Role of eisosomes in the necrotrophic fungus Alternaria brassicicola

lternaria brassicicola is a fungal necrotroph responsible for the Brassicaceae dark spot disease. This fungus is a seed-borne pathogen that only affects the aerial parts of host plants causing great damages with major incidence on yield and product quality. Its transmission to seed is a major component of pathogen fitness promoting the dispersal and long-term survival of the fungus. Recently, we showed that several eisosomal protein encoding genes were overexpressed when germinated spores of A.brassicicola were exposed to osmotic and hydric stresses, which are the main constraints encountered by the fungus during the seed colonization process. MCC/eisosomes are membrane microdomains whose function is still unclear. They have been proposed to participate in the plasma membrane function by regulating the homeostasis of lipids and would promote the recruitment of specific proteins and their subsequent protection from endocytosis. Here, we deciphered the potential involvement of eisosome in pathogenicity using a reverse genetic approach by generating and characterizing mutants deficient for key eisosomal proteinencoding genes (?pil1, ?nce102, ?lsp1 and ?pil1?lsp1). In parallel, these proteins have been fused to GFP to monitor their cellular location during the plant infection and following the exposure to stresses. Depending on the mutants, the leaf and silique colonization processes were impaired by comparison to the wild-type. We also showed a strong impact of MCC/eisosome proteinmutations on the generation of appressoria-like structures

Genome sequence of the necrotrophic plant pathogen Alternaria brassicicola Abra43

Alternaria brassicicola causes dark spot (or black spot) disease, which is one of the most common and destructive fungal diseases of Brassicaceae spp. worldwide. Here, we report the draft genome sequence of strain Abra43. The assembly comprises 29 scaffolds, with an N50 value of 2.1 Mb. The assembled genome was 31,036,461 bp in length, with a G+C content of 50.85%