Microbial Lipid Production: Screening With Yeasts Grown On Brazilian Molasses.

Rhodotorula glutinis CCT 2182, Rhodosporidium toruloides CCT 0783, Rhodotorula minuta CCT 1751 and Lipomyces starkeyi DSM 70296 were evaluated for the conversion of sugars from Brazilian molasses into single-cell oil (SCO) feedstock for biodiesel. Pulsed fed-batch fermentations were performed in 1.65 l working volume bioreactors. The maximum specific growth rate (µmax), lipid productivity (Pr) and cellular lipid content were, respectively, 0.23 h(-1), 0.41 g l(-1) h(-1), and 41% for Rsp. toruloides; 0.20 h(-1), 0.27 g l(-1) h(-1), and 36% for Rta. glutinis; 0.115 h(-1), 0.135 g l(-1) h(-1), and 27 % for Rta. minuta; and 0.11 h(-1), 0.13 g l(-1) h(-1), and 32% for L. starkeyi. Based on their microbial lipid productivity, content, and profile, Rsp. toruloides and Rta. glutinis are promising candidates for biodiesel production from Brazilian molasses. All the oils from the yeasts were similar to the composition of plant oils (rapeseed and soybean) and could be used as raw material for biofuels, as well as in food and nutraceutical products.362433-4

Microbial lipid production: screening with yeasts grown on Brazilian molasses

Rhodotorula glutinis CCT 2182, Rhodosporidium toruloides CCT 0783, Rhodotorula minuta CCT 1751 and Lipomyces starkeyi DSM 70296 were evaluated for the conversion of sugars from Brazilian molasses into single-cell oil (SCO) feedstock for biodiesel. Pulsed fed-batch fermentations were performed in 1.65 l working volume bioreactors. The maximum specific growth rate (A mu(max)), lipid productivity (Pr) and cellular lipid content were, respectively, 0.23 h(-1), 0.41 g l(-1) h(-1), and 41 % for Rsp. toruloides; 0.20 h(-1), 0.27 g l(-1) h(-1), and 36 % for Rta. glutinis; 0.115 h(-1), 0.135 g l(-1) h(-1), and 27 % for Rta. minuta; and 0.11 h(-1), 0.13 g l(-1) h(-1), and 32 % for L. starkeyi. Based on their microbial lipid productivity, content, and profile, Rsp. toruloides and Rta. glutinis are promising candidates for biodiesel production from Brazilian molasses. All the oils from the yeasts were similar to the composition of plant oils (rapeseed and soybean) and could be used as raw material for biofuels, as well as in food and nutraceutical products361224332442sem informaçã

Single cell oil production integrated to a sugarcane-mill: Conceptual design, process specifications and economic analysis using molasses as raw material

This study aimed to assess the single-cell oil production integrated to a sugarcane-mill and molasses as main raw material. Rhodotorula glutinis lipid production with low-cost raw sugarcane molasses was accessed. The envisage process relies in an optimized sugarcane molasses culture media in a fed-batch strategy to achieve a high-cell density high-lipid cell content. A Process Flow Design (PFD) integrated to a typical Brazilian sugar mill was proposed according to fermentation experiments. Performed economic analysis provided data for discussion on strategic equipment that can facilitate the viability to the process. Typical results of established protocol were total biomass concentration 62.25 g l−1, lipid productivity 0.42 g l−1 h−1, sugar conversion yield to lipids 0.21 g of lipid g−1 of total reducing sugar. Microbial lipid production and defatted biomass plant production was proposed at a nominal capacity of respectively 16,720 ton and 21,600 ton per year, running 8300 h per year. The projected selling prices of these products were US$1,300.00/ton of lipid and US$ 500.00/ton of defatted yeast. It was demonstrated that the industrial plant was potentially attractive when capital investment cost was decreased with the use of low cost epoxy-lined carbon steel stirred bioreactor. In this alternative, the internal rate of return (IRR) will be 24.61% leading the NPV (at 7% interest rate) of US$ 67,797,000/year (before taxes)89478485FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informaçã

A differential evolution approach to estimate parameters in a temperature-dependent kinetic model for second generation ethanol production under high cell density with Spathaspora passalidarum

An unstructured–unsegregated temperature-dependent kinetic model was developed and validated to describe ethanol fermentation in a mixture of xylose and glucose for the nonconventional yeast Spathaspora passalidarum NRRL Y-27907 under high cell density in the temperature range of 26–32 °C. The kinetic model consisted of 13 equations and 16 kinetic parameters, describing cell growth, individual xylose and glucose uptake and product formation. Global parametric estimation was executed through a differential evolution algorithm. Temperature-dependent parameters were identified and adjusted to Arrhenius-type equations as a function of temperature. The overall  r2 of the process calibration and validation were 0.972 and 0.959, respectively, indicating that the model satisfactorily described the process in the temperature range investigated. Furthermore, using a 3D projection of the model to simulate ethanol production, it was concluded that temperatures between 30 and 32 °C resulted in the highest productivities161COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informação2015/20630-4; 2016/14567-0; 2017/04997-