Identification of seminal plasma proteins in Gir bulls

Submitted by Ana Helena Freire ([email protected]) on 2016-06-17T14:13:38Z No. of bitstreams: 1 Folhadella e Ramos Rev Bras Reprod Anim BH 2013.pdf: 6443091 bytes, checksum: d933daa6f89658924fbaeffdeec2049f (MD5)Approved for entry into archive by Ivan Neves Junior ([email protected]) on 2016-08-09T14:16:20Z (GMT) No. of bitstreams: 1 Folhadella e Ramos Rev Bras Reprod Anim BH 2013.pdf: 6443091 bytes, checksum: d933daa6f89658924fbaeffdeec2049f (MD5)Made available in DSpace on 2016-08-09T14:16:20Z (GMT). No. of bitstreams: 1 Folhadella e Ramos Rev Bras Reprod Anim BH 2013.pdf: 6443091 bytes, checksum: d933daa6f89658924fbaeffdeec2049f (MD5) Previous issue date: 2013Submitted by Gilvan Almeida ([email protected]) on 2016-08-17T14:11:11Z No. of bitstreams: 3 Folhadella e Ramos Rev Bras Reprod Anim BH 2013.pdf.txt: 8 bytes, checksum: 8d1b69dd9bdc9df4a8073c7a8193c7af (MD5) Folhadella e Ramos Rev Bras Reprod Anim BH 2013.pdf: 6443091 bytes, checksum: d933daa6f89658924fbaeffdeec2049f (MD5) license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)Approved for entry into archive by Fátima Fandinho Montes ([email protected]) on 2018-09-27T18:53:09Z (GMT) No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) Folhadella e Ramos Rev Bras Reprod Anim BH 2013.pdf: 6443091 bytes, checksum: d933daa6f89658924fbaeffdeec2049f (MD5) Folhadella e Ramos Rev Bras Reprod Anim BH 2013.pdf.txt: 8 bytes, checksum: 8d1b69dd9bdc9df4a8073c7a8193c7af (MD5)Made available in DSpace on 2018-09-27T18:53:09Z (GMT). No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) Folhadella e Ramos Rev Bras Reprod Anim BH 2013.pdf: 6443091 bytes, checksum: d933daa6f89658924fbaeffdeec2049f (MD5) Folhadella e Ramos Rev Bras Reprod Anim BH 2013.pdf.txt: 8 bytes, checksum: 8d1b69dd9bdc9df4a8073c7a8193c7af (MD5) Previous issue date: 2013Fundação Oswaldo Cruz. Centro de Criação de Animais de Laboratório. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas.Belo Horizonte, MG, Brasil.Centro de Educação Superior a Distância do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Embrapa. Juiz de Fora, MG, Brasil.Embrapa. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Centro de Criação de Animais de Laboratório. Rio de Janeiro, RJ, Brasil.Universidade Federal de Juiz de Fora. Juiz de Fora, MG, Brasil.Embrapa. Juiz de Fora, MG, Brasil.Embrapa. Juiz de Fora, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas.Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas.Belo Horizonte, MG, Brasil.Central Lagoa da Serra. Sertãozinho, SP, Brasil.Central Lagoa da Serra. Sertãozinho, SP, Brasil.Estudou-se o perfil de proteínas totais (PLB) e proteínas ligadoras de heparina (PLH) do plasma seminal de touros da raça Gir e suas associações com parâmetros andrológicos e fertilidade in vitro. A concentração da PLB variou de 4,1 a 167,9 mg/mL e da PLH entre 0,02096 e 0,19025 (%). O perfil cromatográfico apresentou nove picos de afinidade para PLB e cinco picos para PLH. As associações significativas foram: PLB x taxa de blastocistos, PLH do pico 1 x taxa de blastocistos, PLH do pico 5 x taxa de clivagem e blastocistos. Nenhuma proteína estudada apresentou associação com parâmetros andrológicos.It was studied the seminal plasma total protein (TP) and heparin binding protein (PLH) profiles of Gyr bulls and their associations with andrological parameters and in vitro fertility. Total protein concentration varied between 4,1 a 167,9 mg/ml and heparin binding protein 0,02096 to 0,19025 (%). Chromatographic profile of TP presented nine peaks. PLH presented five affinity peaks. Associations were for: total proteins x blastocysts rate, peak 1 heparin binding proteins x blastocysts rate, peak 5 heparin binding protein x cleavage rate and blastocysts rate. However no protein showed association with andrological parameters

Effect of maternal heat-stress on follicular growth and oocyte competence in Bos indicus cattle

The objective was to determine whether exposure of Gir (Bos indicus) cows to heat-stress (HS) causes immediate and delayed deleterious effect on follicular dynamics, hormonal profile and oocyte competence. The cows were kept in tie-stalls for an adaptive thermoneutral period of 28 days (Phase I, Days -28 to -1). In Phase II (Days 0-28) cows were randomly allocated into control (CG, n = 5) and HS (HS, n = 5) treatments. The HS cows were placed in an environmental chamber at '38 GRAUS'C and 80% relative humidity (RH) during the day and '30 GRAUS'C, 80% RH during the night for 28 days. The CG group was maintained in shaded tie-stalls (ambient temperature) for 28 days. During Phase III (Days 28-147) animals were placed in tie-stalls (Days 28-42) followed by pasture (Days 42-147) under thermoneutrality. In each phase, weekly ovum pick up (OPU) sessions were to evaluate follicular development, morphology of cumulus-oocyte complexes (COCs), and developmental competence after in vitro maturation, fertilization, and culture. Serum concentrations of progesterone ('P IND. 4') and cortisol were evaluated by radioimmunoassay. Exposure of Gir cows to HS had no immediate effect on reproductive function, but exerted a delayed deleterious effect on ovarian follicular growth, hormone concentrations, and oocyte competence. Heat-stress increased the diameter of the first and second largest follicles from Days 28 to 49. Indeed, HS increased the number of >9 mm follicles (characterized as follicular codominance) during this phase. Cows exposed to HS had longer periods of non-cyclic activity ('P IND. 4' < 1 ng/mL), as well as shorter estrous cycles. However, HS did not affect cortisol concentration as compared to CG. Although HS had no significant effect on cleavage rate, it reduced blastocyst development during Phase III. In conclusion, long-term exposure of B. indicus cattle to HS had a delayed deleterious effect on ovarian follicular dynamics and oocyte competenc