MÉCANISME DE LA PÉNÉTRATION ET VOIE D'ACHEMINEMENT DES GRAISSES DANS LA CELLULE ABSORBANTE DE L'INTESTIN GRÊLE DU PORC

International audienc

RÉPARTITION DES ACIDES GRAS INGÉRÉS ENTRE VAISSEAUX SANGUINS ET LACTÉALES CENTRALES DANS LE STROMA DES VILLOSITÉS

International audienc

Surgical-site infections and surgery of the salivary glands

SummaryObjectivesTo determine the surgical-site infection (SSI) incidence rate targeted on salivary gland surgery over a 2-year period (from January 2007 to December 2008). Then identify any risk factors associated with SSI in all the patients operated with no antibiotic prophylaxis in accordance with French Anesthesiology Society guidelines.Population and methodsNinety-three patients were operated during the standard SSI surveillance period. A case-control (one case for five controls) study was then conducted aiming to identify risk factors.ResultsThe SSI incidence rate was 9.7%. The case-control study failed to identify any relevant risk factor with univariate analysis.ConclusionAs no risk factors could be identified, we suggest that surgical antibioprophylaxis could be relevant in salivary glands surgery and should be evaluated in this setting

LA CELLULE ÉPITHÉLIALE ABSORBANTE DE L'INTESTIN GRÊLE DU PORC ULTRASTRUCTURE

International audienc

Scrambling free combinatorial labeling of alanine-β, isoleucine-δ1, leucine-proS and valine-proS methyl groups for the detection of long range NOEs.

International audienceSpecific isotopic labeling of methyl groups in proteins has greatly extended the applicability of solution NMR spectroscopy. Simultaneous labeling of the methyl groups of several different amino acid types can offer a larger number of useful probes that can be used for structural characterisations of challenging proteins. Herein, we propose an improved AILV methyl-labeling protocol in which L and V are stereo-specifically labeled. We show that 2-ketobutyrate cannot be combined with Ala and 2-acetolactate (for the stereo-specific labeling of L and V) as this results in co-incorporation incompatibility and isotopic scrambling. Thus, we developed a robust and cost-effective enzymatic synthesis of the isoleucine precursor, 2-hydroxy-2-(1'-[(2)H2], 2'-[(13)C])ethyl-3-keto-4-[(2)H3]butanoic acid, as well as an incorporation protocol that eliminates metabolic leakage. We show that application of this labeling scheme to a large 82 kDa protein permits the detection of long-range (1)H-(1)H NOE cross-peaks between methyl probes separated by up to 10 Å

An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular weight proteins

International audienceAn efficient synthetic route is proposed to produce 2-hydroxy-2-ethyl-3-oxobutanoate for the specific labelling of Ile methyl-γ2groups in proteins. The 2H, 13C-pattern of the biosynthetic precursor has been designed to optimize magnetization transfer, in large proteins, between these important structural probes and their corresponding backbone nuclei

A multicentric study of the G.E.T.T.E.C. (Groupe d’Etudes des Tumeurs de la Tête et du Cou) about 408 clinically complete responders after neo-adjuvant chemotherapy for head and neck carcinomas

info:eu-repo/semantics/publishe