Capillary abnormalities in workers occupationally exposed to ionizing and nonionizing radiations

Cilj studije je bio metodom kapilaroskopije upozoriti na mogući utjecaj ionizacijskog i neionizacijskog zračenja na periferni krvotok profesionalno izloženih osoba. Kapilaroskopski smo pregledali 100 osoba profesionalno izloženih ionizacijskom zračenju, 110 osoba profesionalno izloženih neionizacijskom zračenju i 80 kontrolnih, neizloženih osoba. U obje test-skupine oštećenja mikrocirkulacije su bila statistički značajno učestalija nego u kontrolnoj skupini.The aim of the study was to determine a possible effect of ionizing and nonionizing radiations on peripheral blood flow in occupationally exposed persons by means of capillaroscopic analysis. Altogether 290 subjects were examined. Of these 100 were occupationally exposed to ionizing radiation, 110 were occupationally exposed to nonionizing radiation and 80 control subjects never worked with radiation sources. Statistical analysis showed that microvascular abnormalities occurred significantly more frequently among occupationally exposed persons than in the control group

The frequency of sister chromatid exchanges in V79 chinese hamster cells exposed to heavy metals

Metodom izmjena kromatida sestara (SCE) testirana je mutagena aktivnost Pb2+, Mn2+, Ni2+ i Cd2+. Rezultati su pokazali da u navedenim eksperimentalnim uvjetima olovo i nikal značajnije ne mijenjaju broj SCE, dok mangan, ovisno o dozi, uzrokuje povećan broj izmjena kromatida sestara. Kao izraziti mutagen pokazao se kadmij. Već nakon 2-satne inkubacije stanica V79 kineskog hrčka i s najnižom testiranom koncentracijom kadmija, broj SCE se udvostručuje.The mutagenic activity of Pb2+, Mn2+, Ni2+ and Cd2+ was tested by the method of sister chromatid exchanges (SCE). The results show that in the given experimental conditions lead and nickel did not cause any significant change in the SCE rate, while manganese, depending on the dose, increased the number of exchanges. Cadmium exerted a marked mutagenic action. At the end of a 2-hour incubation of V79 Chinese hamster cells even the lowest tested cadmium concentration brought about the doubling of SCE

The frequency of sister chromatid exchanges in V79 chinese hamster cells exposed to heavy metals

Metodom izmjena kromatida sestara (SCE) testirana je mutagena aktivnost Pb2+, Mn2+, Ni2+ i Cd2+. Rezultati su pokazali da u navedenim eksperimentalnim uvjetima olovo i nikal značajnije ne mijenjaju broj SCE, dok mangan, ovisno o dozi, uzrokuje povećan broj izmjena kromatida sestara. Kao izraziti mutagen pokazao se kadmij. Već nakon 2-satne inkubacije stanica V79 kineskog hrčka i s najnižom testiranom koncentracijom kadmija, broj SCE se udvostručuje.The mutagenic activity of Pb2+, Mn2+, Ni2+ and Cd2+ was tested by the method of sister chromatid exchanges (SCE). The results show that in the given experimental conditions lead and nickel did not cause any significant change in the SCE rate, while manganese, depending on the dose, increased the number of exchanges. Cadmium exerted a marked mutagenic action. At the end of a 2-hour incubation of V79 Chinese hamster cells even the lowest tested cadmium concentration brought about the doubling of SCE

Significance of ultrasound and ultrasound-guided fine-needle aspiration for the detection of laryngeal occult metastases

Background/Aim. To evaluate ultrasound criteria based on a node size, shape, vascularity and cytology findings with respect to their value for the comparative determination of metastatic lymph nodes in laryngeal carcinoma. Methods. A prospective study included 30 patients with laryngeal squamous cell carcinoma without node enlargement on computerized tomography, at the Department of Otorhinolaryngology, the University Hospital, Zemun. Thirty-six neck lymph nodes were evaluated sonographically and aspirated with an ultrasound-guided fine-needle. They were examined cytologically and/or histopathologically and compared to the sonographic assessment of their malignancy. Results. Of the 36 neck lymph nodes evaluated cytologically, the 13 were found to be with a metastatic deposit. The assessment of a lymph node malignancy using the parameter of size had the sensitivity of 84%, the specificity of 70%, and the reliability of 75%. Using the criteria of a lymph node shape for the assessment of malignancy, the sensitivity of 61%, specificity of 65%, and the reliability of 64% were achieved. The evaluation of a lymph node vascularity by the use of the effect of Doppler showed the sensitivity of 69%, the specificity of 95%, and the reliability of 86%. Concluson. Ultrasound and ultrasound-guided fine-needle aspiration cytology should be always used for the preoperative staging and for the postoperative follow-up of the status of the neck with cancer of the larynx becouse of their high accuracy, availability and semiinvasivity, and in order to enhance the reliability of the evaluation of the malignant disease progression