Synchronous Phase Shift at LHC

The electron cloud in vacuum pipes of accelerators of positively charged particle beams causes a beam energy loss which could be estimated from the synchronous phase. Measurements done with beams of 75 ns, 50 ns, and 25 ns bunch spacing in the LHC for some fills in 2010 and 2011 show that the average energy loss depends on the total beam intensity in the ring. Later measurements during the scrubbing run with 50 ns beams show the reduction of the electron cloud due to scrubbing. Finally, measurements of the individual bunch phase give us information about the electron cloud build-up inside the batch and from batch to batch.Comment: Presented at ECLOUD'12: Joint INFN-CERN-EuCARD-AccNet Workshop on Electron-Cloud Effects, La Biodola, Isola d'Elba, Italy, 5-9 June 201

SASICE: Safety and sustainability in civil engineering

The performance of the built environment and the construction sector are of major importance in Europe’s long term goals of sustainable development in a changing climate. At the same time, the quality of life of all European citizens needs to be improved and the safety of the built environment with respect to man-made and natural hazards, such as flooding and earthquakes, needs to be ensured. Education has a central role to play in the transformation of a construction sector required to meet increasing demands with regard to safety and sustainability. In this work, the SASICE project is presented. The aim of this project is to promote the integration of safety and sustainability in civil engineering education. The project is organised in the context of the Lifelong Learning Programme, funded by the European Community. The coordinator organisation is the University of Bologna. Nine partner universities from different countries are involved in this transnational project. The universities participating to the project constitute a network of high level competences in the civil engineering area, with several opportunities to improve lifelong learning adopting different media: joint curricula, teaching modules and professor and student exchanges. As a response to the challenge regarding new educational methods in sustainable engineering, teaching modules are developed in 4 thematic areas: (1) Safety in construction, (2) Risk induced by Natural Hazards Assessment, (3) Sustainability in construction, and (4) Sustainability at the territorial level. The development of the teaching modules is based on an extensive analysis of the need for highly qualified education on Safety and Sustainability involving all relevant stakeholders (European and national authorities, companies, research institutes, professional organizations, and universities).The main target is enabling students to introduce these advanced topics in their study plans and curricula and reach, at the end of their studies, a specific skill and expertise in safety and sustainability in Civil Engineering. With our natural resources fading away and our infrastructure in dire need of repair, new trends and challenges in civil engineering education in the concept of “Sustainable Development” are needed to be adressed.<br/

Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections

Author summary Neglected tropical diseases (NTDs) affecting humans and/or domestic animals severely impair the socio-economic development of endemic areas. One of these diseases, animal trypanosomosis, affects livestock and is caused by the parasites of the Trypanosoma genus. The most widespread causative agent of animal trypanosomosis is T. evansi, which is found in large parts of the world (Africa, Asia, South America, Middle East, and the Mediterranean). Proper control and treatment of the disease requires the availability of reliable and sensitive diagnostic tools. DNA-based detection techniques are powerful and versatile in the sense that they can be tailored to achieve a high specificity and usually allow the reliable detection of low amounts of parasite genetic material. However, many DNA-based methodologies (such as PCR) require trained staff and well-equipped laboratories, which is why the research community has actively investigated in developing amplification strategies that are simple, fast, cost-effective and are suitable for use in minimally equipped laboratories and field settings. In this paper, we describe the development of a diagnostic test under a dipstick format for the specific detection of T. evansi, based on a DNA amplification principle (Recombinase Polymerase Amplification aka RPA) that meets the above-mentioned criteria. Background Animal trypanosomosis caused by Trypanosoma evansi is known as "surra" and is a widespread neglected tropical disease affecting wild and domestic animals mainly in South America, the Middle East, North Africa and Asia. An essential necessity for T. evansi infection control is the availability of reliable and sensitive diagnostic tools. While DNA-based PCR detection techniques meet these criteria, most of them require well-trained and experienced users as well as a laboratory environment allowing correct protocol execution. As an alternative, we developed a recombinase polymerase amplification (RPA) test for Type A T. evansi. The technology uses an isothermal nucleic acid amplification approach that is simple, fast, cost-effective and is suitable for use in minimally equipped laboratories and even field settings. Methodology/Principle findings An RPA assay targeting the T. evansi RoTat1.2 VSG gene was designed for the DNA-based detection of T. evansi. Comparing post-amplification visualization by agarose gel electrophoresis and a lateral flow (LF) format reveals that the latter displays a higher sensitivity. The RPA-LF assay is specific for RoTat1.2-expressing strains of T. evansi as it does not detect the genomic DNA of other trypanosomatids. Finally, experimental mouse infection trials demonstrate that the T. evansi specific RPA-LF can be employed as a test-of-cure tool