Enumeration of bone marrow and blood Lin−/Sca1+ cell numbers following stroke and SDF1-A Antibody.

<p>(A) Twenty-four hour post stroke+SDF1-A antibody mice show a marked increase in the number of Lin−/Sca1+ cells present in the bone marrow compared to controls. (B) Twenty-four hour post stroke mice showed a marked decrease in the mobilization of these cells to the blood. * P<0.05.</p

Administration of Lin−/Sca1+ cells post stroke.

<p>A. Administration of Lin−/Sca1+ cells post stroke significantly reduced infarct volume at 24 hours post stroke (*P<0.05). B. Administration of the Lin−/Sca1+ cells with an SDF1-A antibody (AB) no longer demonstrates a beneficial reduction in the infarct volume (p = NS).</p

SDF1-A levels in serum and brain.

<p>(A) Serum SDF1-A levels reached significance at 24 hours post surgery. (B) Brain tissue levels of SDF1-A demonstrated significant elevations at both 4 and 24 hours post stroke compared to sham animals. *P<0.05.</p

Enumeration of bone marrow and blood Lin−/Sca1+ cell numbers following stroke.

<p>(A) Twenty-four hour post stroke mice show a marked increase in the number of Lin−/Sca1+ cells present in the bone marrow compared to controls. (B) Twenty-four hour post stroke mice also showed a marked increase in the mobilization of these cells to the blood. * P<0.05.</p

Y chromosome Positive cells in brain.

<p>Male Lin−/Sca1+ cells injected IV post stroke were counted in female ischemic hemisphere at 24 hours post stroke; Animals which received SDF1-A antibody in addition to the IV Lin−/Sca1+ cells, had a significantly lower number of cells detected in the brain by FISH (*P<0.05). Green = Y chromosome.</p