5,765 research outputs found
Biodegradable ion-exchange microspheres based on modified polylysines
Poly-L-lysine was synthesized via a triethylamine initiated ring-opening polymerization of Z-L-lysine-N'~-carboxyanhydride,\ud
followed by deprotection of the E-amino group. Subsequently the polylysine was sulfamated using a pyridinium-sulfate complex to obtain polymers with varying degrees of sulfamation ranging from 0 to 100%. Cytotoxicity of these materials was tested using tetrazolium metabolism (MTI') assays with B16F10 and P388 cell lines. Cytotoxicity of sulfamated polylysines with a degree of sulfamation of 80% and higher was significantly reduced as compared with the native polylysines. In both cell lines, LDso of the sulfamated materials was higher than 5 mg/ml, which was the highest dose tested. LDso of the native polylysines was lower than 0.1 mg/ml in the case of B16F10 and lower than 0.01 mg/ml in the case of P388 cells. Sulfamated polylysines with a degree of sulfamation of 80% were used to prepare microspheres (SPLMS). The microspheres were stabilized using glutaraldehyde or oxidized dextran as a crosslinking agent. The swelling ratio (defined as V~wollen/Vdr~ed) of the SPLMS in aqueous media decreased with increasing ionic strength and crosslink density. The pH (ranging from 3 to 11) had no influence on the swelling ratio of SPLMS. The maximal swelling ratio was approximately 35 (SPLMS crosslinked with 0.5% glutaraldehyde in distilled water). SPLMS could be loaded with adriamycin up to a payload of 60%, which was not influenced by the crosslinking method. The adriamycin release was controlled by the ionic strength of the release medium: no drug was released in non-ionic medium such as distilled water, while 80% of the drug was released in phosphate buffered saline. This effect of the change in ionic strength could be applied to prepare a microsphere suspension in non-ionic medium such as 5% glucose solution, which does not contain free adriamycin. The drug would only be release after intra-arterial administration of this suspension, due to\ud
the presence of the blood
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Rapidity Gaps Between Jets
An excess of events with a rapidity gap between jets, over what would be
expected from non-diffractive processes, has been observed at HERA. A process
based on a perturbative QCD calculation of colour singlet exchange has been
added to HERWIG. With this addition, HERWIG is able to describe the number of
events with a gap between jets over the number without a gap. This gap fraction
is predicted to rise at large rapidity intervals between jets which would only
be visible if the detector coverage were increased.Comment: 5 pages including 3 figures. To appear in the conference proceedings
of the Workshop "Future Physics at HERA
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Southern California Beach Processes Study - Torrey Pines Beach Nourishment Study 7th Quarterly Report
Computational challenges of systems biology
Progress in the study of biological systems such as the heart, brain, and liver will require computer scientists to work closely with life scientists and mathematicians. Computer science will play a key role in shaping the new discipline of systems biology and addressing the significant computational challenges it poses
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