Cenozoic tectono-thermal history of the southern Talkeetna Mountains, Alaska: multiple topographic development drivers through time

Thesis (M.S.) University of Alaska Fairbanks, 2018Intraplate mountain ranges can have polyphase topographic development histories reflecting diverse plate boundary conditions. We apply ⁴⁰Ar/³⁹Ar, apatite fission track (AFT) and apatite (U-Th)/He (AHe) geochronology-thermochronology to plutonic and volcanic rocks in the southern Talkeetna Mountains of Alaska to document regional magmatism, rock cooling and inferred exhumation patterns as proxies for the deformation history of this long-lived intraplate mountain range. High-temperature ⁴⁰Ar/³⁹Ar geochronology on muscovite, biotite and K-feldspar from Jurassic granitoids indicates post-emplacement (~158-125 Ma) cooling and Paleocene (~61 Ma) thermal resetting. ⁴⁰Ar/³⁹Ar whole rock volcanic ages and AFT cooling ages in the southern Talkeetna Mountains are predominantly Paleocene-Eocene, suggesting that the Range is partially paleotopography that formed during an earlier tectonic setting. Miocene AHe cooling ages within ~10 km of the Castle Mountain Fault suggest ~2-3 km of vertical displacement that also contributed to mountain building, likely in response to the inboard progression of the subducted Yakutat microplate. Paleocene-Eocene volcanic and exhumation ages across interior southern Alaska north of the Border Ranges Fault System are similar and show no N-S or W-E progressions, suggesting a broadly synchronous and widespread volcanic and exhumation event that conflicts with the proposed diachronous subduction of an active west-east sweeping spreading ridge beneath south-central Alaska. To reconcile this, we propose a new model for the Cenozoic tectonic evolution of southern Alaska. We infer that slab breakoff sub-parallel to the trench and subsequent mantle upwelling drove magmatism, exhumation and rock cooling synchronously across south-central Alaska and played a primary role in the development of the southern Talkeetna Mountains.Undergraduate Research and Scholarly Activity, Geological Society of America, Alaska Geological Society, American Association of Petroleum Geologist

Using First Passage Time Analysis to Identify Foraging Patterns of the Northern Bobwhite

Patterns in movement data can reveal important information relating environmental variables to behavioral mechanisms. First passage time analysis (hereafter; FPT) can be used to quantify the spatial and temporal variation in movements by identifying areas of restricted search behavior based on measuring residence time in an area. It is applicable in studies of foraging ecology and habitat selection because it can empirically quantify behavioral decisions without any a priori assumptions of habitat availability. Furthermore, FPT analysis is simple to implement and interpret; however, the technique has yet to be applied to the northern bobwhite (Colinus virginianus, hereafter bobwhite) because telemetry locations in short (e.g., 30 min) successive time intervals are needed. Our primary objective was to better understand patterns in foraging behavior of bobwhites as it relates to habitat use and improve management. Our secondary objective was to test the efficiency of using FPT analysis on telemetry data collected at different time intervals. Bobwhites were captured during the fall of 2013 and 2014 on a private plantation in South Carolina and fitted with very high frequency (VHF) transmitters (n = 143 and n = 148, respectively). We located coveys at 1 hour (2013) and 30 (2014) minute time intervals during daylight. Bobwhites concentrated their searching efforts to a few hours pre-dusk. Search efforts were proximal to supplemental food sources, with some intra-seasonal variation. Advances in global positioning system (GPS) technology will likely increase opportunities for collecting fine-scale movement data for bobwhites. Understanding techniques such as FPT analysis will enhance our knowledge of northern bobwhite ecology and management

A componential approach to individual differences in hypnotizability

Although responsiveness to hypnotic suggestions (hypnotizability) typically is conceptualized and studied as a singular homogeneous capability, numerous lines of evidence suggest instead that it is a hierarchically structured cognitive capacity comprising a core superordinate ability and ancillary subordinate component abilities. After reviewing current approaches to the measurement of hypnotizability and componential approaches to other cognitive capabilities, we highlight outstanding questions in the field and argue for a componential approach to the study of hypnotizability. Such an approach assumes that hypnotizability is not a unitary construct but is rooted in multiple subabilities that interact to give rise to individual differences that are expressed within specific contexts. We revisit previous componential work on hypnotizability and propose a series of steps by which a componential model can be more rigorously interrogated and integrated with contemporary advances in our understanding of human cognition

Cytomegalovirus Late Protein pUL31 Alters Pre-rRNA Expression and Nuclear Organization during Infection

ABSTRACT The replication cycle of human cytomegalovirus (CMV) leads to drastic reorganization of domains in the host cell nucleus. However, the mechanisms involved and how these domains contribute to infection are not well understood. Our recent studies defining the CMV-induced nuclear proteome identified several viral proteins of unknown functions, including a protein encoded by the UL31 gene. We set out to define the role of UL31 in CMV replication. UL31 is predicted to encode a 74-kDa protein, referred to as pUL31, containing a bipartite nuclear localization signal, an intrinsically disordered region overlapping arginine-rich motifs, and a C-terminal dUTPase-like structure. We observed that pUL31 is expressed with true late kinetics and is localized to nucleolin-containing nuclear domains. However, pUL31 is excluded from the viral nuclear replication center. Nucleolin is a marker of nucleoli, which are membrane-less regions involved in regulating ribosome biosynthesis and cellular stress responses. Other CMV proteins associate with nucleoli, and we demonstrate that pUL31 specifically interacts with the viral protein, pUL76. Coexpression of both proteins altered pUL31 localization and nucleolar organization. During infection, pUL31 colocalizes with nucleolin but not the transcriptional activator, UBF. In the absence of pUL31, CMV fails to reorganize nucleolin and UBF and exhibits a replication defect at a low multiplicity of infection. Finally, we observed that pUL31 is necessary and sufficient to reduce pre-rRNA levels, and this was dependent on the dUTPase-like motif in pUL31. Our studies demonstrate that CMV pUL31 functions in regulating nucleolar biology and contributes to the reorganization of nucleoli during infection. IMPORTANCE Nucleolar biology is important during CMV infection with the nucleolar protein, with nucleolin playing a role in maintaining the architecture of the viral nuclear replication center. However, the extent of CMV-mediated regulation of nucleolar biology is not well established. Proteins within nucleoli regulate ribosome biosynthesis and p53-dependent cellular stress responses that are capable of inducing cell cycle arrest and/or apoptosis, and they are proposed targets for cancer therapies. This study establishes that CMV protein pUL31 is necessary and sufficient to regulate nucleolar biology involving the reorganization of nucleolar proteins. Understanding these processes will help define approaches to stimulate cellular intrinsic stress responses that are capable of inhibiting CMV infection

Refining the Hunting Zone of Hunter-Covey Interface Models

Regulating harvest is important to sustain northern bobwhite (Colinus virginianus) populations. Direct measures to control harvest such as setting fixed proportions (i.e., percent of fall population) are not typically feasible, thus, indirect measures (e.g., managing access, season length) are more commonly used. However, these measures are predicated on relationships between hunter effort and kill rate (K) which is a function of several parameters including: the probability of encountering a covey (p), where p is a function of the effective area hunted (a) divided by that available (A). Thus, a, is a product of the velocity of hunter movement (v), hours spent hunting (h), and the effective width of the hunting zone (w). Velocity and hours spent hunting are easy to quantify, however, estimating w is more difficult and to-date not undertaken. We focused on w, specifically wded, the distance a dog detects a covey assuming the covey is stationary. We assume stationarity such that evasive behaviors can be estimated separately from the olfaction process. The objective of our experiments was to estimate the influence of weather on wded. We used pen-raised bobwhites placed about 150 meters apart to simulate hunts (n = 13) on two study sites. A handler guided a single birddog through the course, downwind from birds, and recorded the distance from the pointed dog to caged birds. Dogs pointed birds (n = 236) at an average distance of 6.2 m (SD = 4.2). Wind speed was positively associated with detection distance (r = 0.19, P \u3c 0.01), while temperature was negatively associated (r = -0.18, P \u3c 0.05). The hunter-covey interface is a dynamic process driven by a myriad of factors. Our results suggest simple weather parameters influence the effective area hunted, therefore, affecting the kill rate that managers want to control

Hunter-Covey Interactions Using Pointing Bird Dogs

Hunting northern bobwhites (Colinus virginianus) with pointing dogs is a long-standing tradition in the Southeastern United States. Despite this rich hunting legacy, a paucity of empirical, behavioral information exists on the interaction between bobwhite coveys, pointing dogs and humans. As such, the efficiency of using pointing dogs to locate bobwhite coveys or an individual covey’s behavioral response to hunting is poorly understood. During 2013 – 2015, we conducted hunts (n = 192) by mode of foot on Tall Timbers Research Station (TTRS, ~1,570 ha) in Leon County, Florida and horseback on a private property (2,023 ha) in Georgetown County, South Carolina. We captured bobwhites (n = 741) and fitted them with activity-switch enabled radio-transmitters, and we tracked coveys prior to, during and after hunts. We used 2 types of global positioning system (GPS) units to collect route data from dogs and hunters (via horseback or foot). We recorded encounter information (e.g., behavior, encounter type such as covey point or wild flush) in the field using a pre-configured application on an iPad and linked spatial data using a geographic information system (i.e., ArcGIS). On average, 52% of all radio-tagged coveys were available (within a dog’s scent radius) during a hunt of which 73% were detected by pointing bird dogs. The overall probability of observing a covey on a hunt was 38% suggesting that most coveys within a hunting course go undetected. Vegetation density did not appear to be an impediment to bobwhite mobility or an important factor in detection of coveys by bird dogs. The potential reduction or manipulation of existing habitats may help to constrain where bobwhite coveys can escape to and covertly improve hunting efficiency. Furthermore, our results imply that a relatively high bobwhite density is required for sportsman to frequently encounter bobwhite coveys during a hunt

Growth of delta-doped layers on silicon CCD/S for enhanced ultraviolet response

The backside surface potential well of a backside-illuminated CCD is confined to within about half a nanometer of the surface by using molecular beam epitaxy (MBE) to grow a delta-doped silicon layer on the back surface. Delta-doping in an MBE process is achieved by temporarily interrupting the evaporated silicon source during MBE growth without interrupting the evaporated p+ dopant source (e.g., boron). This produces an extremely sharp dopant profile in which the dopant is confined to only a few atomic layers, creating an electric field high enough to confine the backside surface potential well to within half a nanometer of the surface. Because the probability of UV-generated electrons being trapped by such a narrow potential well is low, the internal quantum efficiency of the CCD is nearly 100% throughout the UV wavelength range. Furthermore, the quantum efficiency is quite stable