarily known as a regulator of neuronal guidance and angiogenesis, NRP1 is also expressed in multiple human malignancies, where it promotes tumor angiogenesis. However, non-angiogenic roles of NRP1 in tumor progression remain poorly characterized. In this study, we define NRP1\ud as an androgen-repressed gene whose expression is elevated during the adaptation of prostate tumors to androgen-targeted therapies (ATTs), and subsequent progression to metastatic castration-resistant prostate cancer (mCRPC). Using short hairpin RNA (shRNA)-mediated suppression of\ud NRP1, we demonstrate that NRP1 regulates the mesenchymal phenotype of mCRPC cell models and the invasive and metastatic dissemination of tumor cells in vivo. In patients, immunohistochemical staining of tissue microarrays and mRNA expression analyses revealed a positive association between NRP1 expression and increasing Gleason grade, pathological T score, positive lymph node status and primary therapy failure. Furthermore, multivariate analysis of several large clinical prostate cancer (PCa) cohorts identified NRP1 expression at radical prostatectomy as an independent prognostic biomarker of biochemical recurrence after radiation therapy, metastasis and cancer-specific mortality. This study identifies NRP1\ud for the first time as a novel androgen-suppressed gene upregulated during the adaptive response of prostate tumors to ATTs and a prognostic biomarker of clinical metastasis and lethal PCa

Conservation of grassland leafhoppers: A brief review

The leafhoppers, planthoppers and their allies (collectively known as the Auchenorrhyncha) are presented as a group of insects that are highly appropriate for studying grassland ecology and conservation, evaluating the conservation status of sites and monitoring environmental and habitat change. Semi-natural grasslands typically support dense populations and a wide range of species with diverse ecological strategies. Their numerical dominance in many grasslands means that they have considerable functional significance, both as herbivores and as prey for higher trophic levels. Population and assemblage studies are supported by good ecological knowledge about most species and modern identification keys. Hitherto, most studies have focused on the composition and structure of assemblages and how they are affected by conservation management. However, grasslands support many rare species with small and fragmented populations which deserve conservation attention in their own right, and recent work has started to reflect this. The effects of management on the composition and structure of grassland leafhopper populations and assemblages are described and an assessment is given of the main threats facing individual species and overall diversity. There is a need to synthesise the scattered literature on grassland leafhoppers, to provide a model for how the composition and structure of populations and assemblages respond to major environmental and anthropogenic gradients across large biogeographic areas. Such an analysis could help predict the impact of likely future changes in land use and climate