502 research outputs found
Development of a novel tympanic temperature monitoring system for GT car racing athletes
Motor racing athletes, especially Grand Touring (GT) car racing drivers in the closed-cockpit category, can face lifethreatening situations caused by heat stroke. In this paper, a novel continuous tympanic temperature monitoring system, that could help to reduce this risk, is presented. The system consists of an earpiece containing an infrared-radiation-type tympanic thermometer and a micro-speaker. We validated the reliability of the system for tympanic temperature monitoring in 10 healthy volunteers (21.8 ± 1.0 S.D. years) using a temperature-controlled water bath. In addition, we evaluated the usefulness of the system with 2 professional drivers under real racing conditions in the 2010 SUPER GT International Series. The results showed strong correlation between the infrared-radiation tympanic temperature obtained by the present system and both the direct tympanic temperature (r = 0.994, n = 1119, P < 0.001) and the sublingual temperature (r = 0.972, n = 1119, P < 0.001) as a reference temperature. The mean difference between these temperatures was +0.09°C, and -0.08°C, and 95 % confidence interval (equal to 1.96 S.D.) was 0.21°C, and 0.44°C, respectively. In the field test, involving real competitive racing under severe conditions on a racing circuit, the system functioned well. These results suggest that our novel system has an acceptable performance in a race setting as a reliable tympanic temperature monitor and could help to improve safety of motor sports. © 2013 Springer-Verlag
How do enamelysin and kallikrein 4 process the 32-kDa enamelin?
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74876/1/j.1600-0722.2006.00281.x.pd
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors
Physiological measurements and analyses in motor sports: a preliminary study in racing kart athletes
KAT Ligation for Rapid and Facile Covalent Attachment of Biomolecules to Surfaces
The efficient and bioorthogonal chemical ligation reaction between potassium acyltrifluoroborates (KATs) and hydroxylamines (HAs) was used for the surface functionalization of a self-assembled monolayer (SAM) with biomolecules. An alkane thioether molecule with one terminal KAT group (S-KAT) was synthesized and adsorbed onto a gold surface, placing a KAT group on the top of the monolayer (KAT-SAM). As an initial test case, an aqueous solution of a hydroxylamine (HA) derivative of poly(ethylene glycol) (PEG) (HA-PEG) was added to this KAT-SAM at room temperature to perform the surface KAT ligation. Quartz crystal microbalance with dissipation (QCM-D) monitoring confirmed the rapid attachment of the PEG moiety onto the SAM. By surface characterization methods such as contact angle and ellipsometry, the attachment of PEG layer was confirmed, and covalent amide-bond formation was established by X-ray photoelectron spectroscopy (XPS). In a proof-of-concept study, the applicability of this surface KAT ligation for the attachment of biomolecules to surfaces was tested using a model protein, green fluorescent protein (GFP). A GFP was chemically modified with an HA linker to synthesize HA-GFP and added to the KAT-SAM under aqueous dilute conditions. A rapid attachment of the GFP on the surface was observed in real time by QCM-D. Despite the fact that such biomolecules have a variety of unprotected functional groups within their structures, the surface KAT ligation proceeded rapidly in a chemoselective manner. Our results demonstrate the versatility of the KAT ligation for the covalent attachment of a variety of water-soluble molecules onto SAM surfaces under dilute and biocompatible conditions to form stable, natural amide bonds
Porcine dentin matrix protein 1: gene structure, cDNA sequence, and expression in teeth
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73278/1/j.1600-0722.2006.00284.x.pd
Co-operative mineralization and protein self-assembly in amelogenesis: silica mineralization and assembly of recombinant amelogenins in vitro
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73342/1/j.1600-0722.2006.00288.x.pd
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