A Universal Live Cell Barcoding-Platform for Multiplexed Human Single Cell Analysis.

Single-cell barcoding enables the combined processing and acquisition of multiple individual samples as one. This maximizes assay efficiency and eliminates technical variability in both sample preparation and analysis. Remaining challenges are the barcoding of live, unprocessed cells to increase downstream assay performance combined with the flexibility of the approach towards a broad range of cell types. To that end, we developed a novel antibody-based platform that allows the robust barcoding of live human cells for mass cytometry (CyTOF). By targeting both the MHC class I complex (beta-2-microglobulin) and a broadly expressed sodium-potassium ATPase-subunit (CD298) with platinum-conjugated antibodies, human immune cells, stem cells as well as tumor cells could be multiplexed in the same single-cell assay. In addition, we present a novel palladium-based covalent viability reagent compatible with this barcoding strategy. Altogether, this platform enables mass cytometry-based, live-cell barcoding across a multitude of human sample types and provides a scheme for multiplexed barcoding of human single-cell assays in general

Strong photon non-linearities and photonic Mott insulators

We show, that photon non-linearities in electromagnetically induced transparency can be at least one order of magnitude larger than predicted in all previous approaches. As an application we demonstrate that, in this regime they give rise to very strong photon - photon interactions which are strong enough to make an experimental realization of a photonic Mott insulator state feasible in arrays of coupled ultra high-Q micro-cavities.Comment: minor changes, to appear in Phys. Rev. Let

Direct sampling of complex landscapes at low temperatures: the three-dimensional +/-J Ising spin glass

A method is presented, which allows to sample directly low-temperature configurations of glassy systems, like spin glasses. The basic idea is to generate ground states and low lying excited configurations using a heuristic algorithm. Then, with the help of microcanonical Monte Carlo simulations, more configurations are found, clusters of configurations are determined and entropies evaluated. Finally equilibrium configuration are randomly sampled with proper Gibbs-Boltzmann weights. The method is applied to three-dimensional Ising spin glasses with +- J interactions and temperatures T<=0.5. The low-temperature behavior of this model is characterized by evaluating different overlap quantities, exhibiting a complex low-energy landscape for T>0, while the T=0 behavior appears to be less complex.Comment: 9 pages, 7 figures, revtex (one sentence changed compared to v2

No spin-glass transition in the "mobile-bond" model

The recently introduced ``mobile-bond'' model for two-dimensional spin glasses is studied. The model is characterized by an annealing temperature T_q. On the basis of Monte Carlo simulations of small systems it has been claimed that this model exhibits a non-trivial spin-glass transition at finite temperature for small values of T_q. Here the model is studied by means of exact ground-state calculations of large systems up to N=256^2. The scaling of domain-wall energies is investigated as a function of the system size. For small values T_q<0.95 the system behaves like a (gauge-transformed) ferromagnet having a small fraction of frustrated plaquettes. For T_q>=0.95 the system behaves like the standard two-dimensional +-J spin-glass, i.e. it does NOT exhibit a phase transition at T>0.Comment: 4 pages, 5 figures, RevTe

Ground-state clusters of two-, three- and four-dimensional +-J Ising spin glasses

A huge number of independent true ground-state configurations is calculated for two-, three- and four-dimensional +- J spin-glass models. Using the genetic cluster-exact approximation method, system sizes up to N=20^2,8^3,6^4 spins are treated. A ``ballistic-search'' algorithm is applied which allows even for large system sizes to identify clusters of ground states which are connected by chains of zero-energy flips of spins. The number of clusters n_C diverges with N going to infinity. For all dimensions considered here, an exponential increase of n_C appears to be more likely than a growth with a power of N. The number of different ground states is found to grow clearly exponentially with N. A zero-temperature entropy per spin of s_0=0.078(5)k_B (2d), s_0=0.051(3)k_B (3d) respectively s_0=0.027(5)k_B (4d) is obtained.Comment: large extensions, now 12 pages, 9 figures, 27 reference

Systematic Mapping of the Hubbard Model to the Generalized t-J Model

The generalized t-J model conserving the number of double occupancies is constructed from the Hubbard model at and in the vicinity of half-filling at strong coupling. The construction is realized by a self-similar continuous unitary transformation. The flow equation is closed by a truncation scheme based on the spatial range of processes. We analyze the conditions under which the t-J model can be set up and we find that it can only be defined for sufficiently large interaction. There, the parameters of the effective model are determined.Comment: 16 pages, 13 figures included. v2: Order of sections changed. Calculation and discussion of apparent gap in Section IV.A correcte

Few-Particle Effects in Semiconductor Quantum Dots: Observation of Multi-Charged-Excitons

We investigate experimentally and theoretically few-particle effects in the optical spectra of single quantum dots (QDs). Photo-depletion of the QD together with the slow hopping transport of impurity-bound electrons back to the QD are employed to efficiently control the number of electrons present in the QD. By investigating structurally identical QDs, we show that the spectral evolutions observed can be attributed to intrinsic, multi-particle-related effects, as opposed to extrinsic QD-impurity environment-related interactions. From our theoretical calculations we identify the distinct transitions related to excitons and excitons charged with up to five additional electrons, as well as neutral and charged biexcitons.Comment: 4 pages, 4 figures, revtex. Accepted for publication in Physical Review Letter

Ground-State Properties of a Heisenberg Spin Glass Model with a Hybrid Genetic Algorithm

We developed a genetic algorithm (GA) in the Heisenberg model that combines a triadic crossover and a parameter-free genetic algorithm. Using the algorithm, we examined the ground-state stiffness of the $\pm J$ Heisenberg model in three dimensions up to a moderate size range. Results showed the stiffness constant of $\theta = 0$ in the periodic-antiperiodic boundary condition method and that of $\theta \sim 0.62$ in the open-boundary-twist method. We considered the origin of the difference in $\theta$ between the two methods and suggested that both results show the same thing: the ground state of the open system is stable against a weak perturbation.Comment: 11 pages, 5 figure

A new method for analyzing ground-state landscapes: ballistic search

A ``ballistic-search'' algorithm is presented which allows the identification of clusters (or funnels) of ground states in Ising spin glasses even for moderate system sizes. The clusters are defined to be sets of states, which are connected in state-space by chains of zero-energy flips of spins. The technique can also be used to estimate the sizes of such clusters. The performance of the method is tested with respect to different system sizes and choices of parameters. As an application the ground-state funnel structure of two-dimensional +or- J spin glasses of systems up to size L=20 is analyzed by calculating a huge number of ground states per realization. A T=0 entropy per spin of s_0=0.086(4)k_B is obtained.Comment: 10 pages, 11 figures, 35 references, revte