Poultry Nutrition and Feeding Poultry Production in Ohio

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Department of Animal Sciences research and reviews: poultry and swine

Skeletal muscle collagen organization may be associated with proteoglycan expression / Sandra G. Velleman -- Influence of adult body weight and egg weight on hatching time in selected and random bred control lines of turkeys / K. E. Nestor and D. O. Noble -- Effect of crossing a line selected for increased shank width with two commercial sire lines on performance and walking ability of turkeys / Karl E. Nestor and John W. Anderson -- The energy used for maintenance each day by broiler chickens / David Latshaw and Matthew Toussant -- Variation at microsatellite loci in the large white, Yorkshire, and Hampshire breeds of swine / S. L. Kacirek, K. M. Irvin, P. I. Dimsoski, M. E. Davis, and H. C. Hines -- The estimation of maternal and individual heterosis in Yorkshire, large white, and Hampshire swine and their crosses / M. J. Barhorst, K. M. Irvin, S. J. Moeller, and S. M. Neal -- Investigation of the estrogen receptor gene and its association with reproductive tract traits in swine / B. Isler, K. M. Irvin, and S. M. Neal -- Effects of exogenous testosterone on follicular responsiveness to gonadotrophins during the luteal phase of the estrous cycle in gilts / M. B. Cunningham and W. F. Pope -- Evaluation of the further-processing quality of ham muscles from Hampshire hogs / C. L. Knipe, D. L. Meeker, B. D. Paxton, S. J. Moeller, K. M. Irvin, D. M. Wulf, and R. C. Emnett -- Frequency and effects of the napole gene in the U.S. pork industry / D. L. Meeker, S. J. Moeller, K. M. Irvin, D. M. Wulf, C. L. Knipe, and R. C. Emnet

Sequence-indexed mutations in maize using the UniformMu transposon-tagging population

<p>Abstract</p> <p>Background</p> <p>Gene knockouts are a critical resource for functional genomics. In Arabidopsis, comprehensive knockout collections were generated by amplifying and sequencing genomic DNA flanking insertion mutants. These Flanking Sequence Tags (FSTs) map each mutant to a specific locus within the genome. In maize, FSTs have been generated using DNA transposons. Transposable elements can generate unstable insertions that are difficult to analyze for simple knockout phenotypes. Transposons can also generate somatic insertions that fail to segregate in subsequent generations.</p> <p>Results</p> <p>Transposon insertion sites from 106 UniformMu FSTs were tested for inheritance by locus-specific PCR. We confirmed 89% of the FSTs to be germinal transposon insertions. We found no evidence for somatic insertions within the 11% of insertion sites that were not confirmed. Instead, this subset of insertion sites had errors in locus-specific primer design due to incomplete or low-quality genomic sequences. The locus-specific PCR assays identified a knockout of a 6-phosphogluconate dehydrogenase gene that co-segregates with a seed mutant phenotype. The mutant phenotype linked to this knockout generates novel hypotheses about the role for the plastid-localized oxidative pentose phosphate pathway during grain-fill.</p> <p>Conclusion</p> <p>We show that FSTs from the UniformMu population identify stable, germinal insertion sites in maize. Moreover, we show that these sequence-indexed mutations can be readily used for reverse genetic analysis. We conclude from these data that the current collection of 1,882 non-redundant insertion sites from UniformMu provide a genome-wide resource for reverse genetics.</p