19 research outputs found
Applicability ofMathematicalModels in Defining the Behaviour Kinetics Distinction Among Microbial Strains
Mathematical models were applied to define the behaviour kinetics distinction among microbial strains. In the first series of experiments the growth kinetics of microbial colonies of several S. rimosus mutant strains cultivated on agar plates were compared. Then, the interest was focused on the chosen two strains, in order to express mathematically their differences with respect to their colony growth and antibiotic biosynthesis kinetics. Finally, the behaviour of selected three S. rimosus derivative strains at different culture conditions was subjected to the study, with an aim to define strain distinction parameters. Mathematical models based on the three-dimensional
growth concept and describing the microorganism growth, substrate uptake and antibiotic biosynthesis kinetics were developed. The computer simulation was applied to verify the applicability of mathematical models. The excellent agreement of computer simulation with experimental data confirmed the hypothesis that the kinetics parameters can be successfully applied to define the behaviour distinction among different S. rimosus strains. In the case of selected three strains, S. rimosus R6–500, S. rimosus MV9R-1 and
MV9R-2, it was established that they can be distinguished by their growth kinetics parameters, their substrate uptake kinetics parameters and their antibiotic biosynthesis kineticsparameters. The strain S. rimosus R6–500 showed to be superior with respect to all
kinetics parameters, the strain S. rimosus MV9R-2 showed to be slightly inferior to it, whereas the strain S. rimosus MV9R-1 showed to be inferior with respect to the both mentioned strains, especially because it showed the pronounced active biomass reduction
rate at all investigated culture conditions. Based on these and the corresponding previous results one can conclude that appropriate mathematical models can be recommended for defining parameters of microbial behaviour distinction among different microbial strains
of S. rimosus species
Novel hybrid polyketide compounds produced by genetic engineering of the oxytetracycline biosynthetic pathway. Food Technol. Biotechnol
Summary The whole oxytetracycline (OTC
THE STREPTOMYCES-GHANAENSIS LOW COPY PLASMID PSG2 AND ITS USE FOR VECTOR CONSTRUCTION
WOHLLEBEN W, Pühler A. THE STREPTOMYCES-GHANAENSIS LOW COPY PLASMID PSG2 AND ITS USE FOR VECTOR CONSTRUCTION. ARCHIVES OF MICROBIOLOGY. 1987;148(4):298-304